From c69b727ed9d41ea0c72f0d3573f816e45e0ae803 Mon Sep 17 00:00:00 2001 From: Soothspider Date: Wed, 27 Mar 2024 14:34:34 -0700 Subject: [PATCH] AI Captions - March 27 - Governed by Stories VENTER 2 --- ... by Stories VENTER II -- (26 Mar 2024).vtt | 4055 +++++++++++++++++ .../README.md | 5 + 2 files changed, 4060 insertions(+) create mode 100755 twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024).vtt create mode 100644 twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/README.md diff --git a/twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024).vtt b/twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024).vtt new file mode 100755 index 0000000..2c0a658 --- /dev/null +++ b/twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024).vtt @@ -0,0 +1,4055 @@ +WEBVTT + +00:00.000 --> 00:13.000 +I may need to just briefly check to see if I can find myself on YouTube and then see + +00:13.000 --> 00:24.120 +what's going on here should be live right now but I'm not which is weird go live I + +00:24.200 --> 00:30.080 +should be live right now because I using that same key excellent connection it says + +00:30.080 --> 00:39.280 +so why am I not live it says viewers will be able to find your stream once you go + +00:39.280 --> 00:53.840 +live but the privacy is unlisted so it should be public done save but I'm not + +00:53.840 --> 01:04.520 +there I don't see any copy I'm gonna stop this and I'm gonna modify it I'm + +01:04.520 --> 01:09.100 +gonna put this key in I'm gonna paste it down I'm gonna say okay and then I'm + +01:09.100 --> 01:16.440 +gonna start it what does it do now it says I'm not doing it anymore on there + +01:16.440 --> 01:24.520 +goes live on YouTube it's yes live on YouTube it's alright we're there we're + +01:24.520 --> 01:29.080 +live on the YouTube as well so here we go ladies and gentlemen thank you very + +01:29.080 --> 01:33.560 +much for joining me let's do this thing peer tube should be up can you check + +01:33.560 --> 01:39.320 +that for me peer tube should be up we should be live there I see myself live + +01:39.320 --> 01:44.400 +there I should also be on rumble peer to good here we go ladies and gentlemen + +01:44.400 --> 01:47.120 +thanks for joining me + +02:14.400 --> 02:36.160 +thank you very much the latest data tells us that we're dealing with + +02:36.160 --> 02:39.160 +essentially a worst case + +02:56.560 --> 03:03.240 +what kind of link did you put in there that YouTube video what is that and enjoy + +03:03.240 --> 03:14.600 +skip to six minutes and enjoy what's that all about I'm curious what that is I + +03:14.600 --> 03:19.600 +think truth is good for kids we're so busy lying we don't even recognize the + +03:19.600 --> 03:24.240 +truth no more in this society we want everybody to feel good that's not that's + +03:24.240 --> 03:31.000 +not the way life is but you can tell if someone's lying you know you can sort of + +03:31.000 --> 03:36.360 +feel it in people and I have lied I'm sure I'll lie again I don't want to lie + +03:36.360 --> 03:40.000 +you know I don't think I'm a liar I try not to be a liar I don't want to be a + +03:40.000 --> 03:45.600 +liar I think it's like really important not to be a liar + +04:01.000 --> 04:31.000 +I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar + +04:31.000 --> 05:01.000 +I'm a liar I'm a liar I am a liar I am a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a modifier I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a liar I'm a + +05:01.000 --> 05:03.480 +Afternoon, I'm 20 minutes late again, + +05:03.480 --> 05:06.120 +but that seems to be the way it is. + +05:06.120 --> 05:09.960 +20 minutes late, but on time, so to speak. + +05:11.720 --> 05:13.320 +Business can go on biological. + +05:16.720 --> 05:19.080 +We are still trying to dispel the mythology + +05:19.080 --> 05:21.360 +that people are doubling and tripling down on, + +05:21.360 --> 05:23.240 +and we're trying to dispel that mythology, + +05:23.240 --> 05:25.240 +that enchantment with some knowledge. + +05:26.600 --> 05:29.160 +If you've been staying focused on the biology, + +05:29.160 --> 05:30.840 +you are not drowning in this way. + +05:31.720 --> 05:34.440 +You've been skillfully using TV and social media. + +05:34.440 --> 05:35.560 +You might be in big trouble, + +05:35.560 --> 05:37.280 +but if you stay focused on the biology, + +05:37.280 --> 05:39.680 +you don't take the pain on those systems, + +05:39.680 --> 05:41.720 +and you love your neighbor, you can escape. + +05:41.720 --> 05:42.720 +I do believe that. + +05:42.720 --> 05:44.560 +I do believe there's hope. + +05:44.560 --> 05:46.320 +And most of the hope comes from the fact + +05:46.320 --> 05:49.360 +that people are still sharing this work after four years. + +05:49.360 --> 05:52.720 +Still think that this is a viable message. + +05:52.720 --> 05:55.240 +You can find it at gigahomebiological.com, + +05:55.240 --> 06:00.200 +gigahome.bio, and stream.gigahome.bio. + +06:00.200 --> 06:04.400 +There are ways to support the links, share the links. + +06:04.400 --> 06:05.960 +We're trying to do our best to make sure + +06:05.960 --> 06:09.120 +that we can get it out as far as we can. + +06:09.120 --> 06:10.720 +My friends and friends next day. + +06:14.320 --> 06:16.960 +And so that's why I haven't done a sub stack + +06:16.960 --> 06:18.800 +in more than a week, which is not good, + +06:18.800 --> 06:20.440 +but I do have one translated. + +06:20.440 --> 06:23.720 +I just haven't done the transcript, + +06:23.720 --> 06:26.520 +which is, I admit, is frustrating. + +06:26.520 --> 06:28.000 +I also have an updated this list, + +06:28.000 --> 06:29.520 +which is also frustrating. + +06:30.360 --> 06:32.320 +But I have a lot of things that I'm working on + +06:32.320 --> 06:35.040 +that I think are more important than updating this list, + +06:35.040 --> 06:36.680 +even though I do appreciate it. + +06:36.680 --> 06:39.440 +We are going to update it soon. + +06:39.440 --> 06:41.120 +I really think there's work to be done. + +06:41.120 --> 06:42.720 +Everybody that's supporting this stream, + +06:42.720 --> 06:47.080 +I think, understands very well what we're up against. + +06:47.080 --> 06:49.840 +And so, if your name is not on this list + +06:49.840 --> 06:51.200 +and it should be, please forgive me. + +06:51.200 --> 06:54.200 +It will be there in a couple of days. + +06:54.200 --> 06:56.360 +And the list is slowly growing, + +06:56.360 --> 06:58.880 +but we could use always more subscribers. + +06:58.880 --> 07:01.200 +We're far lower than we need to be. + +07:01.200 --> 07:03.800 +If this is gonna be a real sustainable action, + +07:05.000 --> 07:06.600 +we can't run on fumes forever, + +07:06.600 --> 07:10.280 +but my wife and my family will do it as long as I can. + +07:10.280 --> 07:11.840 +This is the independent bright web. + +07:11.840 --> 07:15.080 +That means that we're not sponsored by anybody but you. + +07:15.080 --> 07:18.520 +We don't have, we're not selling lotion or soap + +07:18.520 --> 07:23.520 +or neutraceuticals, none of that stuff. + +07:24.280 --> 07:27.680 +We are just supported by people with kids, + +07:27.680 --> 07:29.200 +people who lost jobs, + +07:30.520 --> 07:34.040 +people who are trying to engage in United Noncompliance + +07:34.040 --> 07:38.560 +and establish the system, + +07:38.560 --> 07:41.520 +the network of people that can help you + +07:41.520 --> 07:43.480 +engage in United Noncompliance. + +07:43.480 --> 07:45.640 +I hope that's what GINGO and biological will be + +07:45.640 --> 07:49.200 +in the future is a network hub for people engaged + +07:49.200 --> 07:51.640 +in United Non Compliance. + +07:57.680 --> 08:04.680 +Good afternoon ladies and gentlemen, + +08:04.680 --> 08:07.680 +this is GINGO, biological and high resistance. + +08:07.680 --> 08:10.240 +Low noise information we've brought to you by a biologist. + +08:10.240 --> 08:12.200 +I'm coming to you live from Pittsburgh, Pennsylvania + +08:12.200 --> 08:13.280 +in the back of my garage. + +08:13.280 --> 08:16.560 +It is the 27th of March, 2024. + +08:16.560 --> 08:18.320 +I am a human just like you fighting + +08:18.320 --> 08:19.880 +for our grandchildren's future, + +08:19.880 --> 08:21.680 +our grandchildren's freedom, + +08:21.680 --> 08:23.920 +our grandchildren's informed consent. + +08:23.920 --> 08:25.360 +And I hope you are too. + +08:25.360 --> 08:27.360 +If you're joining me for the first time, + +08:27.360 --> 08:32.000 +GINGO and biological is a news source + +08:32.000 --> 08:34.600 +that is more than well aware of the idea + +08:34.600 --> 08:37.240 +that we've been consciously and intelligently manipulated + +08:37.240 --> 08:40.200 +in our habits and in our opinions for decades + +08:40.200 --> 08:42.560 +and that this has only increased in power + +08:42.560 --> 08:47.560 +and in its all encompassing application to our lives + +08:48.240 --> 08:50.880 +with the advent of the cell phone and the internet. + +08:52.200 --> 08:55.800 +These social media apps have been weaponized also + +08:55.840 --> 08:58.600 +from the perspective of who these people are + +08:58.600 --> 09:00.080 +and who is on them. + +09:00.080 --> 09:03.200 +You can see all kinds of people in this picture + +09:03.200 --> 09:07.240 +that I believe are very much in on this. + +09:07.240 --> 09:09.640 +You can look at the picture behind me + +09:09.640 --> 09:11.760 +and you can find lots of people + +09:11.760 --> 09:14.520 +who have wittingly and unwittingly contributed + +09:14.520 --> 09:19.120 +to this slow motion demolition of Western society + +09:19.120 --> 09:22.360 +and particular the jewel of Western society, + +09:22.360 --> 09:23.640 +the United States of America. + +09:23.640 --> 09:25.280 +And it's not because we do everything right + +09:25.400 --> 09:27.040 +because all of our teams are the best + +09:27.040 --> 09:30.560 +or because we win the gold medals. + +09:30.560 --> 09:33.000 +It's because our system is the one + +09:33.000 --> 09:34.960 +that has that inertia built into it + +09:34.960 --> 09:38.760 +around free speech and other protected freedoms. + +09:38.760 --> 09:43.120 +That inertia is what they need us to kind of discount + +09:43.120 --> 09:46.840 +as valuable and not be vigilant enough to guard + +09:46.840 --> 09:50.680 +and to eventually just give up for our children + +09:50.680 --> 09:55.000 +or not teach our children the level of vigilance + +09:55.000 --> 09:57.720 +that's necessary in order to preserve those freedoms. + +09:57.720 --> 09:58.880 +I think that's where we are. + +09:58.880 --> 10:01.440 +And they have been consciously manipulating our opinions + +10:01.440 --> 10:04.000 +about these freedoms for decades on purpose. + +10:04.000 --> 10:06.360 +And the last manipulation that occurred indeed + +10:06.360 --> 10:10.440 +was the declaration of a coronavirus pandemic + +10:10.440 --> 10:13.760 +wherein most of our understanding of public health + +10:13.760 --> 10:17.720 +was permanently shaken and we are now just basically + +10:17.720 --> 10:19.640 +under the influence of a mythology + +10:19.640 --> 10:21.400 +and those people who are willing + +10:21.400 --> 10:24.280 +to spectacularly commit to it. + +10:25.920 --> 10:26.960 +To the mythology. + +10:28.320 --> 10:29.680 +And that's why we haven't been able + +10:29.680 --> 10:31.200 +to exercise informed consent. + +10:31.200 --> 10:36.040 +That mythology is based around an ongoing RNA viral pandemic + +10:36.040 --> 10:39.320 +that has changed color, that has changed flavor + +10:39.320 --> 10:43.360 +and has changed flavor in a very uniform planetary manner. + +10:43.360 --> 10:46.360 +It is a phenomenon at a molecular level + +10:46.360 --> 10:49.720 +that we have never witnessed anything remotely close to it + +10:49.720 --> 10:50.760 +in the past. + +10:50.760 --> 10:54.320 +It is blamed on things as simple as a few bases + +10:54.320 --> 10:56.600 +or a few amino acids which encode + +10:56.600 --> 10:58.160 +an extra fear and cleavage site + +10:58.160 --> 11:00.360 +or an extra poly basic cleavage site. + +11:00.360 --> 11:04.600 +And with with those a few basic mythological assumptions + +11:04.600 --> 11:07.200 +inserted into the storyline carefully on the left + +11:07.200 --> 11:10.280 +and the right by actors that were agreed to disagree. + +11:10.280 --> 11:12.480 +We now believe that variants are trackable + +11:12.480 --> 11:14.000 +and that their evidence of evolution. + +11:14.000 --> 11:16.520 +We believe that lockdown would have done correctly + +11:16.520 --> 11:19.440 +prevents the spread of dangerous viruses like this one. + +11:19.440 --> 11:22.160 +And that we believe that without strictly regulations + +11:22.200 --> 11:25.200 +these kinds of gain of function things will happen again. + +11:25.200 --> 11:28.560 +We are being led to believe that this faith + +11:28.560 --> 11:30.440 +is something that we cannot question + +11:30.440 --> 11:33.440 +and we've been led to believe it by different narratives. + +11:33.440 --> 11:35.000 +Narratives were different in Australia + +11:35.000 --> 11:37.200 +than they were in Europe than they were in America. + +11:37.200 --> 11:40.240 +The number of participants that come out of the audience + +11:40.240 --> 11:43.560 +pretending to be innocent audience members + +11:43.560 --> 11:46.160 +when really they're participating in the illusion + +11:46.160 --> 11:48.440 +was different depending on what country you're in. + +11:48.440 --> 11:52.000 +But I in America, I assure you that we were involved + +11:52.000 --> 11:55.480 +in a magic show and audience members + +11:55.480 --> 11:57.920 +were pulled out of the audience and brought on stage. + +11:57.920 --> 12:00.120 +And we were made to believe that those people + +12:00.120 --> 12:02.120 +were innocently participating in the show + +12:02.120 --> 12:03.840 +when they were not. + +12:03.840 --> 12:05.440 +They were definitely not. + +12:08.440 --> 12:11.240 +And those same people now agree across the board + +12:11.240 --> 12:13.040 +that transfection isn't bad. + +12:13.040 --> 12:15.440 +Transfection probably saved millions of lives + +12:15.440 --> 12:18.100 +even if it also injured hundreds of thousands. + +12:19.320 --> 12:20.360 +That's where we are. + +12:21.360 --> 12:24.160 +And unfortunately, the faith in the novel virus + +12:24.160 --> 12:26.520 +going on for five years is now converting + +12:26.520 --> 12:28.400 +into a faith in a novel biology + +12:28.400 --> 12:30.680 +that again includes the biology of the virus, + +12:30.680 --> 12:32.760 +which is the fear and cleavage site + +12:32.760 --> 12:34.680 +as evidence of laboratory leak + +12:34.680 --> 12:39.160 +and then the complete unnecessary effort + +12:39.160 --> 12:42.920 +to track this thing all over the place + +12:42.920 --> 12:44.720 +but then not look at any epitopes + +12:44.720 --> 12:48.040 +that we were told to be afraid of from the very beginning. + +12:48.080 --> 12:51.360 +It's laughable to me that people who have claimed + +12:51.360 --> 12:52.720 +that the fear and cleavage site + +12:52.720 --> 12:57.280 +is a singular attribute of the virus + +12:57.280 --> 13:00.040 +which can contribute to both its infectivity + +13:00.040 --> 13:02.960 +and also its rapid spread around the world + +13:02.960 --> 13:05.480 +never bothered to keep track of it. + +13:05.480 --> 13:08.040 +Not even in one strain, not even commenting + +13:08.040 --> 13:10.960 +on whether the fear and cleavage site had optimized + +13:10.960 --> 13:12.640 +or disappeared in any of the strains + +13:12.640 --> 13:14.040 +and they still don't care. + +13:15.680 --> 13:17.840 +And if that doesn't strike you as odd, + +13:17.840 --> 13:19.080 +I don't know what to tell you + +13:19.080 --> 13:21.600 +because those same people are also convinced + +13:21.600 --> 13:23.840 +as they were in 2020, + +13:23.840 --> 13:25.840 +that there would be neurological side effects + +13:25.840 --> 13:29.160 +that at first started from the traveling of the virus + +13:29.160 --> 13:33.480 +up the olfactory bulb and into the brain directly + +13:33.480 --> 13:35.480 +but it also had to do with the spike protein + +13:35.480 --> 13:37.920 +and its ability to cause other proteins to fold + +13:37.920 --> 13:40.920 +by some heretofore, undescribed pre-onogenic + +13:40.920 --> 13:43.520 +or amyloidogenic mechanisms. + +13:43.520 --> 13:46.680 +These terms and these potentials + +13:46.680 --> 13:51.680 +were thrown around as often and as frequently as possible + +13:52.520 --> 13:55.240 +by a number of actors on the left and the right, + +13:55.240 --> 13:57.800 +on the front and the back in the mainstream media + +13:57.800 --> 14:00.480 +and on social media backwaters. + +14:00.480 --> 14:03.240 +And they were all united on these things very early + +14:03.240 --> 14:06.000 +which is odd because they're still central + +14:06.000 --> 14:08.360 +to the faith in this novel biology. + +14:09.240 --> 14:11.520 +We keep coming back to these same storylines + +14:11.520 --> 14:15.240 +that just don't have a basis in biology + +14:15.240 --> 14:18.320 +playing little clips where virologists complain + +14:18.320 --> 14:21.640 +about fear and cleavage sites is not sufficient evidence + +14:21.640 --> 14:25.240 +to explain how a few amino acids or a few bases + +14:25.240 --> 14:30.240 +in an mRNA, a modified RNA, a synthetic RNA spilled + +14:30.240 --> 14:33.360 +on the ground could result in pandemic coverage + +14:33.360 --> 14:37.680 +of that same molecule and insists that it has to do + +14:37.680 --> 14:41.200 +with this particular set of bases, it's absurd + +14:41.200 --> 14:43.400 +but they're still doing it now. + +14:43.400 --> 14:47.880 +The same fake people that claim to be really interested + +14:47.880 --> 14:50.040 +in finding the origin and really interested + +14:50.040 --> 14:53.400 +in catching the bad guys that are really interested + +14:53.400 --> 14:56.480 +in blaming eco-health alliance and the diffuse proposal. + +14:56.480 --> 15:00.200 +These clowns are the same clowns who were saying this stuff + +15:00.200 --> 15:02.440 +in 2020 with less evidence, + +15:04.040 --> 15:06.600 +with less basis for these speculations + +15:06.600 --> 15:08.800 +but they were just as certain back then. + +15:09.800 --> 15:13.800 +And there's still video evidence of these people + +15:13.800 --> 15:16.800 +and their certainty online freely available for you + +15:16.800 --> 15:19.800 +to go and check that these aren't new ideas. + +15:19.800 --> 15:21.800 +These are ideas that were planted very early + +15:21.800 --> 15:26.800 +by a coordinated obviously group of people loosely connected + +15:27.800 --> 15:29.800 +to a couple of weaponized piles of money. + +15:29.800 --> 15:31.800 +That's the best way I can describe it + +15:31.800 --> 15:35.800 +and if we don't break this faith in a novel virus + +15:35.800 --> 15:37.800 +that was probably a conflated background signal + +15:37.800 --> 15:42.800 +be it actual endosomes, exosomes, viruses, whatever + +15:43.800 --> 15:47.800 +or be it just genetic noise in the background + +15:47.800 --> 15:49.800 +as the cartoon up there just changed to. + +15:50.800 --> 15:53.800 +Either way, a noise signal in the background + +15:53.800 --> 15:55.800 +that we weren't detecting but could be detected + +15:55.800 --> 15:58.800 +that was then misconstrued as spread + +15:58.800 --> 16:02.800 +is a very parsimonious explanation for what really happened. + +16:03.800 --> 16:07.800 +It's funny because no one with any social media reach + +16:07.800 --> 16:10.800 +has ever even bothered to consider the possibility. + +16:10.800 --> 16:13.800 +That's the one possibility that Kevin McCurnan, + +16:13.800 --> 16:17.800 +Jessica Rose, John Bodewin, Steve Kirsch, + +16:17.800 --> 16:22.800 +Robert Malone, Robbie Kennedy Jr., Peter McCullough, + +16:22.800 --> 16:25.800 +Jay Bhattacharya, anybody that's ever talked to me + +16:25.800 --> 16:28.800 +when they hear me say that, that's the one thing that they, + +16:28.800 --> 16:31.800 +I don't know, I can't really understand that. + +16:31.800 --> 16:34.800 +I don't fully get it. You'll have to explain that. + +16:34.800 --> 16:36.800 +I can't really think about that hypothesis. + +16:36.800 --> 16:39.800 +That's hard for me. That's outside of my expertise. + +16:39.800 --> 16:42.800 +How many times have I heard that nonsense about clones? + +16:42.800 --> 16:45.800 +How about transfection and about previous + +16:45.800 --> 16:48.800 +and conflated background signals over and over again? + +16:48.800 --> 16:51.800 +These people who see me in person meet me at parties, + +16:51.800 --> 16:55.800 +take selfies with me and tell me how great they love my stream + +16:55.800 --> 16:59.800 +but they just can't verify or think about some of the ideas. + +16:59.800 --> 17:03.800 +They're really compelling, but it's not my expertise. + +17:03.800 --> 17:10.800 +They've had four years, just like I have, just like Mark has, + +17:10.800 --> 17:12.800 +just like George Webb has. + +17:12.800 --> 17:14.800 +We've all had four years and somehow or another, + +17:14.800 --> 17:18.800 +they still have their heads in the exact same holes. + +17:22.800 --> 17:23.800 +That's how you know that they're... + +17:23.800 --> 17:26.800 +Scientific questions, I think, are very hard to get a handle on. + +17:26.800 --> 17:30.800 +The reason for this is that in late modernity, + +17:30.800 --> 17:34.800 +which we're living in, there's simply too much knowledge + +17:34.800 --> 17:37.800 +for any individual human to understand all of it. + +17:37.800 --> 17:43.800 +In this world of extreme paper specialization + +17:43.800 --> 17:48.800 +where it's narrower and narrower subsets of experts policing themselves + +17:48.800 --> 17:50.800 +and talking about how great they are, + +17:50.800 --> 17:52.800 +the string theorists talking about how great string theory is, + +17:52.800 --> 17:55.800 +the cancer researchers talking about how they're just about to cure cancer, + +17:55.800 --> 17:59.800 +the quantum computer researchers are just about to build a quantum computer + +17:59.800 --> 18:01.800 +that will be a massive breakthrough. + +18:01.800 --> 18:04.800 +If you were to say that all these fields, not much is happening, + +18:04.800 --> 18:06.800 +people just don't have the authority for this. + +18:06.800 --> 18:11.800 +This is somehow a very different feel for science or knowledge + +18:11.800 --> 18:15.800 +than you would have had in 1800 or even in 1900. + +18:15.800 --> 18:18.800 +1800 Goethe could still understand just about everything. + +18:18.800 --> 18:23.800 +1900, Hilbert could still understand just about all of mathematics. + +18:23.800 --> 18:25.800 +And so the sort of... + +18:25.800 --> 18:27.800 +Oh, I'm in the wrong... + +18:27.800 --> 18:31.800 +I think it has made it a much harder question to get a handle on. + +18:31.800 --> 18:35.800 +So I would say that he is misleading the young with this presentation + +18:35.800 --> 18:38.800 +because he is calling knowledge that's not knowledge. + +18:38.800 --> 18:40.800 +It should be called noise. + +18:40.800 --> 18:43.800 +We have come to acknowledge those of us who understand + +18:43.800 --> 18:46.800 +what academic biology and science has become + +18:46.800 --> 18:51.800 +with the P-value hypothesis falsification thing + +18:51.800 --> 18:56.800 +from Paparian science has created a scenario where + +18:56.800 --> 19:03.800 +there is much more noise in our academic understanding of the world. + +19:03.800 --> 19:06.800 +And so he's suggesting that that noise is knowledge + +19:06.800 --> 19:10.800 +and it's just so specialized and so detailed + +19:10.800 --> 19:13.800 +that people can't be general scholars anymore. + +19:13.800 --> 19:16.800 +And that's absolutely not true. + +19:16.800 --> 19:20.800 +What he is suggesting is that young people don't bother to try. + +19:20.800 --> 19:25.800 +What he is suggesting is that young people don't bother to try. + +19:25.800 --> 19:28.800 +Now, with a little bit of work, + +19:28.800 --> 19:30.800 +I feel like that I've always been there. + +19:30.800 --> 19:34.800 +I've always been trying to integrate across fields as best I can + +19:34.800 --> 19:36.800 +as a biologist at heart. + +19:36.800 --> 19:41.800 +And so for me, this has always been the shortcoming of neuroscience. + +19:41.800 --> 19:45.800 +Is that the specialization makes it very hard for us to evaluate + +19:45.800 --> 19:47.800 +what each other is doing. + +19:47.800 --> 19:52.800 +And very hard to understand where the real cutting edge of understanding is. + +19:52.800 --> 19:55.800 +And it's almost by design. + +19:55.800 --> 19:58.800 +And I was frustrated by it, but I didn't know how to express that. + +19:58.800 --> 20:00.800 +I didn't know why I was frustrated. + +20:00.800 --> 20:04.800 +And I didn't know at the heart of it was this ritual of probability + +20:04.800 --> 20:09.800 +and falsification that created the illusion of progress. + +20:09.800 --> 20:15.800 +And so he's not saying that even though I assure you he understands it perfectly. + +20:16.800 --> 20:21.800 +He is signaling to some people and he is enchanting others. + +20:21.800 --> 20:23.800 +That's the beauty of this. + +20:24.800 --> 20:28.800 +Using the right code words, everybody that knows that he's the head + +20:28.800 --> 20:33.800 +of a giant weaponized pile of money and that he's the head of a information + +20:33.800 --> 20:39.800 +algorithmic mining DoD operation called Palantir. + +20:40.800 --> 20:43.800 +Then you know that he's not saying exactly what he means. + +20:44.800 --> 20:48.800 +But if you're just a casual listener to the portal podcast on its eighth episode + +20:48.800 --> 20:51.800 +or its second episode or its first episode. + +20:53.800 --> 20:56.800 +And then what you hear is somebody you think is telling the truth. + +20:58.800 --> 21:01.800 +But if he was telling you the truth, then he would have listed cancer + +21:01.800 --> 21:06.800 +and quantum computing and virology. + +21:07.800 --> 21:12.800 +And molecular biology and neuroscience. + +21:13.800 --> 21:17.800 +As places where it's very hard to tell how much progress is really being made. + +21:17.800 --> 21:21.800 +And in fact, in many of these fields, it may not be being made. + +21:22.800 --> 21:26.800 +So he listed a couple, but he didn't list them all. + +21:28.800 --> 21:30.800 +And so is that lying? + +21:30.800 --> 21:35.800 +But you can tell if someone's lying, you know, you can sort of feel it in people. + +21:36.800 --> 21:37.800 +And I have lied. + +21:37.800 --> 21:38.800 +I'm sure I'll lie again. + +21:38.800 --> 21:39.800 +I don't want to lie. + +21:39.800 --> 21:41.800 +You know, I don't think I'm a liar. + +21:41.800 --> 21:42.800 +I try not to be a liar. + +21:42.800 --> 21:43.800 +I don't want to be a liar. + +21:43.800 --> 21:46.800 +I think it's like really important not to be a liar. + +21:47.800 --> 21:51.800 +It's really important not to be a liar, says Tucker Carlson, the professional liar. + +21:51.800 --> 21:56.800 +The critical cut I have on the specialization is always that if you analyze + +21:56.800 --> 21:59.800 +the politics of science, the specialization should make you suspicious. + +22:00.800 --> 22:03.800 +It's because it's gotten harder to evaluate what's going on. + +22:03.800 --> 22:06.800 +And it's presumably gotten easier for people to lie and to exaggerate. + +22:07.800 --> 22:09.800 +And then one should be a little bit suspicious. + +22:09.800 --> 22:14.800 +So they are lying and exaggerating. + +22:14.800 --> 22:19.800 +Just like Peter Thiel says, they are lying and exaggerating about the fidelity of + +22:19.800 --> 22:24.800 +understanding in virology, the fidelity of understanding in cancer biology, + +22:24.800 --> 22:28.800 +the fidelity of understanding in the genetic causes of childhood diseases. + +22:28.800 --> 22:31.800 +They are feigning this. + +22:32.800 --> 22:36.800 +Every time they do a TED talk about what's going to happen in 10 years and about what + +22:36.800 --> 22:39.800 +the AI is going to do and about what climate change is going to happen, + +22:39.800 --> 22:41.800 +they are feigning this knowledge. + +22:41.800 --> 22:45.800 +They are feigning this certainty because the hyperspecialization of science + +22:45.800 --> 22:50.800 +and the use of reiterative, prepare and falsification of hypotheses has + +22:50.800 --> 22:55.800 +allowed the creation of this noise that is being misconstrued by people as + +22:55.800 --> 22:58.800 +powerful as Peter Thiel as knowledge. + +22:59.800 --> 23:04.800 +And if people as powerful as Peter Thiel and the puppets that he puts on the + +23:04.800 --> 23:08.800 +internet and promotes, like the Weinstein brothers are going to say that + +23:08.800 --> 23:12.800 +this guy is right, then we have an illusion of consensus. + +23:13.800 --> 23:16.800 +And that's what the intellectual dark web provided them. + +23:16.800 --> 23:20.800 +An illusion of consensus that could be used to govern, that could be used to + +23:20.800 --> 23:25.800 +influence the very opinions and habits of the masses. + +23:28.800 --> 23:32.800 +Just like Edward Bernays described, just like Noam Chomsky described a + +23:32.800 --> 23:36.800 +limited spectrum of debate within which there is a very lively discussion + +23:38.800 --> 23:42.800 +within which you can even encourage the most critical views + +23:43.800 --> 23:49.800 +because even the most critical views reinforce the presuppositions of the state. + +23:49.800 --> 23:53.800 +In this case, the presuppositions are the model of reality. + +23:55.800 --> 23:59.800 +The model of reality that they have been enforcing is one of lots of viruses, + +23:59.800 --> 24:03.800 +lots of pathogens, lots of dirty in the nature. + +24:05.800 --> 24:10.800 +And lots of things that you need as products to protect you from that, + +24:10.800 --> 24:15.800 +to detect that, to monitor that, to administer + +24:16.800 --> 24:18.800 +prophylactic for that. + +24:19.800 --> 24:26.800 +And it's a giant mythology created by our willingness to give over our attention + +24:27.800 --> 24:32.800 +and our trust to people like him and the applications that they sell us + +24:32.800 --> 24:33.800 +on our phones. + +24:36.800 --> 24:39.800 +So I wanted to go back to the video that I watched the other day because we + +24:39.800 --> 24:40.800 +didn't finish it yesterday. + +24:40.800 --> 24:42.800 +So this is, I'm just going to start it right away. + +24:42.800 --> 24:43.800 +Here we go. + +24:43.800 --> 24:47.800 +This is when Venner comes on stage and he talks about how we've, + +24:47.800 --> 24:51.800 +we've gone from understanding protein as a genetic material to DNA. + +24:51.800 --> 24:52.800 +It's a genetic material. + +24:52.800 --> 24:54.800 +I'm going to speed it up through that part. + +24:54.800 --> 24:57.800 +And then I'm going to set it back to somewhat normal when he gets done + +24:57.800 --> 24:59.800 +with the part that we saw yesterday. + +24:59.800 --> 25:00.800 +Thanks for joining me. + +25:02.800 --> 25:03.800 +Here for yourself as well. + +25:03.800 --> 25:06.800 +I'd like to welcome Craig Venter who's going to speak about what is life, + +25:06.800 --> 25:07.800 +a 21st century perspective. + +25:07.800 --> 25:08.800 +Craig. + +25:16.800 --> 25:18.800 +I see in the chat there that somebody's talking about. + +25:18.800 --> 25:19.800 +Thank you for that. + +25:19.800 --> 25:20.800 +We already heard this part. + +25:20.800 --> 25:25.800 +I can't stress enough how the power of AI is being used to distort what's + +25:25.800 --> 25:26.800 +actually going on. + +25:26.800 --> 25:31.800 +I'm going to watch a video tomorrow from 2017 where someone explains to us + +25:31.800 --> 25:33.800 +what's really happening on social media. + +25:33.800 --> 25:38.800 +It has nothing to do with, with neuronal networks and AI spontaneously + +25:38.800 --> 25:42.800 +becoming intelligent or, or algorithms that we don't really, + +25:42.800 --> 25:43.800 +I don't know. + +25:43.800 --> 25:44.800 +I don't know. + +25:44.800 --> 25:45.800 +I don't know. + +25:45.800 --> 25:46.800 +I don't know. + +25:46.800 --> 25:47.800 +I don't know. + +25:48.800 --> 25:51.800 +It's a simple, intelligent or, or algorithms that we don't really understand. + +25:51.800 --> 25:54.800 +It has to do with, as I've said in the last few days, very succinctly, + +25:54.800 --> 25:55.800 +it has to do with simple programming. + +25:55.800 --> 26:00.800 +If then else, that's it. + +26:00.800 --> 26:04.800 +Simple programming is enough to put Robert Malone at the top of the feed + +26:04.800 --> 26:07.800 +and put Jonathan Cooey on somebody else's feed. + +26:07.800 --> 26:11.800 +It's, it's simple programming is enough to make Jonathan Cooey think his + +26:11.800 --> 26:14.800 +message is getting out while everybody else doesn't see it. + +26:14.800 --> 26:20.980 +doesn't see it. It's as simple as that. It doesn't have to be a magic algorithm. You + +26:20.980 --> 26:27.920 +know what? Approving Remdesivir is as simple as saying that an AI chose it. And that AI + +26:27.920 --> 26:33.640 +could have been a simple one line code as Mark Housatonic has so eloquently said, if + +26:33.640 --> 26:43.120 +then else, Remdesivir. And so one of the bamboozledments, one of the greatest illusions that's currently + +26:43.120 --> 26:51.840 +being foisted upon us is the idea that AI is at the cusp of becoming more than just machine to + +26:51.840 --> 26:59.320 +control other people more than just a fancy machine. They want you to believe it's something + +26:59.320 --> 27:06.600 +that's going to have emergent properties very similar to life. And that's an illusion. That's a + +27:06.600 --> 27:12.480 +mythology. That's not going to happen. Can they use these algorithms and these, these, these + +27:12.600 --> 27:22.200 +software to control people to manipulate their opinions and their habits? Absolutely. But + +27:22.200 --> 27:31.120 +it's not gain a function in AI. It's not quantum computing that they were waiting for, or the + +27:31.120 --> 27:36.960 +big and a big enough computer, or a fast enough CPU. It's already happening right now with if + +27:36.960 --> 27:46.400 +then else statements, just programming the application to do it. And so when we talk about this, make + +27:46.400 --> 27:50.960 +sure you understand that part of this long term programming is getting people to think of + +27:50.960 --> 27:57.160 +themselves as a machine that the ghost in the machine doesn't matter that the sum of the whole + +27:57.160 --> 28:03.920 +doesn't matter. The whatever the pattern integrity becomes doesn't matter. We are, we are the + +28:03.920 --> 28:10.720 +Manila rope, we are the hemp rope, we are the nylon rope. That's what he wants us to believe chemistry + +28:10.720 --> 28:18.800 +and physics to be here for this event. And I was asked earlier whether the goal is to dissect what + +28:18.800 --> 28:24.480 +Schrodinger had spoken about in Britain, what to prevent, to present the new summary. And I always + +28:24.480 --> 28:29.640 +like to be forward looking. So I won't give you a history lesson except for very briefly. I will + +28:29.640 --> 28:34.320 +present our findings on first on reading the genetic code and then learning to synthesize and write + +28:34.320 --> 28:38.600 +the genetic code. And as many of you know, we synthesized an entire genome, booted it up to + +28:38.600 --> 28:44.720 +create an entirely new synthetic cell where every protein in the cell was based on the synthetic + +28:44.720 --> 28:50.120 +DNA code. So as you all know, Schrodinger's book was published in 1944. It was based on a series of + +28:50.120 --> 28:55.440 +three lectures here starting in February of 1943. And he had to repeat the lectures I read on the + +28:55.440 --> 28:59.960 +following Monday because the room on the other side of campus was too small. And I understand people + +28:59.960 --> 29:03.640 +were turned away tonight but were grateful for internet streaming so I don't have to do this + +29:03.640 --> 29:10.560 +twice. So also do clearly do his historical role and it's interesting to be sharing this event with + +29:10.560 --> 29:15.120 +Jim Watson who have known and had multiple interactions with over the last 25 years and including most + +29:15.120 --> 29:19.080 +recently sharing the double helix prize for human genome sequencing with him from Cold Spring Harbor + +29:19.080 --> 29:25.320 +laboratory a few years ago. Now Schrodinger started his lecture with a key question in an + +29:25.320 --> 29:29.040 +interesting insight on it. The question was I'll read it. How can the events in space and time which + +29:29.040 --> 29:33.000 +take place within the boundaries of a living organism be accounted for by physics and chemistry? + +29:33.000 --> 29:36.880 +It's a pretty straightforward, disembow question. And then he sort of answered what he could at the + +29:36.880 --> 29:41.160 +time. The obvious inability of present day physics and chemistry to account for such events is no + +29:41.160 --> 29:47.400 +reason at all for doubting that they will be accounted for by those sciences. So while I only have around + +29:47.400 --> 29:52.120 +40 minutes not three lectures I hope to convince you that there has been substantial progress in the + +29:52.120 --> 29:57.240 +last nearly 70 years since Schrodinger initially asked that question to the point where the answer is + +29:57.240 --> 30:03.360 +at least nearly at hand if not in hand. So I view that we're now in what I'm calling the digital age + +30:03.360 --> 30:09.920 +of biology. My teams work on synthesizing genomes based on digital code in the computer and four + +30:09.920 --> 30:14.520 +bottles of chemicals illustrates the ultimate link between the computer code and the digital code. + +30:15.240 --> 30:20.120 +Life is code as you heard in the introduction. It was very clearly articulated by Schrodinger. + +30:21.320 --> 30:26.040 +I know he's already starting out with a simplification that I didn't plug yesterday but I will now + +30:26.680 --> 30:35.720 +in that the DNA and the four bases can be translated into a computer code and digitized and then we + +30:35.720 --> 30:41.320 +have essentially taken that data and transferred it to another medium. What does that assume? + +30:42.280 --> 30:47.880 +It assumes a lot of things. First of all it assumes that any proteins or RNA that are + +30:47.880 --> 30:54.600 +associated with that DNA molecule are completely irrelevant and contain no information at all. + +30:55.480 --> 31:02.440 +It also suggests that the methylation state of the DNA doesn't matter at all and contains no + +31:02.440 --> 31:09.800 +information relevant to understanding its function. Now we could go on and on and on and on and on + +31:10.360 --> 31:15.720 +about all the things that's saying that taking DNA in the four bases and turning it into computer + +31:15.720 --> 31:21.480 +code is digitizing that information. Even if you can take that and make synthetic copy of it and + +31:21.480 --> 31:27.000 +put it back into a cell structure and get something resembling cell division to occur + +31:28.680 --> 31:36.120 +still does not say that you understand how that DNA works after you put it into that context that + +31:36.120 --> 31:42.920 +you can no longer probe. They don't have a bunch of cameras inside of their synthetic cell to + +31:42.920 --> 31:48.120 +see that everything is functioning and working the way that they think it does and that the DNA + +31:48.120 --> 31:54.680 +is not being chemically altered or that proteins and chaperone proteins and facilitating proteins + +31:54.680 --> 32:00.440 +and enzymes and RNA are not binding to that DNA and causing it to integrate back with that + +32:00.440 --> 32:08.920 +machinery to work properly. And so there are an extraordinary number of assumptions that are + +32:08.920 --> 32:15.000 +being made in order to simplify our own understanding of this irreducible complexity to make it seem + +32:15.000 --> 32:21.080 +like it's pretty it's not that complex at all. Code script. I think perhaps even more importantly + +32:21.080 --> 32:26.120 +and something I missed on the first few readings of his book early in my career was as far as I + +32:26.200 --> 32:31.800 +can tell it's the first mention that this code could be as simple as a binary code and he used + +32:31.800 --> 32:37.400 +the example of Morris code with just dots and dashes could be sufficient to give 34 different + +32:37.400 --> 32:44.440 +specifications. I've searched and I have not find any earlier references to the Morris code although + +32:44.440 --> 32:50.920 +historian that I know wrote a quick letter asking about that then and Chris response was + +32:50.920 --> 32:53.800 +it was a metaphor that was obvious to everybody. So I don't know if it was obvious to everybody + +32:53.800 --> 32:59.240 +after Schrodinger's book or sometime before. One of the things though Schrodinger was right + +32:59.240 --> 33:03.320 +about a lot of things which is why in fact I think we celebrate what he talked about and what he + +33:03.320 --> 33:09.400 +wrote about but some things he was clearly wrong about like most scientists in his time and he + +33:09.400 --> 33:14.840 +relied on the biologist of the day. They thought that protein not DNA was the genetic information + +33:16.120 --> 33:21.720 +and I think it's really quite extraordinary because just in 1944 in the same year that he + +33:21.720 --> 33:27.400 +published his book is when the famous experiment by Oswald Avery who was actually 65 and about + +33:27.400 --> 33:32.360 +ready to retire along with his colleagues Colin McCloud and McLean McCartney published their + +33:32.360 --> 33:39.240 +key paper demonstrating that DNA was actually the Colin McCloud of the clan McCloud is that the + +33:39.240 --> 33:44.600 +the immortal in Highlander one of the best movies of all time in case you haven't seen it Highlander + +33:44.600 --> 33:48.360 +great movie substance that causes bacterial transformation and therefore was the genetic + +33:48.360 --> 33:55.080 +material. His experiment was remarkably simple and I sort of wonder why it wasn't done 50 years + +33:55.080 --> 33:59.240 +earlier because with all the wonderful genetics work going on in Drosophila certainly people were + +33:59.240 --> 34:03.640 +looking at chromosomes. He simply used proteolytic enzymes to destroy all the proteins associated + +34:03.640 --> 34:08.440 +with the DNA and showed that the DNA the naked DNA was in fact of the transforming factor. + +34:09.800 --> 34:15.560 +The impact of this paper was far from instantaneous as has happened in this field I think in part + +34:15.560 --> 34:20.920 +because there was so much bias against DNA and towards proteins that it took a long time + +34:20.920 --> 34:26.120 +for that to sink. Now if you were paying attention there he's citing a paper that that tried to + +34:26.120 --> 34:31.240 +address the issue that I just said earlier which is if you just take that DNA and you don't have + +34:31.240 --> 34:35.320 +any chemical information about it you don't have any of the chaperone proteins or proteins or + +34:35.320 --> 34:40.920 +RNA that bound to it in in vivo then how do you know what you're really getting out of there and + +34:41.080 --> 34:46.280 +to a certain extent that experiment is valid in the sense of that the DNA transferred from + +34:46.280 --> 34:53.960 +one bacteria to another transferred those genes and those traits and so I'm totally willing to + +34:53.960 --> 35:02.040 +accept that some of these experiments are real and demonstrate that DNA is a genetic material that + +35:02.040 --> 35:08.680 +can carry a code that can be translated into proteins. I'm not suggesting that those kinds of broad + +35:08.680 --> 35:15.320 +ideas are incorrect. Please don't misunderstand me what I am suggesting though is that as they + +35:15.320 --> 35:19.560 +are described they are described in a very cartoonishly simple way + +35:22.840 --> 35:28.680 +and that cartoonishly simple thing involves DNA to RNA to protein with some ribosome in the middle. + +35:29.000 --> 35:37.880 +Not thinking about okay for example when we read RNA don't we isn't that basically this a very + +35:40.760 --> 35:46.600 +there are two steps there right understanding how those work in different tissues in different + +35:46.600 --> 35:53.320 +species with different ribosomes in different enzymes in different nucleic nuclear enzymes + +35:53.320 --> 36:00.600 +and nuclear chaperone proteins and and the variable list is endless and we're not talking + +36:00.600 --> 36:04.920 +about that variable list at all we're trying to ignore it we're doing as much reductionist + +36:05.960 --> 36:11.160 +discussion as we can about DNA here and reductionist experiments are important + +36:13.160 --> 36:20.760 +but not exhaustive and certainly not the experiments that are going to get across + +36:20.760 --> 36:27.480 +get us across the finish line of trying to crack this irreducible complexity. Ken in 1949 + +36:27.480 --> 36:33.960 +obviously was the first sequencing of a protein by Fred Sanger and the protein was insulin and + +36:33.960 --> 36:40.520 +this work showed in fact that proteins consisted of linear amino acid codes so and he won the Nobel + +36:40.520 --> 36:46.600 +Prize in 1958 for that achievement and was very key in terms of leader understanding of the link + +36:46.600 --> 36:51.560 +between DNA and proteins but as you heard in the introduction obviously the discovery that + +36:51.560 --> 36:59.080 +changed the whole field and started us down to DNA root was the 1953 work by Watson and Crick + +36:59.080 --> 37:03.560 +with help from Maurice Wilkins and Roslyn Franklin showing that DNA was in fact a double helix + +37:03.560 --> 37:08.760 +and had a clear explanation of how it could be self-replicated. Again this was not as I + +37:08.760 --> 37:14.440 +understand instantly perceived as a breakthrough because the biochemists were still hanging on + +37:14.440 --> 37:21.000 +to genetic code but soon with a few more experiments from others the world changed + +37:21.000 --> 37:25.640 +pretty dramatically. I think the next big thing came from the work obviously of Carana and Marshall + +37:25.640 --> 37:31.480 +Nuremberg in 1961 where they worked out the triplet genetic codes of three letters of genetic + +37:31.480 --> 37:37.080 +code coding for a single amino acid and then this became clear how the linear DNA code encoded for + +37:37.080 --> 37:42.520 +the linear protein code. This was followed a few years later by Robert Holly's discovery of the + +37:42.520 --> 37:48.440 +structure of tRNA and tRNA is the key the key a link between the messenger RNA and bringing the + +37:48.440 --> 37:54.840 +amino acids in for protein synthesis though Holly Nuremberg and Carana shared the Nobel Prize in 1968 + +37:54.840 --> 37:59.320 +for that work. The next decade brought us restriction enzymes so my friend and colleague Ham Smith + +37:59.320 --> 38:04.360 +who was at the Venture Institute now in 1970 that found the first restriction enzymes these are + +38:04.360 --> 38:09.640 +the molecular scissors that cut DNA very precisely and enabled the entire field of a molecular + +38:09.640 --> 38:13.560 +engineering and molecular biology and Ham Smith and Warner Arbor and Dan Nathan shared the prize + +38:13.560 --> 38:19.240 +in 1978 for that work. The 70s brought not only some interesting dress codes and characters + +38:20.440 --> 38:25.720 +but the beginning of the molecular splicing revolution using restriction enzymes Conan Boyer + +38:25.720 --> 38:31.800 +and Paul Berg published the first papers on recombinant DNA and Conan Boyer at Stanford + +38:31.800 --> 38:36.520 +as Stanford father patent on their work and this was used by Genentech and Eli Lilly to produce + +38:36.520 --> 38:43.800 +a human insulin as the first recombinant drug. DNA sequencing and reading the genetic code + +38:43.800 --> 38:50.360 +progressed much more slowly so in 1973 a maximum Gilbert published a paper on 24 base pairs 24 + +38:50.360 --> 38:56.920 +letters of genetic code RNA sequencing progressed a little bit faster so the first actual genome + +38:56.920 --> 39:03.000 +viral genome an RNA viral genome was sequenced in 1976 by Walter Fier is it from Belgium and this + +39:03.080 --> 39:08.280 +was followed the following year by Fred Sanger again a sequence in the first DNA virus and this was + +39:08.280 --> 39:15.640 +phi X 174 that I'll bring back to mind with you so this became the first DNA virus in the first + +39:15.640 --> 39:20.040 +viral DNA genome and it was also accompanied by this new sequencing technique called now referring + +39:20.040 --> 39:25.720 +to a Sanger sequencing that Sanger introduced. This is a picture of Sanger's team that sequenced + +39:25.720 --> 39:30.760 +phi X the second guy from the left on the bottom is Clyde Hutchinson who was also a member of the + +39:30.760 --> 39:36.120 +Adventure Institute and joined us after retiring from the University of North Carolina and took + +39:36.120 --> 39:41.960 +played a key role in some of the synthetic genome work. So in 1975 I was just getting my Ph.D. as + +39:41.960 --> 39:47.080 +the first genes were being sequenced 20 years later I led the team to sequence the first genome + +39:47.080 --> 39:52.040 +of a living species and Cam Smith was a key part of that team this was homophilus influenza + +39:52.040 --> 39:56.520 +instead of 5000 letters of genetic code this was 1.8 million letters of genetic code + +39:56.600 --> 40:02.600 +or about 300 times the size of the phi X genome. Five years later we up the ante another 1,600 + +40:02.600 --> 40:07.800 +times with the first draft of the human genome using these new whole genome shotgun techniques + +40:07.800 --> 40:12.920 +and the smaller papers the the public genome effort size doesn't reflect its significance + +40:12.920 --> 40:17.720 +only its relative significance to me. So the human genome is about a half a million times + +40:17.720 --> 40:22.440 +larger than the phi X genome so it shows how fast things were developing and reading genomes is + +40:22.440 --> 40:27.320 +now progressed extremely rapidly so instead of years or decades it now takes about two hours + +40:27.320 --> 40:33.240 +to sequence a human genome instead of genomes per day or genomes per hour or hours per genome + +40:33.240 --> 40:37.320 +we can now in a recently done a demonstration sequencing 2000 complete microbial genomes + +40:37.320 --> 40:45.320 +in one run in one hour so the pace is changing quite substantially. Now I view DNA as an analog + +40:45.320 --> 40:50.120 +coding molecule and when we sequence the DNA we're converting that analog code into digital code + +40:50.840 --> 40:56.680 +the ones and zeros in the computer very similar to the dots and dashes that Schrodinger + +40:56.680 --> 41:00.280 +indicated might be the original code and I call this process digitizing biology. + +41:01.080 --> 41:06.520 +A numerous scientists have drawn the analogy between computers and biology I take these even + +41:06.520 --> 41:11.560 +further I describe DNA as the software of life and when we activate a synthetic genome in a cell + +41:11.560 --> 41:15.880 +we call we describe it as booting up a genome in a cell the same way we talk about booting up + +41:15.960 --> 41:20.280 +software. Now June 23rd of this year was Alan Turing's would have been his hundredth birthday + +41:21.080 --> 41:26.040 +and Turing described what has become be known as Turing machines and the machine described a + +41:26.040 --> 41:30.600 +set of instructions written on a tape. He also described the universal Turing machine which was a + +41:30.600 --> 41:37.240 +machine that could take that set of instructions. I hope you can already feel how inadequate this is + +41:38.040 --> 41:46.440 +that our existence is really a machine that reads a tape. I hope you can understand how + +41:46.440 --> 41:51.880 +ridiculous it is that he is trying to convince this audience after years of failure that they + +41:51.880 --> 41:58.520 +are almost there to understanding the machine that reads the tape. Computers equal life, + +41:58.600 --> 42:09.320 +computers equal biology is one of the most absurd insults to God's creation one of the most absurd + +42:09.320 --> 42:16.760 +insults and it's a verbal abomination of the beauty of life. But that's what we're doing here + +42:16.760 --> 42:22.040 +right it's it's chemistry and physics which essentially means it's a bunch of zeros and ones + +42:22.040 --> 42:27.640 +it's a bunch of variables that if we had sufficient ability to measure them we could predict all of + +42:27.720 --> 42:31.960 +them. It essentially means we have no free will because we're just machines. + +42:33.560 --> 42:36.040 +The free will is an illusion. Do you see where this goes? + +42:38.360 --> 42:46.120 +This is from years ago this is one of the granddaddies of this mythology. This guy has been claiming + +42:46.120 --> 42:53.240 +that if we just had enough sequences like every baby on earth we would have a chance at figuring + +42:53.240 --> 42:58.040 +out how the genome works. This guy says that. This guy's got a foundation that has that as a goal. + +42:59.000 --> 43:07.240 +They charge you to sequence you and then they also use your sequence and sell it to pharmaceutical + +43:07.240 --> 43:15.640 +companies. That's his company. This is one of the darkest players in this mythology that understands + +43:15.640 --> 43:23.160 +that the value of the people on the planet right now is is there what they represent as data. + +43:24.120 --> 43:29.800 +And rewrite them and this was the original origin of the digital computer. His ideas were + +43:29.800 --> 43:36.040 +carried further in the 1940s by John Van Neumann as many people know and he conceived of the self + +43:36.040 --> 43:41.000 +replicating machine. So Von Neumann's machine consisted of a series of cells which encoded a + +43:41.000 --> 43:45.320 +sequence of actions that be performed by the machine and using a writing head the machine can print + +43:45.320 --> 43:50.840 +out a new pattern of cells allowing it to make a complete copy of itself on the tape. So many + +43:50.920 --> 43:55.800 +of people have certainly made the analogy most recently in nature Sydney Brenner who played a role + +43:55.800 --> 44:01.640 +in almost all these stages of early biology wrote an article about Turing and biology and in this + +44:01.640 --> 44:06.600 +he argued that the best examples of a Turing and von Neumann machine is found in biology with the + +44:06.600 --> 44:13.400 +self replicating code the internal description of itself and that this is the key kernel of + +44:13.400 --> 44:18.840 +biological theory. So while software was pouring out of sequencing machines around the world + +44:18.840 --> 44:25.000 +substantial progress was going on describing the hardware of life or proteins. So in biochemistry + +44:25.000 --> 44:29.880 +the first two decades of the 20th century were dominated by what was called the colloid theory. + +44:29.880 --> 44:34.120 +So life itself is explained in terms of the aggregate properties of all the colloidal substances + +44:34.120 --> 44:38.920 +in an organism. We now know that these substances are a collection of three-dimensional protein + +44:38.920 --> 44:45.320 +machines each evolved to carry out a very specific task. Now these proteins have been + +44:45.320 --> 44:50.840 +described as nature robots and if you think about it for every single task in a cell every + +44:50.840 --> 44:55.480 +imaginable task is described by a Tanford and Reynolds there is a unique protein to carry out + +44:55.480 --> 45:01.720 +that task. It's programmed when to go on when to go off and it does this based on its structure + +45:01.720 --> 45:07.160 +it doesn't have consciousness it doesn't have a control from a mind or a higher center everything + +45:07.160 --> 45:14.280 +a protein does is built in to its linear code derived from the DNA code. Just so that's a pretty + +45:14.360 --> 45:21.240 +bold bold certainty there with no room for subtleties that I hope he's going to build in later. + +45:21.240 --> 45:24.920 +Briefly there's multiple protein types everything you know about your own life + +45:24.920 --> 45:29.080 +most of it is protein derived about a quarter of your body is collagen it's a matrix protein + +45:29.080 --> 45:34.200 +that's built up in multiple layers we have rubber-like proteins that form blood vessels in the lung + +45:34.200 --> 45:39.160 +we have transporters that move things in and out of cells enzymes that copy DNA metabolized sugars + +45:39.160 --> 45:45.960 +etc. Now the most important breakthrough I think outside of the genetic code is determining the + +45:45.960 --> 45:50.520 +process of protein synthesis. To show you how recent all of this is this is the three-dimensional + +45:50.520 --> 45:58.360 +structure of the bacterial ribosome determined in 2005 and the three angstrom structure of the + +45:58.360 --> 46:01.880 +eukaryotic chromosome was just determined and published in December of last year that this is + +46:01.880 --> 46:08.120 +all recent science in recent history and these are extraordinary molecules but that's a weird + +46:08.120 --> 46:12.760 +thing right I mean if we sequence the whole human genome already then why do we wait so long + +46:12.760 --> 46:22.360 +to sequence a bacterial ribosome what did he say exactly because it doesn't make sense we sequence + +46:22.360 --> 46:29.640 +the whole human genome two decades ago already this is this is the three-dimensional structure + +46:29.640 --> 46:37.720 +of the bacterial ribosome determined in 2005 and the three angstrom structure of the eukaryotic + +46:37.880 --> 46:42.520 +weird because 2005 was one of the first I think it's one of the first years where we started to + +46:42.520 --> 46:48.760 +actually get coronavirus sequences did you know that the first human coronavirus that was actually + +46:48.760 --> 46:50.200 +sequenced and understood + +46:53.000 --> 46:57.960 +around 2005-2006 what a weird time for all these narratives to start right + +46:59.240 --> 47:04.920 +all together so it was just determined and published in December of last year that this is all + +47:04.920 --> 47:09.160 +weir of the eukaryotic chromosome was just determined and published in December and the + +47:09.160 --> 47:13.640 +three angstrom structure of the eukaryotic chromosome was just determined and published in December of + +47:13.640 --> 47:17.960 +last year is that the bacteria chromosome then I don't know what he's talking about there but + +47:17.960 --> 47:23.240 +it seems really weird this is all recent science in recent history and these are extraordinary + +47:23.240 --> 47:30.360 +molecules there it's the most complex machinery we have in the cell as you can see + +47:31.160 --> 47:36.920 +there's numerous components to it I tried to think of this as maybe the Ferrari engine of the cell + +47:38.040 --> 47:42.040 +if the engine can't convert the enmester RNA tape into proteins there is no life + +47:42.760 --> 47:47.000 +and if you interfere with that process you kill life so major antibiotics that we all know about + +47:47.000 --> 47:52.360 +the immunoglycosides, ceterocycline, quorum phenylchol, erythromycin, etc all kill bacterial cells + +47:52.360 --> 47:57.320 +by interfering with the function of this structure how many people knew that how many + +47:57.400 --> 48:05.800 +people knew that a ribosome was composed mostly of RNA so ribo proteins ribonucleic proteins + +48:06.520 --> 48:13.000 +and that it had 47 different proteins subunits or whatever that come together to form it including + +48:13.000 --> 48:19.400 +the RNA molecules that are hybrid in there does that sound like something that we understand + +48:22.040 --> 48:25.880 +or does that sound like something that they desperately want to understand if they don't + +48:25.960 --> 48:33.800 +understand that machine then what do they really understand about DNA to RNA to protein not very + +48:33.800 --> 48:42.360 +much think back to the violin analogy from the last couple days if they can explain to you why + +48:42.360 --> 48:48.200 +a crack in the neck can be absolutely catastrophic for a strativarius violin do they understand anything + +48:48.200 --> 48:53.960 +about the varnish process or about how a perfect violin works or how a violinist produces the music + +48:54.040 --> 49:01.560 +on a violin they haven't even bothered to study it and so by sequencing the human genome using + +49:01.560 --> 49:10.120 +restriction enzyme maps they haven't even started actually studying it by sequencing or crystallizing + +49:10.120 --> 49:18.280 +the bacterial ribosome we're just getting started studying it in 2005 at the same time where + +49:18.920 --> 49:24.600 +we're just starting to study coronavirus biology and and and able to find them in the wild + +49:26.040 --> 49:30.440 +and now we're supposed to believe that this is basically subject matter that is mastered + +49:31.640 --> 49:39.960 +that we can feed mRNA into a child's body through the muscle and expect ribosomes in their tissue + +49:40.520 --> 49:46.680 +to faithfully translate the protein that we want them to translate and for their immune system + +49:46.680 --> 49:54.040 +to faithfully respond to it with a useful response that's the model of reality that these guys + +49:54.040 --> 50:01.720 +want us to accept right now that the that the original story line was written for and laid down + +50:01.720 --> 50:11.080 +by charlatans like this man who are telling the same mythology the same nonsense story about how + +50:11.080 --> 50:18.920 +eventually we're going to be able to do all this stuff + +50:20.920 --> 50:25.800 +so the ribosome is clearly the most unique and special structure in the cell it has seven major + +50:25.800 --> 50:31.480 +RNA chains including three messenger three tRNA chains and one messenger RNA it has 47 different + +50:31.480 --> 50:36.760 +proteins going into the structure in one newly synthesized protein chain the size is several + +50:36.760 --> 50:40.920 +million dolphins so this is the heart of all biology we would not have cells we would not have + +50:40.920 --> 50:45.480 +life without this working and this is the machine that converts the linear dna code + +50:46.520 --> 50:52.200 +into proteins and the process with doing this what did he just say i mean think about how he said it + +50:52.200 --> 50:56.920 +so from there's a several million dolphins so this is the heart of all biology we would not have + +50:56.920 --> 51:02.360 +cells we would not have life without this working this is the heart of biology they don't have a + +51:02.360 --> 51:07.480 +clue how it works they've been telling you for years the heart of biology is dna it's the tape + +51:08.280 --> 51:13.880 +the tape that goes into the machine it's not the tape that goes into the machine at all + +51:17.400 --> 51:25.160 +it's the machine or the machines they don't know how the dna copying machine works they don't know + +51:25.160 --> 51:30.360 +how the RNA polymerase works they don't know how reverse transcriptase works they don't know how + +51:30.360 --> 51:35.640 +a ribosome works they can tell you they know but they don't + +51:41.480 --> 51:43.160 +do you see what we're dealing with here + +51:46.040 --> 51:48.200 +they can talk out of both sides of their mouths + +51:51.000 --> 51:56.600 +these people know that they haven't made progress just like peter teal told you in that video in + +51:57.160 --> 52:04.200 +2019 they know that the progress is stalling out that yeah we can sequence things faster we can + +52:04.200 --> 52:14.120 +sequence more things in less time we can identify cracks in the neck of the violin faster we can + +52:14.120 --> 52:19.240 +find them easier we have technology to locate hairline cracks that we're hard to see before + +52:19.960 --> 52:24.360 +does that mean that we understand how the violin makes music no we don't + +52:26.120 --> 52:34.200 +no we don't and these kinds of observations are being misconstrued as understanding they're not + +52:34.200 --> 52:44.600 +understanding please see it this is the biology the big picture biology we need to teach to our kids + +52:45.400 --> 52:49.400 +we are being misled by a bunch of people who have been misled + +52:51.160 --> 52:53.640 +who are too naive or too smart to see out of it + +52:59.880 --> 53:06.200 +and this is the machine that converts the linear dna code into proteins and the process with doing + +53:06.200 --> 53:11.640 +this RNA synthesis from RNA is called transcription and protein synthesis is called translation + +53:12.600 --> 53:17.160 +so if these processes were highly reliable life would be very different and perhaps not need the + +53:17.160 --> 53:21.160 +same kind of information driven system if you were building a factory to build automobiles + +53:21.160 --> 53:25.720 +that worked the way the ribosome did you would be out of business very quickly a significant + +53:25.720 --> 53:30.120 +fraction of all the proteins synthesized get degraded shortly after synthesis because they + +53:30.120 --> 53:34.920 +formed the wrong confirmations they aggregate in the cell or cause another problem so as + +53:34.920 --> 53:39.400 +transfer RNA brings in the final amino acid to a growing peptide chain coming out of the ribosome + +53:39.400 --> 53:43.080 +the next step is truly one of the most remarkable and that's the protein folding + +53:43.080 --> 53:48.360 +so the number of protein confirmations if you have 100 amino acids is only a word of 2 to the 100th + +53:48.360 --> 53:55.400 +power 2 to the 100th power if you have 100 amino acids so tell me again how we understand that + +53:55.400 --> 54:02.440 +preons can cause other proteins to misfold how does that work exactly why do we believe that + +54:02.440 --> 54:10.440 +that seems like a good idea like that makes sense proteins that have countless configurations + +54:10.440 --> 54:17.560 +that are complex electrostatic magnets with three dimensional shapes inside of a polar solvent + +54:17.560 --> 54:26.760 +called water and yet somehow or another despite that absolutely irreducible complexity this old + +54:26.760 --> 54:30.360 +man is still trying to tell us that we basically have a grip on how all that works + +54:32.120 --> 54:42.120 +or is he or is he listen the next step is truly one of the most remarkable + +54:42.120 --> 54:46.600 +and that's the protein folding so one of the most remarkable that we still don't understand but + +54:46.600 --> 54:53.080 +google tells us we do it's too bad that google fold can't explain the simple mechanisms by which + +54:53.160 --> 54:59.400 +proteins go into prion states or go into amyloidosis and cause other proteins to do it + +55:01.960 --> 55:07.240 +if the way that a protein folds is dependent on its sequence then how can a prion protein + +55:07.880 --> 55:12.200 +influence another protein without the right sequence to fold into that protein + +55:14.040 --> 55:18.520 +how come we don't talk about any of this stuff in the prion literature we just talk about in very + +55:18.520 --> 55:32.120 +grand terms he's telling you that proteins misfold all the time that if you started a car + +55:32.120 --> 55:38.840 +manufacturing plant that made cars as well as a ribosome does that you'd go out of business + +55:39.960 --> 55:46.200 +think about how extraordinary what he's saying is that must mean that there's a lot of misfolded + +55:46.280 --> 55:50.600 +protein in every cell that we have that must mean that misfolded protein must be dealt with pretty + +55:50.600 --> 55:56.680 +effectively but how do they know that it's a misfolded protein after it gets translated from + +55:56.680 --> 56:03.800 +DNA to RNA to protein who's checking what what mechanism is checking to see if that protein has + +56:03.800 --> 56:10.600 +all the right amino acids do they line it up against a standard i thought these things were + +56:10.600 --> 56:16.440 +just molecules with no mind yet somehow or another you're just going to keep hand waving + +56:16.440 --> 56:20.440 +over and over this happens and then that happens and then this happens and then they do this and + +56:20.440 --> 56:25.800 +then they do this and the most amazing thing is despite all of these errors and terrible folding + +56:25.800 --> 56:32.520 +and and the last transfer RNA being wrong the body is able to get rid of those proteins very + +56:32.520 --> 56:37.080 +quickly if they're folded wrong or if they're translated wrong because there's all these magic + +56:37.080 --> 56:49.000 +mechanisms that do it he is trying to make you believe that protein misfolding is a very normal + +56:49.000 --> 56:54.760 +thing it happens all the time it's something we have to deal with and can be pathogenic if it goes + +56:54.760 --> 57:05.880 +wrong is it true that's the question you have to ask yourself is it a natural process or is it + +57:05.880 --> 57:11.800 +something that happens after a long exposure to a toxin repeated exposure to chronic inflammation + +57:12.840 --> 57:17.800 +other insults to the brain or tissues which go through this protein misfolding process + +57:17.800 --> 57:27.000 +electromagnetic radiation or other damaging sources of biodamaged nobody talks about any of + +57:27.000 --> 57:33.480 +that stuff here and the reason why is because they are oversimplifying your understanding of + +57:33.480 --> 57:38.280 +what is irreducible complexity in biology the number of protein confirmations if you have 100 + +57:38.280 --> 57:43.560 +amino acids is on the order of two to the 100th power it would take about 10 to the 10th years + +57:43.560 --> 57:49.080 +that for you to even try all those if you can try one a second so wait i thought that google + +57:49.080 --> 57:54.360 +already has a algorithm that figured it all out and can do it for any protein do you see where we are + +57:55.720 --> 58:02.840 +did we figure that out in like 10 years just hardcore number crunching but built into this + +58:02.920 --> 58:06.520 +linear code of the amino acid based on the linear genetic code these processes + +58:07.080 --> 58:12.840 +happened very quickly so here's a movie that spreads out that six microseconds + +58:13.400 --> 58:19.320 +slightly longer to show you a folding of a small protein so this is the end folded structure + +58:19.320 --> 58:24.040 +so you'll see it starts with a linear protein and over six microseconds it goes through all + +58:24.040 --> 58:29.880 +these different confirmations to try and get to the final fold so somehow the linear code limits + +58:29.880 --> 58:33.000 +the number of folds it can take but it tries a number of different ones and if it gets them + +58:33.000 --> 58:39.720 +wrong the protein has to be degraded very quickly or it will cause problems so imagine all the + +58:39.720 --> 58:43.880 +selection that went into this because the protein structure actually determines its rate of folding + +58:43.880 --> 58:50.040 +as well as the final structure in fact the end terminal amino acid determines how fast a protein + +58:50.040 --> 58:56.920 +is degraded this is now called the the end rule pathway for protein degradation for example if + +58:57.000 --> 59:01.640 +you had a amino acid a lysine and arginine or tryptophane as an interminal on a protein beta + +59:01.640 --> 59:07.400 +galactosidase it results in a protein with a half-life of 120 seconds in E. coli or 180 seconds in + +59:07.400 --> 59:11.080 +eukaryotic cell yeast whereas you have three different amino acids a serine of valine or + +59:11.080 --> 59:16.760 +methionine you get a half-life of over 10 hours in bacteria over 30 hours in yeast but these are + +59:16.760 --> 59:22.360 +still relatively short times a bacteria will sell in an hour will have to remake half of all its + +59:22.360 --> 59:29.720 +proteins we make things at almost similar rate but because of this instability and the random + +59:29.720 --> 59:34.920 +folding and misfolding of proteins protein aggregation is a key problem so we have to constantly synthesize + +59:34.920 --> 59:40.360 +new proteins and if they fold wrong you have to get rid of them or they clog up the cell this I + +59:40.360 --> 59:45.640 +think it's absolutely extraordinary that he's spending so much time talking about protein + +59:45.720 --> 59:47.720 +misfolding I can't even believe it + +59:50.600 --> 59:56.360 +he is emphasizing this for like four minutes already that the ribosome isn't very good + +59:56.360 --> 01:00:02.280 +even though it's the most beautiful thing and it is it is the secret to life it's not very + +01:00:02.280 --> 01:00:08.680 +good machine it makes errors all the time and this protein misfolding has to be is a source of + +01:00:08.680 --> 01:00:16.280 +danger like what is he talking about here how can we replace our gut epithelial cells every + +01:00:16.280 --> 01:00:25.080 +three to five days if protein misfolding in RNA and ribosomes is so shitty how the hell would that work + +01:00:28.920 --> 01:00:33.320 +I'm sorry but I feel like what I see here is exactly what I thought I would see here + +01:00:34.040 --> 01:00:39.800 +I'm going to find as we look back on these scientists talking about the human genome project talking + +01:00:39.800 --> 01:00:46.600 +about sequencing talking about the idea of DNA to RNA to protein are going we're going to find + +01:00:46.600 --> 01:00:51.960 +out that we missed it but they were emphasizing protein misfolding for a lot longer than we thought + +01:00:53.560 --> 01:00:57.960 +and not because they have a plethora of evidence about how devastating this is or how important it + +01:00:57.960 --> 01:01:04.920 +is to understand but because they knew it was going to increase in the future and they needed to be + +01:01:04.920 --> 01:01:13.720 +sure that we had a naturally occurring mechanism cartoon in our head already when it started to + +01:01:13.720 --> 01:01:22.200 +become more and more apparent more and more part of our daily lives Alzheimer's cannot be a toxin + +01:01:22.840 --> 01:01:28.840 +or will be angry it cannot be poisoning or will be angry it cannot be something in the water + +01:01:28.840 --> 01:01:34.920 +or will be angry it cannot be electromagnetic radiation or cell phone towers or anything or + +01:01:34.920 --> 01:01:40.120 +we will be angry it has to be a natural thing that just happens to some old people when they get + +01:01:40.120 --> 01:01:50.280 +unlucky or they use too many aluminum pans this guy is laying down a narrative of protein + +01:01:50.280 --> 01:02:00.840 +misfolding that is absolutely ridiculous because they have no absolutely none zero zip evidence + +01:02:00.840 --> 01:02:06.040 +for how it is that if the ribosome is making so many mistakes how in the world do we compensate + +01:02:06.040 --> 01:02:11.400 +for all that wasted energy all that was wasted raw materials that get assembled into something + +01:02:11.400 --> 01:02:17.000 +that just needs to be broken down again how in the world can we take this seriously + +01:02:21.160 --> 01:02:28.600 +when our endothelial cells are replaced weekly our epithelial cells in our gut are replaced + +01:02:28.600 --> 01:02:35.000 +weekly how can we possibly take this guy seriously how much wasted energy is he actually describing + +01:02:35.000 --> 01:02:43.320 +as part of the perfect symphony of life i hear mythology being laid here folks same way as if + +01:02:43.320 --> 01:02:48.920 +you stop taking out the trash in a city and this is definitely not doubling everything comes to a + +01:02:48.920 --> 01:02:53.640 +halt so cells work the same way you have to degrade the proteins you have to pump the trash + +01:02:53.640 --> 01:02:59.160 +out of the cell it can be toxic very quickly there's a number of diseases known to most of you + +01:02:59.960 --> 01:03:03.880 +that are misfolding or aggregation diseases and Alzheimer's disease and macao disease are + +01:03:03.880 --> 01:03:10.280 +examples of accumulation of toxic protein aggregates but recently a new drug came out to deal with + +01:03:10.280 --> 01:03:14.120 +cystic fibrosis and it's quite interesting people thought the mutations in cystic fibrosis + +01:03:14.120 --> 01:03:19.560 +just yielded in a less functioning chloride ion channel that affected the cell's ability + +01:03:19.560 --> 01:03:24.440 +to survive properly it turns out the single mutation that's the major mutation cystic fibrosis + +01:03:24.440 --> 01:03:29.240 +actually blocks the protein from disassociating with its chaperonin and never gets to the cell + +01:03:29.240 --> 01:03:33.480 +surface so simple changes a single letter change in the genetic code in this case doesn't allow + +01:03:33.480 --> 01:03:40.200 +it to fold properly and get associated so lots of diseases are turning out to be problems from + +01:03:40.280 --> 01:03:47.240 +protein folding but it's weird right so it doesn't fold properly but the RNA gets translated by the + +01:03:47.240 --> 01:03:52.120 +ribosome and the system doesn't recognize it as being folded incorrectly so alchemy doesn't get + +01:03:52.120 --> 01:04:00.760 +degraded so there's mechanisms that he talks about then not talk about then ignores then says + +01:04:00.760 --> 01:04:08.200 +are there then says aren't there the incongruencies come every other sentence because he's describing + +01:04:08.200 --> 01:04:13.800 +something with certainty of which he has no actual certainty about and he knows it + +01:04:15.480 --> 01:04:20.280 +and these incongruencies are there because the story of what they understand is incongruent + +01:04:20.280 --> 01:04:26.200 +they don't understand it they're still shooting in the dark and pretending they got this all under + +01:04:26.200 --> 01:04:30.760 +control so you and I don't ask any questions and so that the government keeps funding them + +01:04:31.240 --> 01:04:41.560 +these are the exact charlatans that peter teal was warning us about where the hyper specialization + +01:04:41.560 --> 01:04:49.800 +allows people to lie and to exaggerate the politicization of science allows people encourages people to + +01:04:49.800 --> 01:04:55.000 +lie and exaggerate that's what you see here a man who's done it his whole career + +01:04:55.800 --> 01:05:06.040 +because he's been encouraged to do it by people like David Baltimore and Stanley Plotkin + +01:05:07.800 --> 01:05:18.280 +and all the other people like Robert Gallo and Murray Gardner and everybody else who has + +01:05:18.280 --> 01:05:24.040 +a vested interest in no one actually understanding the biology at the molecular level + +01:05:25.160 --> 01:05:29.080 +so that the biosecurity state can have an imperative to control us forever + +01:05:30.600 --> 01:05:35.320 +and more importantly so that they can invert the sovereignty that we feel over our own bodies + +01:05:35.320 --> 01:05:40.680 +and that of our children so that guys like Craig Venter can finally have the genetic data that + +01:05:40.680 --> 01:05:47.720 +they have already told us many decades ago they need that Mark Lander told us already a decade ago + +01:05:47.800 --> 01:05:53.480 +that we would need without all the genomes we need all of them we're not going to have a chance + +01:05:53.480 --> 01:06:00.680 +of figuring this out that's what the human genome project's final answer was that they got to the + +01:06:00.680 --> 01:06:05.560 +meeting they went to the the secret room in the Department of Energy and they said well we've + +01:06:05.560 --> 01:06:13.480 +got the restriction enzyme maps but they are so a specific that we can barely even see patterns + +01:06:13.480 --> 01:06:19.640 +between people that are closely related so I think the only choice for us is to have as many + +01:06:19.640 --> 01:06:26.280 +genomes as possible we need all the sequences we need them all and so China being partnered + +01:06:26.280 --> 01:06:30.360 +with that with that whole movement just said okay well we'll start collecting ours we're + +01:06:30.360 --> 01:06:38.520 +going to do it right now meanwhile we with our inertia in our system still have this thing called + +01:06:38.600 --> 01:06:45.880 +privacy and and all that other stuff and and so we couldn't convert we couldn't just start + +01:06:45.880 --> 01:06:52.040 +collecting all the data from all our kids and so instead what we did was we had people like Craig + +01:06:52.040 --> 01:06:59.640 +Venter and and people like like James Giordano and others brief secret meetings and tell people + +01:06:59.640 --> 01:07:03.400 +that look China is already collecting all this data and they might even be collecting it on us + +01:07:03.400 --> 01:07:07.320 +too and in the meantime we're not doing anything and at some point in time they're going to have + +01:07:07.320 --> 01:07:12.440 +enough data to make very big progress in cracking the pattern integrity that is the genome and how + +01:07:12.440 --> 01:07:19.080 +it's translated into people or animals or viruses or bacteria and so we don't do what they are doing + +01:07:19.080 --> 01:07:27.160 +we will be behind forever and so their plan was well we'll create a pandemic and we'll + +01:07:27.160 --> 01:07:33.800 +invert the sovereignty that people currently have in our system to permission we might have + +01:07:33.800 --> 01:07:38.200 +to do it gradually over a couple executions of it but that's what we'll do and in the end we'll + +01:07:38.200 --> 01:07:42.760 +collect the data that we need just like the Chinese are collecting the data that they need and that's + +01:07:43.320 --> 01:07:50.120 +what people like Craig Venter say at the back rooms of TED Talks and at at food conferences after + +01:07:50.120 --> 01:07:55.400 +the main audience leaves and they have meetings with the directors and the people that coordinate + +01:07:55.400 --> 01:08:01.320 +these things that's what they talk about that's what Peter Thiel and his friends at the top of + +01:08:01.320 --> 01:08:05.640 +these weaponized piles of money are talking about when they talk about us and how they're + +01:08:05.640 --> 01:08:11.240 +going to use us we are data and if we're not going to give up our data and then you can die at 55 + +01:08:14.040 --> 01:08:19.640 +am I pessimistic yeah I kind of am because it's starting to become obvious to me that this has + +01:08:19.640 --> 01:08:26.680 +been going on for a very very long time and I like everybody else was a victim of it since a child + +01:08:27.400 --> 01:08:31.480 +based on this genetic code so I'm trying to leave you with a notion that life is a process of + +01:08:31.480 --> 01:08:37.880 +dynamic renewal we're all shedding about 500 million uskin cells every day that dust that + +01:08:37.880 --> 01:08:43.000 +accumulates in your home that that's you so you shed your entire outer layer skin every two to + +01:08:43.000 --> 01:08:47.960 +four weeks you have five times 10 to the 11 blood cells that die every day if you're not + +01:08:47.960 --> 01:08:53.400 +constantly synthesizing new cells you're in trouble and can you see how easy it would be to get rid + +01:08:53.480 --> 01:08:59.400 +of virally replicating cells that we're replicating a an RNA they weren't supposed to replicate if + +01:08:59.400 --> 01:09:03.880 +that's what happens in a viral infection do you see how easy it would be if there's such high turnover + +01:09:03.880 --> 01:09:11.080 +at these barriers give me a break ladies and gentlemen well and this surprised me the most + +01:09:11.080 --> 01:09:16.280 +I'm looking at this about half of all our cells die during normal organ development so everything + +01:09:16.280 --> 01:09:20.680 +is constantly turning over and changing looking at cancer cells the half-life of human proteins + +01:09:20.760 --> 01:09:25.960 +was between 45 minutes think about all this turnover of proteins and of cells and of tissues but + +01:09:26.600 --> 01:09:31.400 +all this extremely erroneous protein production that he just described five minutes ago + +01:09:32.840 --> 01:09:37.240 +it just doesn't all jive together it doesn't make sense unless he's trying to lay down + +01:09:37.880 --> 01:09:45.480 +a narrative about how protein misfolding can be caused catastrophic disease states unless he's + +01:09:45.480 --> 01:09:52.680 +actually trying to convince people of this extremely rare possibility there's no reason for + +01:09:52.680 --> 01:10:00.680 +him to talk about the extreme infidelity of of RNA translation by ribosomes and pretend that + +01:10:00.680 --> 01:10:06.440 +there that so many errors in folding are made that that you know we get one out of every what four + +01:10:06.440 --> 01:10:11.080 +proteins are good then or what what's his what's the ratio there because the way that he talks about + +01:10:11.080 --> 01:10:17.160 +it it sounds like it's a really crappy thing as he said if you built a car factory out of ribosomes + +01:10:17.160 --> 01:10:21.720 +you'd go out of business because they don't make cars very well what in the world is he talking + +01:10:21.720 --> 01:10:27.240 +about then if we have such high turnover how do we succeed in getting all that height millions of + +01:10:27.240 --> 01:10:33.400 +cells are being produced with perfect proteins apparently and despite that they're also making + +01:10:33.400 --> 01:10:38.280 +on top of that a bunch of shitty proteins that waste all that energy waste all that ribosomal + +01:10:38.760 --> 01:10:46.680 +uh uh sort of translation time and also waste all that degradation machinery think about how many + +01:10:46.680 --> 01:10:52.440 +proteins that we eat digest into amino acids and then reassemble into garbage that we then + +01:10:52.440 --> 01:11:01.320 +have to digest again according to this guy it's spectacular that's in 22 hours if you take the + +01:11:01.320 --> 01:11:07.240 +DNA out of the cells the cells die uh so this is a key part of things that we were looking into + +01:11:07.240 --> 01:11:12.040 +going forward now as you know all life is cellular and the cellular theory is that you can only get + +01:11:12.040 --> 01:11:17.400 +life from pre-existing cells and people attributed all kinds of special parameters to these cells + +01:11:17.400 --> 01:11:23.560 +over time this is what an artist version view of what a cell cytoplasm might be it's actually + +01:11:23.560 --> 01:11:27.800 +quite a crowded place it's relatively viscous it's not this empty bag with a few proteins + +01:11:27.800 --> 01:11:32.840 +floating around in it and it's a very unique environment so you know why isn't he talking + +01:11:32.840 --> 01:11:38.440 +about the aspect that it's in water and at that scale water is a polar solvent with his + +01:11:38.440 --> 01:11:47.880 +definite shape and so with a simple computer model you can you can show that water can arrange + +01:11:47.880 --> 01:11:55.720 +very well in a compact matrix where the positive and negative dipoles of the of the molecules are + +01:11:55.720 --> 01:12:02.040 +lined up in a pattern that's almost a crystal lattice and thinking about how proteins behave + +01:12:02.040 --> 01:12:08.200 +inside of that highly organized water lattice is crucial to understanding how these things + +01:12:08.200 --> 01:12:14.200 +would come together in this three-dimensional space that is the cytoplasm of the cell instead + +01:12:14.200 --> 01:12:19.400 +he's talking about the proteins without the context in which they are found he's talking + +01:12:19.400 --> 01:12:24.040 +about the proteins without talking about how they are actually electrostatic three-dimensional + +01:12:24.040 --> 01:12:31.960 +shapes that at those size scales have great forces of repulsion great forces of hydrophobicity + +01:12:31.960 --> 01:12:45.880 +and what's the opposite of hydrophobicity hydrophilic behavior so we really need to understand how + +01:12:45.880 --> 01:12:53.400 +many of these really almost infinite variables are being edited out of this reductionist model + +01:12:53.400 --> 01:12:58.520 +of the cell your cytoplasm when he says a pretty crowded place with lots of proteins and stuff + +01:12:58.520 --> 01:13:04.360 +that are all floating around in a very highly organized polar solvent of water + +01:13:05.480 --> 01:13:11.320 +which is again what Luke Montagnier was working on as he died when he passed away he was working + +01:13:11.320 --> 01:13:22.120 +on what happens when water is occupied by biomolecules is it possible that biological molecules + +01:13:22.840 --> 01:13:29.240 +have a property where they cause the water around them to organize now i want you to think about this + +01:13:29.240 --> 01:13:34.920 +because this is again one of those things where the real data and the real ideas have been put + +01:13:34.920 --> 01:13:39.720 +out there and just you've been distracted by people who have said that you can freeze water and capture + +01:13:39.720 --> 01:13:46.120 +the emotion in it like in a picture of a wedding ring or some nonsense like that those kinds of + +01:13:46.120 --> 01:13:53.160 +water memory bullshit stories are out there because Luke Montagnier was onto something + +01:13:54.040 --> 01:13:57.640 +onto something that physicists and chemists have known about for a long time + +01:13:58.600 --> 01:14:02.360 +has something to do with the the surface tension of water and what happens + +01:14:02.360 --> 01:14:07.720 +at this where water becomes at a surface but it's how the water molecules are able to organize + +01:14:07.720 --> 01:14:12.840 +relative to one another and change their properties the way that that you can move through them + +01:14:13.720 --> 01:14:19.880 +and Luke Montagnier was onto the idea that if you had a DNA molecule and a DNA molecule could have + +01:14:20.520 --> 01:14:29.400 +the propensity to organize the water molecules around it you can imagine a scenario where a + +01:14:29.400 --> 01:14:37.000 +a DNA molecule could cause a crystal lattice to form around it in the polar solvent of water + +01:14:37.640 --> 01:14:43.800 +and because of the way that water organizes itself that that shadow in the water could be more than + +01:14:47.400 --> 01:14:51.080 +spun it could exist for longer than the molecules present + +01:14:53.000 --> 01:15:02.520 +and curiously in my humble opinion this would have to play a crucial role in any pathogenic + +01:15:03.480 --> 01:15:11.480 +infectiousness of a prion protein because in between each protein is going to be a water layer + +01:15:12.520 --> 01:15:19.480 +or layers and so in order to understand how a prion protein can cause a non-prion protein to + +01:15:19.480 --> 01:15:25.240 +become a prion protein we need to understand how these two electrostatic shapes complex shapes + +01:15:25.240 --> 01:15:30.520 +can come together in the polar solvent of water and then produce essentially the same structure + +01:15:30.520 --> 01:15:40.760 +function that's the model that we have been given that's the model that we're we're we're + +01:15:40.760 --> 01:15:46.440 +going to try to understand over the coming days and weeks and when we do as we approach that + +01:15:47.400 --> 01:15:52.840 +model and we see how people have have tested and tried to verify you're going to find that nobody + +01:15:52.840 --> 01:15:58.040 +nobody did anything that everybody just keeps making the assumptions that this guy's making + +01:15:58.120 --> 01:16:03.480 +which is that ribosomes make a lot of errors and that sometimes those errors are catastrophic I guess + +01:16:05.480 --> 01:16:09.080 +but if you listen to Craig describe and it sure seems like there should be a lot more + +01:16:09.080 --> 01:16:15.720 +misfolded proteins than than we seem to be aware of and we sure waste a lot of energy on it then + +01:16:15.720 --> 01:16:20.440 +the proteins are actually in solid phase now a key tentative chemistry that's developed + +01:16:20.440 --> 01:16:26.840 +is the notion of synthesis as proof this perhaps dates back to 1828 when Francis Waller first + +01:16:26.840 --> 01:16:31.880 +synthesized urea now this is herald is the end of vitalism because the thought that you could + +01:16:31.880 --> 01:16:38.440 +only get organic molecules from living entities and so a great amount of attention was given to this + +01:16:38.440 --> 01:16:43.160 +but now there's tens of thousands of papers published that either have proof by synthesis as + +01:16:43.160 --> 01:16:48.600 +the starting point or key point of the title we decided to take the same approach of dealing + +01:16:48.600 --> 01:16:53.880 +with DNA and dealing with life back in 1995 when we sequenced the first genome we actually did a + +01:16:53.960 --> 01:16:58.840 +second one that year looking for the cell with a smallest genome and we chose microplasmid genitalium + +01:16:58.840 --> 01:17:04.440 +it has 482 protein coding genes and 43 RNA genes we ask simple questions how many of these genes + +01:17:04.440 --> 01:17:08.760 +are essential for life but what's the smallest number of cells needed for a cellular machinery + +01:17:09.320 --> 01:17:15.160 +and ultimately to answer these questions could we design and construct a minimal DNA genome + +01:17:15.160 --> 01:17:18.920 +of the cell as soon as we went down that route we had new questions would chemistry even allow us + +01:17:18.920 --> 01:17:23.160 +to synthesize a bacterial chromosome and if we could would we just have a large piece of DNA + +01:17:23.160 --> 01:17:28.200 +or could we actually boot it up on the cell like a new chemical piece of software we decided to + +01:17:28.200 --> 01:17:34.440 +start where DNA history started with phi X 174 we chose it as a test synthesis i still think that + +01:17:34.440 --> 01:17:38.760 +he is again casting an enchantment here what he uses that you know see if we can boot it up + +01:17:44.280 --> 01:17:46.440 +if you had a machine + +01:17:46.760 --> 01:17:52.760 +that could take a book + +01:17:55.480 --> 01:17:56.920 +and turn it into a movie + +01:17:58.840 --> 01:18:04.760 +just coming up with an analogy off the cuff here if you had a had a machine that could turn a book + +01:18:04.760 --> 01:18:15.560 +into a movie and i told you that the words were turned into images and sometimes those images + +01:18:15.640 --> 01:18:18.040 +come out wrong but most of the time they come out right + +01:18:20.440 --> 01:18:26.280 +and i can make a copy of the book and i can put it into the machine and it will make the same + +01:18:26.280 --> 01:18:33.240 +movie that i copied the book that i copied would make do we understand the machine + +01:18:36.920 --> 01:18:42.280 +because i believe that's the kind of bamboozlement that's happening here we're being made to believe + +01:18:45.720 --> 01:18:53.320 +that we have a machine that can take a book that's the tape right this is a Turing machine + +01:18:53.320 --> 01:18:57.800 +it can take a tape and it can read the tape and it can make a copy of itself + +01:19:02.920 --> 01:19:09.480 +and so if we copy the tape and put the tape through in the machine do we understand how the + +01:19:09.480 --> 01:19:15.000 +machine works then the answer is no no matter how many ways we make a copy of the tape + +01:19:15.000 --> 01:19:20.200 +or a partial copy of the tape or we put the tape in a different machine and it still works + +01:19:20.200 --> 01:19:26.040 +we still don't understand how the machine works and the machine is life it's a big + +01:19:26.040 --> 01:19:31.000 +ship in a bottle variable that we keep calling it nothing because we can't penetrate it + +01:19:33.480 --> 01:19:38.440 +we can ting the bottle at all different sides and stuff like that but we can't explain how that + +01:19:38.440 --> 01:19:41.640 +ship got assembled in there how it was done or who did it and why + +01:19:45.560 --> 01:19:51.880 +and so make no mistake about it he's not describing a fidelity of understanding he's + +01:19:51.880 --> 01:19:58.200 +actually describing a lack of understanding a horrible lack of understanding this because + +01:19:58.200 --> 01:20:03.880 +you can make very few changes on the genetic code of phi x without destroying viral particles + +01:20:03.880 --> 01:20:07.880 +so Clyde Hutchinson who helped sequence phi x ham smith and i developed a series of new techniques + +01:20:08.280 --> 01:20:12.280 +to correct the errors that take place when you synthesize DNA but the machines that make DNA + +01:20:12.280 --> 01:20:15.880 +are not very accurate and the longer the piece of DNA you make the more errors so we had to find + +01:20:15.880 --> 01:20:20.680 +ways to correct errors we corrected the errors and had this 5 000 letter piece of DNA we injected + +01:20:20.680 --> 01:20:25.320 +into e coli and this is the actual photo of what happened the e coli recognized this synthetic + +01:20:25.320 --> 01:20:29.720 +piece of DNA as normal DNA and the proteins being robots to started reading this piece of genetic + +01:20:29.720 --> 01:20:33.560 +code because that's what their program to do they made what the DNA told them to do is to make viral + +01:20:33.560 --> 01:20:37.640 +proteins the virus self-assembled and it showed us gratitude by killing the cells which is how + +01:20:37.640 --> 01:20:42.600 +we defective we get these clear spots on the cell so the virus self-assembled is another one of + +01:20:42.600 --> 01:20:48.680 +those very large mythologies that completely discounts the possibility like i've said before + +01:20:48.680 --> 01:20:55.400 +and i'm going to say it again you can't make a cuckoo clock make toast you cannot hijack the + +01:20:55.400 --> 01:21:03.240 +machinery of a cell and make it do something that it already doesn't do so cells already produce + +01:21:03.240 --> 01:21:14.200 +exosomes which are are lipoprotein coated vesicles that have receptor agonists on the outside or + +01:21:14.200 --> 01:21:18.200 +targeting proteins on the outside and they have a genetic signal on the inside + +01:21:20.440 --> 01:21:25.640 +and i believe that all tissues likely communicate this way signal to the immune system this way + +01:21:25.640 --> 01:21:31.400 +and communicate their state of homeostasis with the rest of the body in this way and if there are + +01:21:31.480 --> 01:21:37.160 +RNAs which are capable of hijacking this machinery they don't hijack this machinery to make + +01:21:37.720 --> 01:21:44.840 +the cells do something they don't already do they make the cells produce exosomes that contain their + +01:21:44.840 --> 01:21:57.080 +sequence and so he is explaining something again laying down a mythology that precludes any other + +01:21:57.080 --> 01:22:03.320 +interpretation of their limited data set a virus is replicating because and making copies + +01:22:03.320 --> 01:22:09.240 +of itself and hijacking the bacterial machinery because we put the DNA in there and then stuff came + +01:22:09.240 --> 01:22:16.600 +out that couldn't be a natural process that's already there all the time that your that your + +01:22:16.600 --> 01:22:23.960 +experimental transfection is harnessing it couldn't possibly be that it has to be evidence of pathogens + +01:22:24.040 --> 01:22:32.120 +and evidence of the fidelity of these RNA pathogens that's that it's it's another illusion it's + +01:22:32.120 --> 01:22:38.040 +crazy how succinctly it's being done so we call this a situation where the software is building + +01:22:38.040 --> 01:22:42.520 +its own hardware all we did was put a piece of DNA software in the cell and we got out a protein + +01:22:42.520 --> 01:22:49.800 +virus with a nucleic acid a core you could call it was not to make viruses you could call it an + +01:22:49.800 --> 01:22:54.360 +exosome but if they called it an exosome then they would let you know that it's a natural process + +01:22:54.360 --> 01:22:59.240 +that it's a process that already exists inside of the cell so it's not surprising + +01:23:01.480 --> 01:23:05.560 +that we can put a transfection in a cell and that a cell will produce + +01:23:06.840 --> 01:23:12.600 +vesicles that contain the contents of that production it's not unusual because it happens + +01:23:12.600 --> 01:23:16.360 +this we wanted to make something substantially larger we wanted to make an entire chromosome + +01:23:16.520 --> 01:23:20.600 +of a living cell but we thought if we could make viral size chromosomes accurately maybe we could + +01:23:20.600 --> 01:23:24.520 +make a hundred or so of those and find a way to put those together so that's what we did we sent + +01:23:24.520 --> 01:23:30.120 +out set out to sequence these pieces that we made these small pieces we sequenced them verify them + +01:23:30.120 --> 01:23:35.000 +and put them together and it looks like a soccer or basketball playoff so we put four pieces together + +01:23:35.000 --> 01:23:39.320 +and we had pieces now that were 24,000 letters long we would draw these up sequence them in + +01:23:39.320 --> 01:23:43.400 +assemble those together to get pieces that were 72,000 we would do this again it was very + +01:23:43.480 --> 01:23:47.960 +what in the hell are you hearing here except for the assembly of recombinant clones + +01:23:49.720 --> 01:23:55.000 +i thought that that Ralph Barrick invented the no-seum technique where you use restriction enzymes + +01:23:55.000 --> 01:24:04.360 +to stitch things together oh no he didn't invent that oh i see craig venter says craig venter says + +01:24:04.360 --> 01:24:10.600 +that we had restriction enzymes back before 1973 and that we were making recombinant DNA clones + +01:24:10.600 --> 01:24:16.680 +back in the 70s isn't that strange because i thought i was told by all these people in the + +01:24:16.680 --> 01:24:22.200 +magic show that it was Ralph Barrick's no-seum technique and the things that he shared with the + +01:24:22.200 --> 01:24:29.000 +with the Chinese that was so scary are you telling me that we've had restriction enzymes and been able + +01:24:29.000 --> 01:24:37.400 +to make and assemble clones just like he recited already since the 70s and 80s wow what does that + +01:24:37.400 --> 01:24:45.080 +mean for Ralph Barrick as a bad guy i guess he's not really a bad guy anymore + +01:24:47.000 --> 01:24:53.400 +can you see this the guy Kevin McCurnan trying to tell me that i didn't have anything to stand + +01:24:53.400 --> 01:24:59.000 +on i didn't know what i was talking about kevin McCurnan knows this history he was working for + +01:24:59.000 --> 01:25:06.600 +the department of energy and mit and the whitehead institute in this very project he knows the + +01:25:06.680 --> 01:25:13.320 +limitations of those restriction enzyme maps he knows that they need countless more human + +01:25:13.320 --> 01:25:17.880 +genomes to have any clue about what actually is working and how these are organized + +01:25:21.160 --> 01:25:25.320 +as kevin McCurnan ever said that it actually all comes down to understanding the ribosome + +01:25:31.400 --> 01:25:35.000 +i'm going to play that back because this is really important pieces we sequence them verify + +01:25:35.480 --> 01:25:40.120 +and put them together and it looks like a soccer or a basketball playoff so we put four pieces + +01:25:40.120 --> 01:25:44.760 +together and we had pieces now that were 24,000 letters long we would draw the 24,000 letters + +01:25:44.760 --> 01:25:53.240 +long the coronavirus genome is 30,000 bases at most you need to freaking hear this you need to + +01:25:53.240 --> 01:26:00.680 +hear it right now this molecular biology was not invented by Ralph Barrick this molecular + +01:26:00.680 --> 01:26:04.760 +biology was not invented by Ralph Barrick no matter how many people insist it was + +01:26:06.040 --> 01:26:12.680 +i have been saying it since bobby asked me to figure it out in 2022 that they were telling a + +01:26:12.680 --> 01:26:18.120 +story about Ralph Barrick inventing this stuff because these are old school techniques that go + +01:26:18.120 --> 01:26:24.440 +back to the very basics of molecular biology back before 1973 + +01:26:24.440 --> 01:26:32.280 +we are being misled ladies and gentlemen we are knee deep in it + +01:26:34.920 --> 01:26:39.480 +leaves up sequence them and assemble those together to get pieces that were 72,000 + +01:26:39.480 --> 01:26:42.120 +we would do this again it's very laborious let's talk about a year and a half to do + +01:26:42.920 --> 01:26:51.560 +and we got pieces 144,000 letters in length this was 144,000 base pairs in length + +01:26:55.400 --> 01:26:59.480 +don't tell me for one second that we couldn't have made this don't tell me for one second + +01:26:59.480 --> 01:27:04.280 +that we couldn't made enough of it to put all over the place so that the pcr's would be positive + +01:27:04.280 --> 01:27:10.440 +and the sequencing reactions would be exactly what we wanted them to be so that all molecular + +01:27:10.440 --> 01:27:18.040 +biology could be distorted into this very obvious planted phylogenetic tree where nobody pays + +01:27:18.040 --> 01:27:23.080 +attention to anything they paid attention to in 2020 like fear and cleavage sites or hiv inserts + +01:27:23.560 --> 01:27:28.520 +or staphylococcan and tangerine toxin B homologies none of those things that were + +01:27:28.520 --> 01:27:33.480 +screamed about in 2020 were tracked at all despite the fact that we can sequence it 15 + +01:27:33.480 --> 01:27:34.920 +million times in four years + +01:27:38.440 --> 01:27:44.600 +and we're supposed to believe that eco-health alliance a guy like like Peter Dasek and a guy + +01:27:44.600 --> 01:27:50.120 +like like Ralph Barrick are responsible for what just happened here instead of the weaponized + +01:27:50.120 --> 01:27:57.320 +piles of money that are currently trying to permanently have a hold on our our habits + +01:27:59.480 --> 01:28:07.880 +and and our our intuition about reality decades of lying ladies and gentlemen decades of lying + +01:28:07.880 --> 01:28:14.440 +about protein misfolding decades of lying about about DNA to RNA to protein decades of lying about + +01:28:14.440 --> 01:28:20.600 +how we understand it decades of lying about how close we are to understanding all these + +01:28:20.600 --> 01:28:27.640 +nano machines including the most beautiful important one key to all life on earth target of antibiotics + +01:28:27.640 --> 01:28:32.920 +the ribosome beyond the largest piece that ever been synthesized to 30,000 letters + +01:28:33.560 --> 01:28:38.840 +and at this time E. coli didn't like these large pieces of synthetic DNA in them so we + +01:28:38.920 --> 01:28:44.520 +switched to yeast I found out last night this is a city that loves beer that comes from this + +01:28:44.520 --> 01:28:50.440 +same brewer's yeast a beer and bread but aside from fermentation this little cell has remarkable + +01:28:50.440 --> 01:28:55.400 +properties of assembling DNA so all we had to do was put the four synthetic quarter molecules into + +01:28:55.400 --> 01:29:00.520 +yeast with a small synthetic yeast centromere and yeast automatically assembled these pieces + +01:29:00.520 --> 01:29:05.480 +together so that gave us the first synthetic bacterial chromosome and this is what we published + +01:29:05.560 --> 01:29:12.280 +in 2008 this was the largest chemical structure of a defined a structure ever assembled we continued + +01:29:12.280 --> 01:29:16.760 +to work on DNA synthesis and a somebody started out a young postdoc Dan Gibson came up with a + +01:29:16.760 --> 01:29:22.680 +substantial breakthrough instead of the hours the days to years he found out that by putting + +01:29:22.680 --> 01:29:27.160 +three enzymes together with all the DNA fragments in one pot at 50 degrees centigrade for a little + +01:29:27.160 --> 01:29:33.080 +while it would automatically assemble these pieces so it went from a year or so down to an hour + +01:29:33.080 --> 01:29:36.520 +and there's a breakthrough for a number of reasons most importantly it allows us now to + +01:29:36.520 --> 01:29:41.240 +automate these processes so having a simple one step method allows us to go from the digital + +01:29:41.240 --> 01:29:46.280 +code in the computer to the analog code of DNA in a robotic fashion see it's weird that he's not + +01:29:46.280 --> 01:29:51.160 +mentioning Ralph Barrick with all these technologies I really thought that he was responsible for like + +01:29:51.160 --> 01:29:56.520 +half of all the molecular biology techniques that were used in the world but it turns out + +01:29:56.520 --> 01:30:01.400 +that what he did was take proven molecular biology techniques that were developed in the + +01:30:01.400 --> 01:30:06.840 +70s 80s and 90s and applied them to coronaviruses that were really only discovered and became + +01:30:06.840 --> 01:30:21.240 +tractable in 2006 and 7 and 8 the pandemic narrative is is falling apart like wet tissue paper + +01:30:22.280 --> 01:30:26.600 +and this is being scaled up substantially we proved this initially by in just one step + +01:30:26.680 --> 01:30:31.560 +synthesizing the mouse mitochondria genome so I had two teams working one on the chemistry + +01:30:31.560 --> 01:30:35.320 +and one on the biology and it turns out the biology ended up being more difficult than the chemistry + +01:30:35.320 --> 01:30:39.880 +so how do you boot up a synthetic chromosome in a cell this took us a substantial time + +01:30:39.880 --> 01:30:43.960 +at the workout and this paper that we published in 2007 I think is one of the most important + +01:30:43.960 --> 01:30:48.840 +for understanding how cells work and what the future of this field brings so this is where we + +01:30:48.840 --> 01:30:53.480 +describe genome transplantation in simply by changing the genetic code the chromosome in one + +01:30:53.480 --> 01:30:59.160 +cell swapping it out for another we converted one species into another because it's so important + +01:30:59.160 --> 01:31:02.600 +to the theme of what we're doing I'm going to walk you through this a little bit and by the way + +01:31:02.600 --> 01:31:06.680 +these are two of the scientists that led this at Carol Luckteague was the one with a breakthrough + +01:31:06.680 --> 01:31:12.200 +John Glass and there was a large team working with them so we initially did this by isolating + +01:31:12.200 --> 01:31:18.600 +the chromosome from a cell called in my coides and chromosomes are enshrouded with proteins + +01:31:18.600 --> 01:31:21.880 +which is why there was confusion for so many years whether proteins are DNA with the genetic + +01:31:22.760 --> 01:31:27.560 +material we simply did what Avery did we treated the DNA with the proteolytic enzymes removing all + +01:31:27.560 --> 01:31:31.800 +the proteins because if we're making a synthetic chromosome we need to know can naked DNA work + +01:31:31.800 --> 01:31:35.320 +on its own or there are going to be some special proteins needed for transplantation + +01:31:35.320 --> 01:31:38.680 +we added a couple of cassettes to it one so we could select for it and another so it turns + +01:31:38.680 --> 01:31:44.520 +cells bright blue if it got activated and we found a way to get these genome into a recipient cell + +01:31:44.520 --> 01:31:49.080 +a cell m cap or colon which is about the same distance apart genetically from my coides as we are + +01:31:49.080 --> 01:31:53.160 +from mice so relatively close on the order 10 percent are more different + +01:31:53.160 --> 01:31:55.240 +but let me show you what happened we have this very sophisticated movie + +01:31:56.360 --> 01:32:02.120 +so we inserted the n-microious chromosome into the recipient cell and just as with the phiaxis + +01:32:02.120 --> 01:32:06.920 +soon as we put this DNA into the cell the protein robots started producing mRNA started producing + +01:32:06.920 --> 01:32:11.240 +proteins some of the early proteins were the restriction enzymes that ham smith discovered + +01:32:11.240 --> 01:32:16.360 +in 1970 they recognized the initial chromosome in the cell as foreign DNA and shoot it up + +01:32:17.240 --> 01:32:22.360 +so now we have the body and all the proteins in one species and the genetic software of another + +01:32:22.360 --> 01:32:27.160 +so what happened in a very short period of time we had these bright blue cells when we + +01:32:27.160 --> 01:32:31.880 +interrogated the cells they had only the transplanted genome but more importantly when we sequenced + +01:32:31.880 --> 01:32:36.440 +the proteins in these cells there wasn't a single protein or other molecule from the original + +01:32:36.440 --> 01:32:42.200 +species every protein in the cell came from the new DNA that we inserted into the cell life + +01:32:42.840 --> 01:32:47.400 +is based on DNA software we're a DNA now did you listen because I didn't hear that the cells divided + +01:32:48.760 --> 01:32:55.720 +what I heard was that he transfected the cells the DNA was translated there were enzymes that + +01:32:55.720 --> 01:33:03.800 +were present that degraded the other genome and by the time it had transfected the whole dish + +01:33:03.800 --> 01:33:10.760 +then they had blue cells that were not expressing their own proteins but the ones that they were + +01:33:10.760 --> 01:33:16.360 +transfected with that's not that I'm sorry but I'll look up the paper later but that's not + +01:33:16.920 --> 01:33:22.760 +artificial life that's not reprogramming a cell that's just transfection or transformation + +01:33:22.760 --> 01:33:32.280 +of a of a bacteria and again I'm arguing that I think Craig Venter like Peter Thiel suggested + +01:33:32.280 --> 01:33:38.040 +has a vested interest in lying and exaggerating about the significance of these these experiments + +01:33:38.040 --> 01:33:43.400 +with the idea of making sure that he stays an expert in his hyper specialized field that he + +01:33:43.400 --> 01:33:48.440 +keeps getting all of the the funding and that nobody questions his actual progress and the + +01:33:48.440 --> 01:33:55.400 +significance of it and nobody will right because these people all need us to believe that it's + +01:33:55.400 --> 01:34:00.920 +just a matter of time you might as well go limp where we are about to to solve all of these problems + +01:34:01.640 --> 01:34:07.080 +a software system you change the DNA software you change the species it's a remarkably simple + +01:34:07.160 --> 01:34:12.520 +concept remarkably complex in its execution now we had a problem that some of you may have + +01:34:12.520 --> 01:34:17.080 +picked up on or have read about we were assembling the bacterial chromosome in a eukaryotic cell + +01:34:17.800 --> 01:34:21.320 +so we're going to take the synthetic genome and do the transplantations we had to find a way to + +01:34:21.320 --> 01:34:25.880 +get the genome out of yeast that transplanted back into a bacteria and we developed a whole new way + +01:34:25.880 --> 01:34:30.280 +just to grow bacterial chromosomes in yeast as eukaryotic chromosomes and it was remarkably + +01:34:30.280 --> 01:34:35.400 +simple in the end all we do is add a very small synthetic centromere from yeast to the bacterial + +01:34:35.400 --> 01:34:38.600 +chromosome and all of a sudden it turns into a eukaryotic chromosome the centromere is when + +01:34:38.600 --> 01:34:42.360 +you look at pictures of chromosomes it's that little piece in the middle of the y for example + +01:34:42.360 --> 01:34:46.680 +so now we can stably grow bacterial chromosomes in yeast so we had the situation where we had + +01:34:46.680 --> 01:34:50.040 +the emicoidy's chromosome in the eukaryotic cell we could try isolating it and doing the + +01:34:50.040 --> 01:34:55.000 +transplantation the trouble is it didn't work and this little problem took us two and a half years + +01:34:55.000 --> 01:34:59.480 +to solve of why it didn't work and it turns out when we initially did the transplantations taking + +01:34:59.480 --> 01:35:04.520 +the chromosome out of the emicoidy cell that DNA had been methylated and that's how cells + +01:35:04.520 --> 01:35:11.640 +protect their own DNA from interloping species so we proved this by isolating the six methylases + +01:35:11.640 --> 01:35:15.720 +and methylating the DNA when we took it out of yeast if we methylated the DNA we could then do + +01:35:15.720 --> 01:35:21.080 +the transplantation we proved this ultimately by in the recipient cell removing the restriction + +01:35:21.080 --> 01:35:25.640 +enzymes and in that case we can just put a naked unmetallated DNA because there's nothing to + +01:35:25.640 --> 01:35:30.120 +destroy the DNA in the cell so we're at this point now where we thought we had solved all the problems + +01:35:30.920 --> 01:35:35.240 +we could create these new bacterial strains from the bacterial genomes cloned in yeast + +01:35:35.240 --> 01:35:38.280 +and we had this nice cycle we could work our way around the circle we could add a center + +01:35:38.280 --> 01:35:42.840 +mirror to the bacterial chromosome and turn it into a eukaryotic chromosome the advantages for + +01:35:42.840 --> 01:35:46.440 +those of you who work with bacteria most bacteria do not have genetic systems which is why most + +01:35:46.440 --> 01:35:50.520 +scientists don't work with them as soon as you put that bacterial genome in yeast we have the + +01:35:50.520 --> 01:35:54.680 +complete repertoire of genetic tools in yeast such as homologous recombination so we can make + +01:35:54.680 --> 01:35:59.160 +rapid changes in the genome isolate the chromosome methylated if necessary and do a transplantation + +01:35:59.160 --> 01:36:04.280 +to create a highly modified cell so at this stage we decided to synthesize the mycoides genome + +01:36:04.280 --> 01:36:07.800 +with all these new synthesis techniques Dan Gibson took this on almost single-handedly + +01:36:08.360 --> 01:36:13.240 +if 1.1 million base pairs we started with pieces that were 1,000 letters long we put 10 of those + +01:36:13.240 --> 01:36:16.680 +together to make pieces that were 10,000 letters long we put 10 of those together to make pieces + +01:36:16.680 --> 01:36:22.040 +now that are 100,000 letters long and we had these 11 100,000 base pair pieces we put them in yeast + +01:36:22.040 --> 01:36:26.600 +yeast assembled the DNA we knew how to transplant it out of yeast we did the transplantation and it + +01:36:26.680 --> 01:36:31.720 +didn't work so those of you are software engineers that software engineers have debugging software + +01:36:31.720 --> 01:36:35.560 +to tell them where the problem is in their code so we had to develop the biological version of + +01:36:35.560 --> 01:36:39.720 +debugging software which was basically substituting natural pieces of DNA for the synthetic ones + +01:36:39.720 --> 01:36:44.280 +until we could find out what was wrong we found out we could have 10 of the 11 synthetic pieces + +01:36:44.280 --> 01:36:49.480 +and the last piece I had to be native DNA to get a living cell now what I do think Robert + +01:36:50.680 --> 01:36:56.040 +Ralph Barrick did contribute is what he's talking about now which is idea of coming up with the + +01:36:56.040 --> 01:37:05.560 +minimum coronavirus genome to get exosomes to be produced and so Ralph Barrick used very + +01:37:05.560 --> 01:37:12.200 +standard restriction enzyme ligation techniques to create synthetic DNA versions of RNA viruses + +01:37:13.320 --> 01:37:18.440 +but more importantly because the RNA virology that they had at that time was very sketchy and + +01:37:18.440 --> 01:37:24.120 +only could find the RNA to pet an RNA polymerase or this protein or that protein but not the whole + +01:37:24.120 --> 01:37:31.560 +genome one of the contributions that Ralph Barrick made was that here are the + +01:37:32.200 --> 01:37:37.800 +minimum number of proteins that you need and so if you find a spike protein in the wild that you + +01:37:37.800 --> 01:37:43.800 +want to test you can add that spike protein to all of these now why is that important ladies and + +01:37:43.800 --> 01:37:50.600 +gentlemen please let's go back to the frontier why is that important because in a viral replicating + +01:37:50.600 --> 01:37:57.560 +cell according to their cartoon by far and away the most abundant RNA present is the spike protein + +01:37:57.560 --> 01:38:04.120 +by several orders of magnitude perhaps hundreds of thousands of more spike protein sub genomic + +01:38:04.120 --> 01:38:13.320 +RNAs than any other transcript including the end protein and definitely including the probably + +01:38:13.400 --> 01:38:23.160 +protein of 1a1 or rf1a in other words if you were to do a PCR test in a bat and you were looking for + +01:38:23.160 --> 01:38:29.160 +novel coronaviruses their strategy was to look for the most conserved gene which was the RNA + +01:38:29.160 --> 01:38:35.160 +dependent RNA polymerase and the most abundant one which was the spike protein and they look + +01:38:35.160 --> 01:38:40.360 +for a conserved region of the spike protein because as you know spike proteins are highly + +01:38:40.360 --> 01:38:45.480 +homologous because they have this conformational change that allows membranes to come together + +01:38:45.480 --> 01:38:53.160 +we use a very similar protein in our in our developing embryo yada yada yada so our immune + +01:38:53.160 --> 01:39:00.920 +system looks for that commonality that conserved hydrophobic damage associated molecular pattern + +01:39:03.160 --> 01:39:08.280 +and so my point is is that they looked for those two signals if they found them then Ralph Barrick + +01:39:08.440 --> 01:39:13.720 +had a whole set of genes that you could just put in with that spike protein and now you could make + +01:39:13.720 --> 01:39:23.640 +a synthetic clone of a purported novel coronavirus if you just found the sub genomic RNA of the + +01:39:23.640 --> 01:39:30.120 +spike protein that's how it was done from 2005 until very very recently ladies and gentlemen + +01:39:31.400 --> 01:39:36.680 +exactly as he's describing it where you come up with a minimum set of genes + +01:39:36.760 --> 01:39:42.280 +and by substituting natural ones in until it grows or until it works you can eliminate + +01:39:43.480 --> 01:39:47.800 +the genes you don't need find the genes that you do need and now you have a minimum set + +01:39:49.640 --> 01:39:54.680 +and that's what Ralph Barrick did he didn't invent no serum technologies what he did + +01:39:54.680 --> 01:40:01.880 +was identified the minimum set of genes that would be necessary for an RNA to cause reproduction + +01:40:01.880 --> 01:40:08.680 +of itself and packaging into exosomes not something that the cell doesn't normally do not hijacking + +01:40:08.680 --> 01:40:14.680 +the cell and reprogramming it to make viruses for the first time in its existence but to hijack + +01:40:14.680 --> 01:40:21.080 +the already existing exosomal production machinery to reproduce package and send out the RNA + +01:40:22.600 --> 01:40:28.680 +and I think that Ralph Barrick and these coronavirus people found the minimum set of genes proteins + +01:40:28.680 --> 01:40:33.880 +it's necessary to get that phenomenon to happen and they are telling you that that's a pathogen + +01:40:33.880 --> 01:40:40.200 +that does it when in reality they are hijacking the synthetic machinery of a cell to try and + +01:40:40.200 --> 01:40:48.120 +understand how the hell it works absolutely no different than the illusion created by this + +01:40:48.120 --> 01:40:53.640 +guy claiming to try to create synthetic life I really think we're on to this + +01:40:53.720 --> 01:40:58.040 +re-sequenced it and found one letter wrong and an essential gene made the difference between life + +01:40:58.040 --> 01:41:02.200 +and no life so it was in the DNA gene which is important for starting the whole process of binding + +01:41:02.200 --> 01:41:08.360 +to DNA we corrected that error so one out of 1.1 million we corrected that error and we got the first + +01:41:08.360 --> 01:41:14.520 +actual synthetic cell from the genome transplants one of the ways that we knew this was a synthetic + +01:41:14.520 --> 01:41:20.360 +cell we watermarked the DNA so we could always tell our synthetic species from any naturally occurring + +01:41:21.240 --> 01:41:26.840 +one this was reported in early 2010 and science published it shortly thereafter + +01:41:27.480 --> 01:41:31.880 +the watermarks we watermarked the first genome that we did just using the single letter amino acid + +01:41:31.880 --> 01:41:37.240 +code and we were accused of not having much of an imagination so for this new genome we went a + +01:41:37.240 --> 01:41:42.120 +little bit further and I added three quotations from the literature but first we developed a + +01:41:42.120 --> 01:41:45.560 +whole new code where we could write the english language complete with numbers and punctuation + +01:41:45.560 --> 01:41:49.560 +in DNA code and it's quite interesting we set the paper to science for review and one of the + +01:41:49.560 --> 01:41:53.080 +reviewers sent back the review written in DNA code much to the frustration of the + +01:41:53.080 --> 01:41:57.720 +science editor who could not do separate but the reviewers DNA code was based on the ASCII code + +01:41:58.680 --> 01:42:03.080 +and with biology that creates a problem because you can get long stretches without a stock code + +01:42:03.080 --> 01:42:06.920 +on so we developed this new code that puts in very frequent stock code ons because the last thing + +01:42:06.920 --> 01:42:10.440 +you want to do is put in a quote from James Joyce and have it turn into a new toxin that kills + +01:42:10.440 --> 01:42:17.640 +the cell or kills you you didn't know poetry could do that I guess so we built in the names of the + +01:42:17.720 --> 01:42:22.520 +46 scientists that contributed to this and also there was a message with a URL so being the first + +01:42:22.520 --> 01:42:25.880 +species to have a computer as a parent we thought it was appropriate it should have its own web + +01:42:25.880 --> 01:42:30.120 +address built into the genome and as people solve this code they would send an email to the web + +01:42:30.120 --> 01:42:36.840 +address written in the genome and once numerous people solved it we made this available and let + +01:42:36.840 --> 01:42:40.680 +me just I know what this is hard to read the three quotes are the first one and probably the most + +01:42:40.680 --> 01:42:45.400 +important one to Ireland is James Joyce the live to air to fall the triumph to recreate life out + +01:42:45.480 --> 01:42:50.280 +of life somehow that seemed highly appropriate the second is from Oppenheimer's biography American + +01:42:50.280 --> 01:42:55.080 +Prometheus see things not as they are but as they might be in the third from Richard Fineman what + +01:42:55.080 --> 01:43:01.640 +I cannot build I cannot understand everybody thought it was very cool until a few months after this + +01:43:01.640 --> 01:43:08.200 +appeared we got a letter from James Joyce estate attorney saying did you seek permission to use + +01:43:08.200 --> 01:43:13.960 +this quotation and in the US at least there's fair use laws allow you to quote up to a paragraph + +01:43:13.960 --> 01:43:18.440 +without seeking permission so we sort of dismissed that one then James Joyce was dead we didn't know + +01:43:18.440 --> 01:43:23.080 +how to ask him anyway and then we started getting an email trail from a Caltech scientist saying + +01:43:23.080 --> 01:43:26.840 +we'd misquoted Richard Fineman but if you look on the internet this is the quotation that you find + +01:43:26.840 --> 01:43:31.080 +everywhere and we argued back this is what we found and this was what was in his biography + +01:43:31.080 --> 01:43:34.840 +instead of proof his point he sent a picture of Fineman's blackboard with the original quotation + +01:43:35.720 --> 01:43:42.520 +and what it was what I cannot create I do not understand what I cannot create I do not understand + +01:43:42.520 --> 01:43:51.720 +and so rather than that being a a sort of a cardinal teaching of sacred biology which it should be + +01:43:52.920 --> 01:43:55.880 +you can't create it so you don't understand it we can try + +01:43:57.880 --> 01:44:05.640 +but what he's arguing of course is is that because he claims to have created it he understands it + +01:44:06.200 --> 01:44:12.360 +that's what he's arguing here that's the arrogance that he's putting out here in a very + +01:44:12.360 --> 01:44:19.720 +indirect way it is ugly it is an ugly arrogance make sure you see it + +01:44:22.280 --> 01:44:26.200 +I think it's actually a much better quotation and we've gone back to correct the DNA code + +01:44:26.200 --> 01:44:33.080 +so that Fineman can rest much more peacefully so we can actually go much further now there's + +01:44:33.080 --> 01:44:38.840 +an exciting paper that includes out of Stanford that includes John Glass from my institute using + +01:44:38.840 --> 01:44:43.240 +our work on the micro plasma cell to do the first complete mathematical modeling of a cell + +01:44:43.960 --> 01:44:46.920 +now I was going to show you the movie of their mathematical model but it has so many different + +01:44:46.920 --> 01:44:50.280 +components it would take about 20 minutes to show but this is coming out in cell I think + +01:44:50.280 --> 01:44:54.520 +next week it is going to be an exciting change so we can go from the digital code to the genetic + +01:44:54.520 --> 01:45:00.360 +code and now modeling the entire function of the cell in a computer going to complete a digital + +01:45:00.360 --> 01:45:05.160 +circle we're going further now we're using computer software to design new DNA software + +01:45:05.160 --> 01:45:12.120 +to create a new cells and this is an important part of what I'll be talking about on a Saturday + +01:45:12.120 --> 01:45:17.480 +evening now in conclusion I hope it's starting to become clear if it wasn't already that all + +01:45:17.480 --> 01:45:24.280 +living cells that we know of on this planet are DNA driven DNA software driven biological machines + +01:45:24.280 --> 01:45:29.480 +comprised of hundreds to thousands of proteins and these are protein robots coded for by the DNA + +01:45:30.440 --> 01:45:35.400 +these protein robots carry out these very precise functions and developed by billions of years of + +01:45:35.400 --> 01:45:39.240 +evolution you make a change in the DNA code it makes a change in the protein code that can + +01:45:39.240 --> 01:45:42.280 +affect whether the protein is functional or not it can affect whether the cell lives or not + +01:45:42.280 --> 01:45:48.520 +billions of years of evolution that he's not at all concerned about screwing up billions of + +01:45:48.520 --> 01:45:54.040 +years of evolution that he's not at all worried about his lack of understanding of to go ahead + +01:45:54.600 --> 01:45:59.320 +and start thinking that everything is a nail it can affect the rate that the protein folds how + +01:45:59.320 --> 01:46:04.200 +fast it is degraded this is all programming built into life that controls the three-dimensional + +01:46:04.200 --> 01:46:11.160 +structure so this is dynamic regulation and explains most of what Darwin described as Darwinian + +01:46:11.160 --> 01:46:16.440 +evolution by making a copy of the DNA software the cell can self-replicate making another copy + +01:46:16.440 --> 01:46:21.800 +of itself as Schrodinger described all these processes require energy and from all the genome + +01:46:21.800 --> 01:46:26.920 +two sequences a range of mechanisms that cell cells can provide energy as you know if we eat + +01:46:26.920 --> 01:46:31.400 +sugar that's one way to do it and with simple metabolism it's been well worked out pathways for + +01:46:31.400 --> 01:46:35.960 +decades how we extract the energy contained in the sugar molecule converting it into cellular + +01:46:35.960 --> 01:46:40.040 +energy that can drive all these different processes in 1996 we sequenced the genome of + +01:46:40.040 --> 01:46:44.120 +methanococcus genacia the first archaea and the first autotrof that means it makes everything + +01:46:44.120 --> 01:46:49.080 +from life from inorganic substances and it makes its energy by converting co2 into nothing it also + +01:46:49.080 --> 01:46:54.280 +uses the carbon and co2 to make all its proteins in lipids all these processes are coded for in + +01:46:54.360 --> 01:47:00.280 +the DNA we've shown now using synthetic genomes when you put new DNA software into the cell the + +01:47:00.280 --> 01:47:04.600 +protein robots read it immediately start producing the proteins coded for changing the cellular + +01:47:04.600 --> 01:47:09.560 +phenotype and when you change the DNA software you change the species this is all consistent with + +01:47:09.560 --> 01:47:14.360 +Schrodinger's code script and organisms astonishing gift of concentrating a stream of order on itself + +01:47:15.320 --> 01:47:19.000 +Schrodinger citing the second law of thermodynamics the entropy principle the natural + +01:47:19.000 --> 01:47:22.920 +tendency of things to go over into disorder describe the notion of order based on order + +01:47:22.920 --> 01:47:26.200 +we get the order from the genetic code we get the order from its interpretation into + +01:47:27.960 --> 01:47:32.600 +we can digitize life and regenerate life from the digital world and just as the ribosome can + +01:47:32.600 --> 01:47:38.120 +convert the analog message and mRNA into a protein robot it's becoming standard now in the world + +01:47:38.120 --> 01:47:45.000 +of science to convert digital code into proteins viruses and now cells scientists send digital + +01:47:45.000 --> 01:47:49.160 +code to each other instead of sending genes or proteins and there's several companies around + +01:47:49.160 --> 01:47:54.440 +the world that make their living by synthesizing genes for scientific labs it's faster and cheaper + +01:47:54.440 --> 01:48:00.440 +to synthesize a gene than is the clonet or even get it by federal express an example of this + +01:48:00.440 --> 01:48:06.040 +digital biological conversion is we've been working on new ways to make vaccines using synthetic DNA + +01:48:06.040 --> 01:48:10.040 +and we progress this process very efficiently and now working with barda in the u.s. government + +01:48:10.040 --> 01:48:15.000 +and novartis barda sends us an email containing a test pandemic flu sequence and we have less + +01:48:15.000 --> 01:48:20.760 +than 24 hours to convert that digital code into a virus we now have that process under 12 hours + +01:48:20.760 --> 01:48:25.960 +we get that to novartis and they're ready to scale up production for vaccines now we're in the process + +01:48:25.960 --> 01:48:32.520 +of building a much smaller simpler what i call digital conversion device so my digital biological + +01:48:32.520 --> 01:48:37.000 +converter is going to work somewhat like the telephone where it takes digital waves and converts + +01:48:37.000 --> 01:48:41.560 +it into sound waves so you can hear it only will have a small box that you could attach to a computer + +01:48:42.200 --> 01:48:48.600 +to in fact receive a digital wave and convert it into a protein perhaps a vaccine or even a cell + +01:48:49.240 --> 01:48:55.800 +so think about in a future flu pandemic we could this sounds a lot like that that thing that David + +01:48:55.800 --> 01:49:00.840 +Hone talked about where you got a arm band on one side that reads the pathogen and arm band on the + +01:49:00.840 --> 01:49:06.200 +other side that makes the vaccine and injects it in you is he not talking about exactly the same + +01:49:06.200 --> 01:49:10.520 +thing am i a am i a crazy person here barda and novartis + +01:49:12.200 --> 01:49:16.200 +we distribute a new vaccine in seconds around the world because this is biology now moving + +01:49:16.200 --> 01:49:22.520 +at the speed of light perhaps even through each individual home oh no this wasn't a plan there's + +01:49:22.520 --> 01:49:27.480 +no way that this was a plan how could you possibly believe it's a plan if it was a plan they would + +01:49:27.480 --> 01:49:34.040 +have been talking about what they were going to do for years already uh all life is derived + +01:49:34.040 --> 01:49:39.080 +currently from other cellular life but i think it's just a short matter of time before someone + +01:49:39.080 --> 01:49:44.120 +discovers the right chemical cocktail of enzymes ribosomes lipids together with the synthetic genome + +01:49:44.120 --> 01:49:49.080 +to just to create a simple boot up system to get cells without a prior cellular history + +01:49:49.720 --> 01:49:54.200 +wow that's amazing i'm sure it's you know only a matter of time before we are curing childhood + +01:49:54.200 --> 01:49:59.400 +diseases with retroviruses in every childhood her every uh children's hospital in america i'm + +01:49:59.400 --> 01:50:04.200 +sure it's not very long before the futurist dream of being able to look at your kid and + +01:50:04.200 --> 01:50:08.920 +pick all the phenotypes you want and dial up the intelligence and musical creativity + +01:50:08.920 --> 01:50:13.240 +will be as simple as calling craig ventors uh company and saying sequence me + +01:50:15.640 --> 01:50:18.520 +so let's look at the tremendous progress in this last seven years and shorteners + +01:50:18.520 --> 01:50:23.080 +lecture on this campus and try to imagine things 70 years from now uh jim watson still with us + +01:50:23.080 --> 01:50:28.600 +whether he'll be with us 70 years from now is an open question try to imagine 70 years from now + +01:50:28.600 --> 01:50:36.360 +is exactly the bamboozlement and mythology they want you to do by imagining a future that he just + +01:50:36.360 --> 01:50:42.440 +brainwashed you into believing in or imagining you are accepting the model of reality that is in + +01:50:42.440 --> 01:50:49.880 +that imaginary model in that imaginary image that model is what he just got through describing + +01:50:49.880 --> 01:50:58.280 +which is that we are the summation of chemistry and physics there's nothing else + +01:50:59.160 --> 01:51:02.760 +protein machines mindlessly do what they're told + +01:51:04.520 --> 01:51:08.200 +and we are nothing more than a Turing machine that reads a tape + +01:51:10.360 --> 01:51:16.280 +that's what craig vetner that's what all the people at the human genome project that's what + +01:51:16.280 --> 01:51:21.800 +all the people that want to enslave your grandchildren want you to believe about your biology + +01:51:22.440 --> 01:51:28.120 +about your children's biology and about the flawed nature of every child that's born + +01:51:29.720 --> 01:51:35.160 +when in reality it's exact inversion of the truth children are born perfect + +01:51:36.680 --> 01:51:42.840 +and even if they're born imperfect it is a perfect imperfect that cannot be augmented + +01:51:43.400 --> 01:51:50.360 +through any rudimentary understanding that we think we have of this pattern integrity + +01:51:51.560 --> 01:51:53.880 +it can't it simply cannot + +01:51:55.480 --> 01:52:00.120 +but certainly some of you will be here in 70 years so in the year 2082 what will be happening + +01:52:00.760 --> 01:52:06.440 +well we just saw my friend Elon Musk launch a private space flight to go up to the space station + +01:52:06.440 --> 01:52:10.520 +certainly within 70 years we will have colonized the moon and Mars there's several working on trying + +01:52:10.520 --> 01:52:15.640 +to do that so just like new life forms for food in 70 years we're going to be on Mars you see + +01:52:15.640 --> 01:52:21.000 +where we're going here and I mean Robert Malone thought within 10 years we would be curing childhood + +01:52:21.000 --> 01:52:26.360 +diseases with retroviruses and we're at least 30 years off on that if not 40 + +01:52:28.360 --> 01:52:34.200 +WTF ladies and gentlemen how can we take these people seriously when they've been doing exactly + +01:52:34.200 --> 01:52:40.040 +what Peter Teal said they've been doing which is lying and exaggerating because of the hyperspecialization + +01:52:40.040 --> 01:52:47.080 +completely allows it and because we're producing so much knowledge i.e. noise + +01:52:48.440 --> 01:52:54.440 +with this endless reiterative falsification of hypotheses driven by preparean thinking + +01:52:57.080 --> 01:53:02.680 +and every one of these people knows it Peter Teal knows it Elon Musk knows it + +01:53:03.320 --> 01:53:07.480 +the Weinstein's probably know it because they've probably had it explained to them by their father + +01:53:07.560 --> 01:53:08.280 +by these guys + +01:53:12.280 --> 01:53:16.120 +it's an elaborate hoax ladies and gentlemen the quicker that we can teach it to our kids the quicker + +01:53:16.120 --> 01:53:22.840 +that we break free energy production new medicines perhaps a vaccine could be sent from earth to + +01:53:22.840 --> 01:53:28.600 +Mars at the speed of light taking somewhere between 4.3 and 21 minutes to get their depending on the + +01:53:28.600 --> 01:53:33.560 +distance between earth and Mars the digital and biological worlds are now becoming interchangeable + +01:53:34.280 --> 01:53:39.160 +perhaps more importantly and i'll speak to this again on on Saturday evening synthetic life + +01:53:39.160 --> 01:53:43.000 +is going to be the tool that allows us to understand the diversity of life on this planet + +01:53:43.000 --> 01:53:47.160 +and hopefully enable new industries to produce food fuel clean water and medicine + +01:53:48.040 --> 01:53:53.000 +as we add a billion people to the planet over the next 12 years and every 12 years of following + +01:53:53.000 --> 01:53:57.400 +that as we'll hear in a few minutes and you heard in the introduction Schrodinger's life + +01:53:57.400 --> 01:54:02.520 +helps to stimulate the Jim Watson and Francis Crick over 60 years ago to help kick off this new + +01:54:02.520 --> 01:54:08.520 +era of the DNA world with their discovery of the double helix one can only hope that this new + +01:54:08.520 --> 01:54:13.400 +frontier of synthetic life will have a similar impact on the future thank you very much + +01:54:21.720 --> 01:54:27.640 +yeah go back and sit down there sir ladies and gentlemen this has been giga ohm biological + +01:54:27.640 --> 01:54:33.240 +it is a high resistance low noise information brief brought to you by a biologist make no + +01:54:33.240 --> 01:54:39.160 +mistake about it ladies and gentlemen why is it doing that dang it i clicked on that slide like + +01:54:39.160 --> 01:54:48.440 +three times now click click current slide there we go so we are in a trap right now ladies and + +01:54:48.440 --> 01:54:54.200 +gentlemen we are in a trap and we are being trapped in this new world order where all of these things + +01:54:54.200 --> 01:54:59.560 +that peter vetner said are about to come true all of this virology is real all this pandemic + +01:54:59.560 --> 01:55:05.480 +potential is real and if we allow this mythology to be passed on to our children our children will + +01:55:05.480 --> 01:55:13.800 +be enslaved by these machines by these softwares and by these apps not by the ai to come but by the + +01:55:13.800 --> 01:55:22.120 +algorithms currently deployed and by the liars currently lying ladies and gentlemen please stop + +01:55:22.200 --> 01:55:26.920 +all transfections in humans because they are trying to eliminate the control group by any means + +01:55:26.920 --> 01:55:34.520 +necessary intramuscular injection of any combination of substances with the intent of augmenting the + +01:55:34.520 --> 01:55:40.440 +immune system is dumb transfection is criminally negligent and viruses are not pattern integrity + +01:55:40.440 --> 01:55:44.600 +so thanks very much for joining me i will see you again tomorrow i have an interview with + +01:55:44.600 --> 01:55:50.760 +thomas binder and we are trying to decide whether we want to do 12 o'clock or i rather one o'clock + +01:55:50.840 --> 01:55:54.760 +and have two hours and i think that's what we're going to do right now it's scheduled for two + +01:55:55.320 --> 01:56:00.520 +but don't be surprised if it starts at one or if it's scheduled for one don't be surprised if it + +01:56:00.520 --> 01:56:05.720 +starts at twelve because i'm going to try and get two hours with thomas instead of one so + +01:56:05.720 --> 01:56:09.880 +he's offered an earlier hour i'm going to take it and then i'll change the schedule when i have + +01:56:09.880 --> 01:56:14.280 +confirmation i'll see you tomorrow for thomas binder it's going to be a great discussion one of my + +01:56:14.280 --> 01:56:20.680 +favorite people on the interwebs with regard to the pandemic and speaking truth to this power + +01:56:21.720 --> 01:56:26.680 +i'm really excited about that you can find me at giggleonbiological.com don't underestimate how + +01:56:26.680 --> 01:56:32.200 +much we are sacrificing for this it might look like this is a very rich production but it's a + +01:56:32.200 --> 01:56:38.840 +couple of free cameras got a couple of craigslist hardware devices and an old mac that's what we're + +01:56:38.840 --> 01:56:45.480 +doing this with and my family is absolutely positively living off of this stream so + +01:56:45.480 --> 01:56:49.160 +if you can see that i've got about a hundred and ten subscribers that's about a thousand + +01:56:49.160 --> 01:56:54.840 +dollars a month we have two thousand four hundred dollars a month in rent because we rent from + +01:56:54.840 --> 01:57:01.800 +a weaponized pile of money that's starting to take over the residential property in in + +01:57:01.880 --> 01:57:07.560 +pittsburgh so i'm i'm asking for help i'm begging for help i need everybody's help and if you are + +01:57:07.560 --> 01:57:14.520 +already helping then share if you are already helping then share and if you can't help please + +01:57:14.520 --> 01:57:19.800 +share that's about all i can say because that's what we need we need millions of viewers thousand + +01:57:19.800 --> 01:57:25.320 +subscribers and we can actually win i'm convinced of it thank you very much for joining me and i'll + +01:57:25.320 --> 01:57:32.920 +see you again soon tomorrow for sure intellectual bright web we'll be back tomorrow for sure thanks + +01:57:55.960 --> 01:57:57.960 +you + +01:58:13.320 --> 01:58:19.960 +i would be very up for meeting anyone in northern corner of western pennsylvania for a picnic + +01:58:19.960 --> 01:58:25.000 +on the eighth of april if you want to organize that with me you got to email me directly i'm not + +01:58:25.080 --> 01:58:30.200 +gonna tell everybody in the world where my little family's gonna go but i would be happy to have + +01:58:30.200 --> 01:58:36.520 +anybody who wants to join me it is the eighth it is i think two monday's from now um we won't + +01:58:36.520 --> 01:58:42.040 +have a chance to see this again without a big travel expense so hopefully we're gonna get clear + +01:58:42.040 --> 01:58:47.640 +weather um my boss at the university of pittsburgh actually didn't let me go see the one at the + +01:58:47.640 --> 01:58:53.960 +beginning of the pandemic and uh we got in a big fight about it actually uh i wanted to go camping + +01:58:53.960 --> 01:58:58.920 +in tevisi and see it and he wouldn't allow me to do it so this this is one i'm not gonna miss i + +01:58:58.920 --> 01:59:04.040 +just hope that i hope that we have cloudless day on that day okay see you later guys thank you + +01:59:04.040 --> 01:59:06.760 +i'll see you tomorrow + +01:59:23.960 --> 01:59:26.280 +you + diff --git a/twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/README.md b/twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/README.md new file mode 100644 index 0000000..694197d --- /dev/null +++ b/twitch/2103069234 (2024-03-27) - We are Governed by Stories VENTER II -- (26 Mar 2024)/README.md @@ -0,0 +1,5 @@ +# We are Governed by Stories VENTER II -- (26 Mar 2024) -- Gigaohm Biological High Resistance Low Noise Information Brief + +## Streams +- https://twitch.tv/videos/2103069234 +