WEBVTT

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I'm afraid that the latest data tells us that we're dealing with essentially a worst-case

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that the latest data tells us that we're dealing with essentially a worst-case.

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The latest data tells us that we're dealing with the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data tells us that the latest data

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One priority.

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I think truth is good for kids. We're so busy lying, we don't even recognize the truth no more than society. We want everybody to feel good.

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That's not the way life is.

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But you can tell if someone's lying, you know, you can sort of feel it in people.

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And I have lied.

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I'm sure I'll lie again.

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I don't want to lie, you know, and I don't think I'm a liar.

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I try not to be a liar.

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I don't want to be a liar.

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I think it's like really important not to be a liar.

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I'm not sure exactly who he is.

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His name is J. J. Cooley, and I believe he's a consultant for CHD, and I believe he has

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a PhD in some sort of scientific discipline from what I understand.

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I don't want to be a liar, but I don't want to be a liar, and I don't want to be a liar.

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I don't want to be a liar, but I don't want to be a liar, and I don't want to be a liar.

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Good morning, everyone.

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This is Gigo.

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I'm biological.

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We're still working on the same enchantment.

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We're still trying to build the knowledge necessary to dispel it.

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Thank you very much for joining me this morning, wherever you are, whenever time it is, wherever

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you are.

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Thank you very much for being here.

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It is greatly appreciated.

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Thank you very much for joining me this morning, and thank you very much for joining me this

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morning, and thank you very much for joining me this morning, and thank you very much for

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joining me this morning, and thank you very much for joining me this morning, and thank

05:52.280 --> 05:57.280
you very much for joining me this morning, and thank you very much for joining me this

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morning, and thank you very much for joining me this morning, and thank you very much

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for joining me this morning, And thank you very much for joining me this morning, And

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thank you very much for joining me.

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And thank you very much for joining me this morning, and thank you very much for joining

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me this morning, and thank you very much for joining me this morning, and thank you very

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but you don't say that the play that they enacted is a lie,

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then essentially you're not questioning anything.

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If you say that everybody in the government is a traitor,

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but you don't question the laws that they pass,

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then what are you really questioning?

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And that's what they've gotten us to do

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with all of these people.

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That's what they've gotten to do with all of you.

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That's what we seem not to be able to show each other.

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That's not, it seems to be very hard to teach.

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You don't understand Mr. Brown, the bottom.

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When people decide to lie together,

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the results are absolutely awful.

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And these people who are agreeing not to question the faith,

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no matter how they, whatever else they question,

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I would argue are part of this.

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And I know a lot of people don't wanna hear that,

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but if you're just saying that there are bad guys out there,

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well, yeah, great, thanks for the update.

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I can see that.

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But if you're not calling the vaccine schedule

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what it is like every day and twice on Sunday,

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then I don't think you're really fighting.

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I think you're just dancing around the main problem.

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The very easy thing, the lowest hanging fruit,

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the thing that is backed by our history

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is backed by history that we can still accurately trace.

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And I think that's what's most important right now.

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If we continue to allow these charlatans

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to tell us the story that they wanna tell,

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then the truth will never be available to our children.

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And they will never get the power

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that probably are only our grandparents had,

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which is really sovereignty over our bodies

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and what we do with our future.

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I think we have been fooled into believing

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we've been in control of very much for our entire lives.

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Not everybody's parents were bad guys in this.

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Not all the bad guys have bad parents.

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Not everybody in this is part of the illusion.

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Wittingly.

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Sometimes it is, hello in the Philippines.

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Goodness sakes, wow.

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I hope that you have started to become someone

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like this guy in the red there,

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able to save people with a more rudimentary

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and simple understanding of things,

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able to understand the subtleties

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of how what looks to be very easy is actually very difficult.

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I hope you've been able to help someone else

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learn to surf this wave.

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I hope that we have helped some of you

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that GigaOM Biological, which is really not just me,

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but it's also my family that enables this to happen

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and all the people that were on that first screen

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that support this work.

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I hope that maybe some of you have been helped

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by GigaOM Biological to stay focused on the biology,

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to not take their bait on television

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and to love your neighbors irrespective of what's up.

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The reason why we are here every day often is possible

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and the reason why May is going to be another shift

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in terms of output is because people financially

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contribute to the work they subscribe

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and also viewers spread the word.

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That's the most important part.

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How many people work very hard,

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not doing anything else,

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but trying to share the screen.

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There's this guy named Soothe Spider

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who's working on ways to try and make it easier

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for me to put transcripts up on substack,

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which you might actually think would be pretty easy.

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I mean, if you look at some of these substack authors

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can put three or four articles out in a day

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and I find it very difficult having a video,

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having a transcript and still find it difficult

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to clean it up enough to get it on substack

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in a satisfactory manner that I think is worth somebody

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clicking on and that's what I find most extraordinary

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whereas someone else with a farm or maybe two farms

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and horses and emus can put out something

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like 17 substacks every two weeks

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and between 20 and 30 pages a day

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on average during the week.

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This is just extraordinary stuff

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and this extraordinary illusion is only sustained

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through your active participation.

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If you actually believe that people can emu farm

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and hand breed horses and tour the world in hot tubs

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while fighting a, leading a resistance, global resistance

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from the foot of the Shenandoah National Forest, I'm sorry

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but maybe you should buy an igloo in Arizona too.

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And this illusion, like I'm saying, is only sustained

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through your active, if you believe that these people

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are real and that their output is all just them

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and that these, these substacked authors

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are just covering what they want to cover

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and nobody's telling them what to do again.

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You're sustaining the illusion.

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If you believe that a lot of these groups

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that stood in front of fake microphones in 2020

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and we're trying to point out something,

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we're working for our best interest.

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I think you're just mistaken.

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I think you've been fooled by the same machinery

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that fooled a lot of us in the very, very beginning.

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And that's the danger really.

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Hope I didn't just do a bunch of copyrighted music there.

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I'm not really sure what that's all about there.

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Mike puts some stuff in there.

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A lot of this stuff is copyrighted

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but the way that he mixed it in, this guy.

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Mike Vandenberg, mixed date.

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That's the, Mike Vandenberg is no longer with us.

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He's a friend of mine from Amsterdam,

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my time in the Netherlands.

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He was a really great guy, real nice dude.

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He was a DJ and so that actually made him

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a very cool connection because every once in a while

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you would find out that,

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Michael, Mike Vandenberg,

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I mean, Michael is his Dutch name.

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He, you know, you might find out

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that he was at a party or something

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and I know one particular friend of mine

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took a lot of advantage of that much more than I did

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but that's actually who I met Mike through.

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And then, yeah, I just realized that

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what a funky opening music I could create

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from Mike's mixed tape.

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So anyway, I'm pouring a little coffee

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on the curb for my friend, Mike.

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Anyway, this is Gigo and Biological,

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High Resistance, Illinois's information brief,

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brought to you by a biologist.

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We are coming to you live from Pittsburgh, Pennsylvania

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and here it is approximately 10.30 in the morning

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on the 30th of April.

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And it's a cloudy skies

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and the temperature is incredibly warm.

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We always seem to skip spring in Pennsylvania.

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We go right from freezing every morning to,

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I can't stand the heat.

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And that's me making a little bit of a joke.

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I gotta be very honest with you.

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Pennsylvania is really one of the better places

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to live in America, I've gotta say.

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It's gotta be because the winters are easy.

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The summers have about two weeks of pain

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and the rest is, you know, okay, it's humid,

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but I mean, I've been other places.

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I know that this is not humid.

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It's more humid because we've got a lot of grass around

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and a lot of blacktop around, but it's not like Texas.

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It's, you know, it's not that hot and it's not that humid.

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It's not like Tennessee.

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I've been to Tennessee in times

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where I really wanted to off myself.

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And so Pennsylvania has been a really wonderful surprise

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coming here just because this was the one place

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that would give me a faculty position.

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My family has been very blessed to be in Pennsylvania

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and to be in Pittsburgh where at least half the people here

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have their head on straight.

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And so it is my pleasure to be here this morning.

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I'm really liking this 10-10,

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although I'm late this morning.

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It is a really nice time to be streaming.

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It's still cool air coming in the back door

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and blowing out the front of the garage.

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And so welcome to the show.

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Intramuscular injection of any combination of substances

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with the intent of augmenting the immune system is dumb.

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Transfection in healthy humans is criminally negligent

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and that's actually a well-known fact

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or should have been a well-known fact

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to most faculty members in the United States of medical schools

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and biology faculty all around the world really.

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And RNA cannot pandemic is something

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that even the most sophisticated

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and dogmatic bench biologist and chemist

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or anybody that rudimentary rudimentarily understands

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the basics of the DNA to RNA to protein dogma.

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Should be able to understand what I'm trying to get at

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when I say RNA cannot pandemic.

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I think it's a better way of saying that an RNA molecule

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is not a pattern integrity.

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And even if you wrap up an RNA molecule

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with a few enzymes, I don't think it constitutes

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a pattern integrity.

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And so that's really at the heart of this.

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And once you start to see it for what it is,

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you will start to realize that they have lied to us

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about a lot of the fundamental biology

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that they purport underlies the principle of a pandemic,

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the idea of a pandemic.

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And our current hypothesis remains pretty similar

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to what it has been over the last,

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it's evolved a little bit over the last six months.

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If you're joining us for the first time here

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at Gigome Biological, we pretty much have come

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to the conclusion that the who declared a pandemic

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of a dangerous novel virus.

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And then through the use of nonspecific PCR testing,

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in combination with a background,

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which was not a clean background,

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but whether it was a hot background,

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full of noise that these nonspecific tests

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could result in positivity.

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And then financial incentives that were added

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on top of this in certain places around the world,

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United States being one of them.

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Certain incentives where if you treated someone

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for this new disease,

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then there would be no possibility of malpractice.

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And so there were not only financial,

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but legal incentives in certain places like New York,

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where these mass casualty events really,

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New York is a shining,

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these shining example because there is no other example

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with the extent to which like a bomb went off

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in three and a half or four weeks

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where 20,000 people are purported to have died

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in that timeframe.

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In a system that's used to dealing with, let's say,

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1,000 bodies a week,

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now had to deal with four, five or 6,000 bodies a week.

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It's very unusual for there not to be a busload

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of traumatized healthcare workers in New York City.

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It's unusual that there isn't a concrete memorial

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with all their names on it.

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And that glaring omission is just kind of the tip

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of the iceberg.

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It should just be the light underneath the door

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that makes you curious as to what's on the other side

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and the little shadows moving like,

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oh, wow, what's going on in there?

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That's how quickly you should be interested in.

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Wow, let me, let's revisit what happened in New York City

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to understand how it is that we were convinced

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that this little thing here happened at the beginning.

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You can barely see my arrow

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when it's up here on the small screen.

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But that little blip right there at the beginning,

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which is up here also is actually

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what the sage group in the UK also used to model

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their projections in the UK based on.

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And so the trick here to understand is that RNA virology

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is not some high fidelity.

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We've been monitoring these things

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like watching the different populations of birds,

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wax and wane over the last 20 years

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and we understand the ecology of these things.

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That's not how it worked at all.

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They were very elusive.

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They're very difficult to culture.

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They're very difficult to follow.

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They always have to look at proxy signals

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rather than direct measurements.

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Essentially correlates of virology

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rather than like they use correlates of immunology.

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That's how extraordinary these things are.

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And that's how extraordinary this illusion has become.

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And because no one questions it,

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because no one wants to question Nobel Prize winners

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because no one really is interested in working on something

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that you can only understand by proxy.

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The only people who have gone into this

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and had any success at it seem to have been

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artificially elevated, hard officially propped up.

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People like Ralph Barrick.

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Ralph Barrick is probably a pretty legitimate molecular

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biologist who got co-opted into participating

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in this illusion.

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That's why a lot of times he seems uncomfortable

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in doing what he's doing.

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He hasn't gotten a Nobel Prize for any of the things

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that he's done like David Baltimore got a Nobel Prize

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for finding reverse transcriptase.

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But I'll come to none of these people got a Nobel Prize

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for finding RNA-dependent RNA polymerase.

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Could it be because we also have RNA-dependent RNA polymerases?

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It would be very strange if we did

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because for the last 15 years or so,

21:01.380 --> 21:04.320
we've been using the presence of an RNA-dependent RNA

21:04.320 --> 21:07.040
polymerase to indicate the presence of a coronavirus

21:07.040 --> 21:08.320
or another RNA virus.

21:08.320 --> 21:09.640
Isn't that strange?

21:11.920 --> 21:14.080
I don't know if you've done your due diligence or not

21:14.080 --> 21:15.640
in the last two days of the homework

21:15.640 --> 21:17.880
that I subconsciously assigned.

21:19.400 --> 21:21.480
But if you've gone to PubMed and you've looked,

21:21.480 --> 21:24.680
you've probably found some evidence for us having our own

21:24.680 --> 21:26.560
RNA-dependent RNA polymerases,

21:26.560 --> 21:30.160
but they're only associated with a very small,

21:30.160 --> 21:34.760
you know, niche mechanism of interfering RNA

21:34.760 --> 21:36.320
or some other thing like that.

21:37.160 --> 21:42.000
We are still being told, and I think this is something

21:42.000 --> 21:44.560
that the last few days have really done very well

21:44.560 --> 21:46.120
that our biology is very simple

21:46.120 --> 21:50.760
and all the extravagant, crazy biology is found in viruses.

21:50.760 --> 21:52.600
And so we've got to fight against them.

21:52.600 --> 21:54.640
We need drugs to fight against them.

21:56.800 --> 21:59.720
The goal is for us never to understand

21:59.720 --> 22:02.120
to what extent this biology has to do

22:02.120 --> 22:04.000
with understanding ourselves,

22:04.080 --> 22:07.120
has to do with their quest to understand ourselves,

22:07.120 --> 22:11.440
to somehow transcend our sacredness.

22:13.200 --> 22:15.320
They want you to believe it's all just public health

22:15.320 --> 22:16.320
and pathogens.

22:17.520 --> 22:20.320
But it's never been about public health and pathogens.

22:20.320 --> 22:22.960
It's always been about what the Human Genome Project

22:22.960 --> 22:23.800
has been about.

22:23.800 --> 22:28.800
It's always been about what even some of the nuclear

22:30.360 --> 22:33.240
weapons programs and nuclear energy actually

22:33.240 --> 22:37.600
were also very, those interests were all closely intertwined

22:37.600 --> 22:40.680
with the idea of using nuclear energy to influence

22:40.680 --> 22:44.960
or even accelerate evolution because they thought

22:44.960 --> 22:46.200
that, well, Darwin was right.

22:46.200 --> 22:48.360
It's just random mutations.

22:48.360 --> 22:52.600
And so if we used nuclear energy to accelerate

22:52.600 --> 22:54.960
the mutation rate, maybe we could accelerate

22:54.960 --> 22:56.160
human evolution.

22:57.400 --> 22:58.720
That's what they thought.

22:59.720 --> 23:04.720
These same people who told us what AIDS is

23:04.720 --> 23:06.600
and told us what tumor viruses are

23:06.600 --> 23:08.720
and told us what retroviruses are.

23:10.600 --> 23:15.040
All the same, it's all the same chain of teacher and student.

23:15.040 --> 23:19.160
It's all the same chain of ideas passed down

23:19.160 --> 23:22.920
from inspiring mentor to inspired student.

23:24.120 --> 23:26.320
And you can hear it in all of them.

23:27.320 --> 23:32.320
Paul Offit talks about his mentors and who are they?

23:32.320 --> 23:33.320
Who does he look up to?

23:33.320 --> 23:34.320
Think about that.

23:40.320 --> 23:41.600
You want help thinking about that?

23:41.600 --> 23:45.320
Check out Mark Hewson-Tonic Mark Koolack on Mark.

23:45.320 --> 23:48.520
Mark does Hewson-Tonic ITS.

23:48.520 --> 23:49.680
It's Hewson-Tonic Live.

23:49.680 --> 23:52.640
He got another strike yesterday for one of his shows.

23:52.640 --> 23:55.960
I find it really strange, quite frankly,

23:55.960 --> 24:00.800
that Mark Hewson-Tonic gets a strike on his podcast.

24:03.880 --> 24:07.200
But this video about the spike protein

24:07.200 --> 24:09.680
being an engineered pre-on protein

24:09.680 --> 24:12.680
by Veijon Medicine Shorts

24:12.680 --> 24:16.400
by Dr. Philip McMillan has been up for two months

24:16.400 --> 24:17.900
and that gets no strike.

24:18.960 --> 24:20.440
Does that mean it's true?

24:21.440 --> 24:25.440
And Mark talking about people

24:25.440 --> 24:30.440
and who they talked about in their obituaries

24:30.440 --> 24:32.440
or whatever, that's...

24:36.440 --> 24:40.440
I think you got to see this for what it is, ladies and gentlemen.

24:40.440 --> 24:42.440
What we've been trying to describe

24:42.440 --> 24:46.440
is a very slow role of a narrative.

24:46.440 --> 24:49.440
A narrative that had a lot of moving parts.

24:50.440 --> 24:53.440
And the goal was to make sure that the entire globe,

24:53.440 --> 24:55.440
and that's a big number,

24:55.440 --> 24:58.440
the entire globe basically bought the premise

24:58.440 --> 25:03.440
that a novel virus was discovered in 2020

25:03.440 --> 25:07.440
and circled the globe for five years,

25:07.440 --> 25:11.440
causing death, destruction, and economic chaos.

25:12.440 --> 25:15.440
What the details are about what you accept

25:15.440 --> 25:18.440
with regard to that story are actually irrelevant.

25:19.440 --> 25:21.440
Because if you accept that story,

25:21.440 --> 25:24.440
then this hypothesis or anything related

25:24.440 --> 25:27.440
to the idea that a national security operation

25:27.440 --> 25:30.440
was executed in order to convince you

25:31.440 --> 25:34.440
that an RNA pandemic has occurred.

25:34.440 --> 25:36.440
You will never get there.

25:36.440 --> 25:39.440
And that was their job that remains their job.

25:39.440 --> 25:42.440
And I think it's really important for people to understand

25:42.440 --> 25:45.440
that this is not some kind of secret thing.

25:46.440 --> 25:50.440
We are very, very close to being exactly on script

25:50.440 --> 25:52.440
with this thing right here,

25:54.440 --> 25:56.440
which is the sparse pandemic.

25:58.440 --> 26:01.440
The plan of the sparse pandemic, right?

26:01.440 --> 26:03.440
Let me just see if I can get over there.

26:03.440 --> 26:06.440
And let me do this and bookmarks

26:06.440 --> 26:09.440
and I can hide the bookmark toolbar

26:09.440 --> 26:11.440
and then I can go over here.

26:12.440 --> 26:14.440
So this is the sparse pandemic.

26:15.440 --> 26:18.440
This is that thing that you can find on the internet

26:18.440 --> 26:20.440
and it is something that was done,

26:20.440 --> 26:23.440
I believe, by Johns Hopkins

26:23.440 --> 26:26.440
and maybe also the Rockefeller,

26:26.440 --> 26:28.440
Johns Hopkins, Johns,

26:28.440 --> 26:30.440
a Johns Texas state,

26:30.440 --> 26:32.440
recommended citations, Johns Hopkins,

26:33.440 --> 26:35.440
Bloomberg School of Public Health.

26:35.440 --> 26:37.440
Maybe it's not Rockefeller.

26:37.440 --> 26:38.440
I don't know.

26:39.440 --> 26:43.440
So the whole idea is that something happens in the Philippines

26:43.440 --> 26:46.440
where there's hypothetical scenario

26:47.440 --> 26:50.440
and it's the Philippines and I think it's pigs that get sick.

26:50.440 --> 26:52.440
Let's just go through this really quick

26:52.440 --> 26:54.440
and then I'll show you what I wanted to say

26:55.440 --> 26:57.440
or what I wanted to point out.

26:57.440 --> 26:59.440
So you can go through this document.

26:59.440 --> 27:00.440
If you haven't gone through this document,

27:00.440 --> 27:01.440
I don't even know where you've been.

27:01.440 --> 27:03.440
We did this document like four years ago.

27:03.440 --> 27:05.440
We started this document.

27:05.440 --> 27:07.440
There might even be a bike ride about this document,

27:07.440 --> 27:08.440
but I don't think there is.

27:08.440 --> 27:10.440
It would have been a walk in the woods.

27:11.440 --> 27:12.440
And so, you know,

27:12.440 --> 27:15.440
I didn't even think I really needed to cover this document very much

27:15.440 --> 27:17.440
because I think everyone has done it.

27:17.440 --> 27:20.440
But if you haven't, I mean, please read it.

27:20.440 --> 27:23.440
I mean, it's not meant to be a funny thing.

27:23.440 --> 27:25.440
They use this as a gaming.

27:25.440 --> 27:27.440
It's a game scenario.

27:27.440 --> 27:30.440
It's trying to understand what might happen

27:31.440 --> 27:35.440
so we can understand what might be appropriate solutions for it.

27:36.440 --> 27:37.440
And so let's see.

27:40.440 --> 27:42.440
So then it's a hoofed animal.

27:42.440 --> 27:45.440
It's a coronavirus outbreak in hoofed animals.

27:46.440 --> 27:49.440
And then I think what they do is they hired a company

27:49.440 --> 27:50.440
to produce the vaccine.

27:50.440 --> 27:51.440
This is a great story.

27:52.440 --> 27:53.440
And then they produce the vaccine.

27:53.440 --> 27:55.440
And I think after they produce the vaccine,

27:55.440 --> 27:58.440
although it hurt some animals,

27:58.440 --> 28:03.440
the virus jumps out of the animals into people.

28:03.440 --> 28:06.440
So I don't think they make much of a story about the fact

28:06.440 --> 28:08.440
that the vaccine did it or something like that,

28:08.440 --> 28:09.440
but it's not not implied.

28:09.440 --> 28:13.440
It's definitely something that when you read the document,

28:13.440 --> 28:16.440
you can think, oh, wow, did the vaccine cause that?

28:16.440 --> 28:19.440
And it's an interesting parallel to what Heritbund and Bosch

28:19.440 --> 28:21.440
is now pushing with regard to the,

28:21.440 --> 28:22.440
to the,

28:22.440 --> 28:25.440
the transfection that these people keep calling a vaccine.

28:25.440 --> 28:29.440
The transfection has caused SARS-CoV-2 to do.

28:34.440 --> 28:35.440
That was my second cup of coffee.

28:35.440 --> 28:36.440
We're doing great this morning.

28:37.440 --> 28:44.440
And so then they have to use emergency use authorized drugs.

28:44.440 --> 28:47.440
And there's some drugs that work and some don't.

28:47.440 --> 28:49.440
And there's, there's misinformation.

28:49.440 --> 28:51.440
And so it's very, very familiar.

28:53.440 --> 28:55.440
Some people reject the untested drugs.

28:55.440 --> 28:59.440
You know, it's all this, what, what kind of hypothetical debates,

29:00.440 --> 29:05.440
hypothetical, you know, they're looking at pre-crisis partnerships

29:05.440 --> 29:06.440
that could have avoided these things.

29:06.440 --> 29:09.440
So they're, they're sort of implying that there are ways

29:09.440 --> 29:13.440
that we can prepare for it, but I find most extraordinary

29:13.440 --> 29:16.440
about where we are now, especially with regard to the,

29:16.440 --> 29:18.440
to the video that I just referenced,

29:18.440 --> 29:19.440
which hasn't gotten a strike.

29:19.440 --> 29:22.440
It's just up there saying that the spike protein is a,

29:22.440 --> 29:25.440
is a laboratory designed pre-on like,

29:25.440 --> 29:27.440
in-capacitation agent.

29:30.440 --> 29:34.440
That video's up, but Mark Gulak gets a strike.

29:35.440 --> 29:38.440
If we go to just find something here,

29:38.440 --> 29:40.440
I don't know if I, if I got to get my head out of the way.

29:40.440 --> 29:41.440
Yeah, here we go.

29:42.440 --> 29:45.440
And I got to get the mouse up there and then we go here.

29:46.440 --> 29:49.440
I'm just going to put in neuro because I know what their talk,

29:49.440 --> 29:50.440
I know where this is.

29:50.440 --> 29:52.440
I've done this video or done this.

29:52.440 --> 29:57.440
Done this document many times, I know how to get and navigate it.

29:57.440 --> 30:01.440
So in chapter 17, which is, you know, you got to get there.

30:01.440 --> 30:03.440
It's a long, it's a page 59.

30:03.440 --> 30:06.440
They start talking about vaccine injury.

30:06.440 --> 30:11.440
So this, this scenario goes from the outbreak all through the

30:11.440 --> 30:16.440
response and all through the ramifications of a rushed,

30:16.440 --> 30:21.440
uncoordinated, um, blind response, right?

30:21.440 --> 30:27.440
And so all the scenarios that are now being pushed by teamwork case scenario

30:27.440 --> 30:34.440
and by the fake dissident movement are precisely codified here in some mirror image.

30:35.440 --> 30:37.440
I mean, it's just plain and simple.

30:37.440 --> 30:38.440
You don't like it.

30:38.440 --> 30:42.440
The existence of this document then is what it is.

30:42.440 --> 30:43.440
Is it pre programming?

30:43.440 --> 30:45.440
Then what do you want to call this document?

30:46.440 --> 30:50.440
This is the bureaucracy preparing to execute what we just experienced.

30:50.440 --> 30:56.440
What we are experiencing now is the next stage of it has not stopped.

30:56.440 --> 31:02.440
And in this part of the document, they are detailing how a certain small fraction

31:02.440 --> 31:06.440
of people are finding out that, wow, there is some legitimate.

31:06.440 --> 31:11.440
There are some vaccine injuries and lo and behold, what's happening right now in

31:12.440 --> 31:16.440
America, what's happening right now is a group of people are being elevated

31:16.440 --> 31:22.440
specifically to say that there are vaccine injuries.

31:22.440 --> 31:27.440
And even co-opting people like Denny Rancor have been speaking out since

31:27.440 --> 31:33.440
2020 and taking only his part of the story that says 17 or 18 million people

31:33.440 --> 31:34.440
were killed by the shot.

31:34.440 --> 31:39.440
Not the part of his story that says said since 2020 that there's no evidence

31:39.440 --> 31:40.440
that it was spread.

31:40.440 --> 31:44.440
He's been publishing that longer that he's been publishing the damage of the

31:44.440 --> 31:48.440
vaccine and that makes sense because before the vaccine came out, it was

31:48.440 --> 31:52.440
already obvious to him and others that something wasn't right about the

31:52.440 --> 31:53.440
narrative they were telling.

31:53.440 --> 31:58.440
And it turns out it was about it was about lying about murder.

31:58.440 --> 32:00.440
It was about falsifying records.

32:00.440 --> 32:02.440
It was about fraudulent death dates.

32:02.440 --> 32:04.440
It was probably about all of that.

32:05.440 --> 32:14.440
And so this is the forward facing document of such a exercise.

32:14.440 --> 32:19.440
This is the unclassified public part of an exercise like this.

32:19.440 --> 32:22.440
What is the classified part?

32:22.440 --> 32:25.440
What's the classified part that would be like the G.

32:25.440 --> 32:29.440
or Dono presentation, but for people with a lanyard?

32:29.440 --> 32:33.440
Because that's what you've got to read when you read a document like this.

32:33.440 --> 32:37.440
Not read a document like this and say, oh, wow, now we know.

32:37.440 --> 32:42.440
No, you read this document like it's the brochure.

32:42.440 --> 32:45.440
The paid content is not in here.

32:45.440 --> 32:50.440
And so if the brochure says seven times that vaccine injuries are going to be

32:50.440 --> 32:54.440
neurological symptoms similar to those seen in the livestock exposed to the

32:54.440 --> 32:58.440
GMI vaccine when you go all the way back to the beginning, you get

32:58.440 --> 33:02.440
encephalitis and brain swelling and all kinds of other neurological

33:02.440 --> 33:06.440
things that are damaging and they killed all those animals to avoid the

33:06.440 --> 33:09.440
detection in the animal vaccine.

33:09.440 --> 33:13.440
What an interesting story that is, isn't it?

33:13.440 --> 33:15.440
Because our human trials were all screwed up.

33:15.440 --> 33:20.440
Our human trials used a different vaccine according to these people.

33:20.440 --> 33:22.440
It was process one versus process two.

33:22.440 --> 33:27.440
So there are mirrors in every part of the story right up until we get to

33:27.440 --> 33:33.440
preons and amyloidosis and the amyloidogenesis and the sudden synonymous

33:33.440 --> 33:38.440
words that all mean the same thing that proteins fold from alpha helices to

33:38.440 --> 33:42.440
beta pleated sheets and that sucks.

33:42.440 --> 33:45.440
Even though before the pandemic,

33:45.440 --> 33:51.440
alpha helices and beta pleated sheets and beta helices were all just kind of

33:51.440 --> 33:59.440
standard run of the mill generalized terms for shapes that proteins can take.

34:01.440 --> 34:06.440
Ladies and gentlemen, we are being bamboozled from the highest level in the

34:06.440 --> 34:12.440
highest level of manner, you know, like the fifth generation warfare that

34:12.440 --> 34:15.440
Robert Malone told us will never be able to figure out.

34:15.440 --> 34:18.440
He actually said that on stage a few times.

34:19.440 --> 34:24.440
Go find the videos where instead of talking about biology or his patents or

34:24.440 --> 34:27.440
what he understands or doesn't understand about the shot that he took

34:27.440 --> 34:29.440
or didn't take.

34:29.440 --> 34:32.440
And he's given these fifth generation warfare talks.

34:32.440 --> 34:35.440
One of the main talking points is you're never going to figure it out.

34:35.440 --> 34:36.440
And why not?

34:36.440 --> 34:42.440
Because it's people agreeing to lie together.

34:42.440 --> 34:45.440
Even lie about each other together in coordination, you know,

34:45.440 --> 34:52.440
like be opposers opposing each other in opposition.

34:52.440 --> 34:53.440
Oh, he's not.

34:53.440 --> 34:54.440
He's a fake guy.

34:54.440 --> 34:55.440
Yeah, yeah.

34:55.440 --> 34:56.440
Right.

34:56.440 --> 34:57.440
He's my nemesis.

34:57.440 --> 34:59.440
See, he was on 60 minutes.

34:59.440 --> 35:00.440
Yeah.

35:00.440 --> 35:01.440
Right.

35:01.440 --> 35:05.440
That's how deep this theater goes.

35:05.440 --> 35:10.440
And all I would need to do is sit down with a bunch of pictures and start typing

35:10.440 --> 35:15.440
what I know about each of them in every one of the four years that we've been in.

35:15.440 --> 35:20.440
20, 21, 22, 23.

35:20.440 --> 35:24.440
And you would see a picture so clear.

35:24.440 --> 35:28.440
You'd need sunglasses to look at it.

35:28.440 --> 35:35.440
It is so blatantly obvious who was acting and who was trying to figure out what

35:35.440 --> 35:41.440
and the hell's going on, who was being lied to and manipulated and who was doing the

35:41.440 --> 35:48.440
lying and the manipulating because the only rational reaction to lying and manipulating

35:48.440 --> 35:52.440
is frustration and fear and panic.

35:52.440 --> 35:56.440
Exactly what you've seen me do a lot of times unwittingly.

35:56.440 --> 35:57.440
It just lose it.

35:57.440 --> 36:04.440
You, you, you panic because you tried to explain to people that you didn't want to see this

36:04.440 --> 36:05.440
in the NFL.

36:05.440 --> 36:09.440
You don't want to be telling people that they're, they're part of something.

36:09.440 --> 36:16.440
But look at this document, understand that this is a brochure that the real plan isn't

36:16.440 --> 36:17.440
published.

36:17.440 --> 36:24.440
And that the real plan involves coordinated lying where the limited spectrum of debate

36:24.440 --> 36:33.440
is actually two shepherds that argue about what's important and both arguments are wrong.

36:33.440 --> 36:38.440
They're the wall of the cage.

36:38.440 --> 36:43.440
And as we move forward and more and more people argue about the adulteration of the double

36:43.440 --> 36:50.440
stranded DNA or argue that the spike protein is somehow a designer thing that can cause,

36:50.440 --> 36:56.440
you know, amyloidosis to occur or cause preonogenesis.

36:56.440 --> 37:06.440
Big words for, for protein folding in a maladaptive way with very little basis in mechanistic

37:06.440 --> 37:08.440
understanding.

37:08.440 --> 37:16.440
In other words, preonogenesis, amyloidosis, these are processes that sound as though there's

37:16.440 --> 37:19.440
a high fidelity understanding underlying them, but they're not.

37:19.440 --> 37:23.440
They're actually words that indicate very little understanding.

37:23.440 --> 37:33.440
And so they use those words to hand wave because as they've moved through this space, they've

37:33.440 --> 37:41.440
had to slowly navigate around this one problem that heretofore structure equals function.

37:41.440 --> 37:48.440
Structure equals the amino acid sequence in combination with the ribosomal processing.

37:48.440 --> 37:50.440
That's it.

37:50.440 --> 37:53.440
We don't have any explanation beyond that.

37:53.440 --> 37:59.440
Somehow or another, this RNA goes through and you get a protein on the other side.

37:59.440 --> 38:02.440
Most of the time that protein comes out in this shape.

38:02.440 --> 38:10.440
If you change some of these bases in these codons to silent mutations, you can actually

38:10.440 --> 38:13.440
get misfolding that leads to rare genetic disorders.

38:13.440 --> 38:18.440
We don't understand how that occurs.

38:18.440 --> 38:25.440
And that was on the front of science magazine in 2011 with a cartoon mouth that had a zipper

38:25.440 --> 38:26.440
through it.

38:26.440 --> 38:31.440
It was the secret that nobody wanted to talk about that we didn't understand how silent

38:31.440 --> 38:40.440
mutations that don't change the amino acid can be so highly coordinated, highly correlated

38:40.440 --> 38:42.440
with genetic disorders.

38:42.440 --> 38:46.440
It didn't make sense to us.

38:46.440 --> 38:53.440
And it still really doesn't.

38:53.440 --> 39:00.440
And so the level of understanding that is implied is a lie.

39:00.440 --> 39:07.440
And it is a lie that falls right in line with a narrative about a novel virus that we had

39:07.440 --> 39:14.440
to respond to, but we responded to rather clumsily and now have to respond to the response of

39:14.440 --> 39:15.440
it.

39:15.440 --> 39:17.440
We have to mitigate the damage from the response.

39:17.440 --> 39:20.440
We have to recover from that.

39:20.440 --> 39:27.440
And nowhere in this document that they talk about the loss of liberty or sovereignty and

39:27.440 --> 39:34.440
the ramifications of that, that's not in this document at all because this is a sales brochure

39:34.440 --> 39:37.440
for a plan.

39:37.440 --> 39:42.440
And the real plan isn't published, but the real plan was executed on us.

39:42.440 --> 39:49.440
The real plan involved people in front of the television, on social media with podcasts

39:49.440 --> 39:57.440
and it involved insiders that pretended to be whistleblowers and pretend insiders pretending

39:57.440 --> 40:04.440
to be whistleblowers, all of whom are participating in our governance through lies.

40:04.440 --> 40:05.440
That's where we are.

40:06.440 --> 40:12.440
And this spars thing where we go from, we could, how many times we can see the neurological

40:12.440 --> 40:19.440
symptoms similar among the livestock and then we'll go down to the next one.

40:19.440 --> 40:25.440
And while the FDA, CDC and other agencies were busy researching possible connections

40:25.440 --> 40:31.440
between Corovacs and reported neurological side effects, their efforts were continually

40:32.440 --> 40:36.440
undermined by epidemiological analysis produced by various non-governmental and individual

40:36.440 --> 40:43.440
groups, a popular science blogger, EpiGirl, began posting interactive maps of the incidents

40:43.440 --> 40:45.440
of COVID side effects.

40:45.440 --> 40:48.440
Oh, EpiGirl.

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Is that who Jessica Rose is?

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Is Jessica Rose just EpiGirl in this SARS-CoV-2 pandemic narrative?

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She's one of those people who could have called this thing transfection from the very

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beginning but hasn't.

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I mean, she's got like four degrees, right?

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Is it computational biology, biochemistry, immunology, virology, applied mathematics?

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I don't know what, when it's listed.

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I forget them all because it's just so many.

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But our first four or five publications are all making the mathematical simulations of

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the virology paper that she's in.

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And a lot of our papers and research has been funded by some NATO thing, which is I also

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really strange because I was working in NATO member countries and nobody ever said that

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I could apply for neuroscience grants that were funded by NATO.

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I never heard of that.

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And Jessica Rose also has not actually ever applied for or looked for a 10-year position.

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She's not actually an academic.

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She's done some postdocs but it doesn't seem like she's ever sought out a position and tried

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to establish a lab because that's what people do in science.

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And what I do in the Netherlands and failed and what I tried to do in partnership with

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somebody else in Pittsburgh and succeeded at until the start of the pandemic.

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Really?

42:33.440 --> 42:36.440
I mean, we were killing it.

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And we were helping other labs and collaborating with other labs.

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I mean, we had become kind of the go-to patch clamp physiology.

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You know, if you're having problems, go to them.

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If you need an experiment done, ask them.

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And we had partnerships with monkey labs and other labs with special transgenic mice.

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They were telling stories about it.

42:58.440 --> 43:04.440
And so I was doing really well and our lab was doing really well, even though.

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And so I was playing the game legitimately.

43:08.440 --> 43:12.440
I don't see the same thing in EpiGirl.

43:12.440 --> 43:19.440
In EpiGirl, I see someone who now is doing very well as what is essentially a sub-stack writer.

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And that's fine. I don't begrudge her that, but let's be realistic about what happened here.

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She was in these hot tub pictures.

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You know, she's been on stage with Robert Malone in multiple places.

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She's been in these places where I feel like the narrative has only been reinforced and very carefully curated.

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And with someone with so many degrees can only focus on a few things.

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And when she's actually called to speak, it's always about bears.

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It's never about the basic immunology.

43:57.440 --> 44:01.440
Can you tell us a little bit about the basic immunology of this stuff?

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It would be a very, very succinct presentation of ACE2 and all the other garbage that we were told from the very, very beginning.

44:09.440 --> 44:15.440
That hasn't changed at all in the mainstream narrative.

44:15.440 --> 44:19.440
I'm going to wake up right now, ladies and gentlemen. There isn't much time left.

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And I can only share this truth so many times.

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But they have planned this out.

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And don't get me wrong.

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There are lots of EpiGirls.

44:35.440 --> 44:38.440
Brett Weinstein is an EpiGirl.

44:38.440 --> 44:41.440
Brett Weinstein is just evolutionary girl.

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And they started them earlier. They planted them earlier.

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They did a stunt at a university, which was really a stunt, ladies and gentlemen.

44:49.440 --> 44:58.440
How was it a stunt? Because when I was, my contract was no longer extended because of, of COVID, I had no recourse.

44:58.440 --> 45:05.440
There's nothing that I could do. I was just a postdoc with a one year contract, tough shit.

45:05.440 --> 45:12.440
He, on the other hand, and his wife got a half a million dollar settlement, got to speak and complain about it in front of the Senate.

45:12.440 --> 45:26.440
Got a, got a pretty good start for their podcast that they now say that they lost their YouTube monetization because they let Robert Malone come on their stream and speak about how he had taken the vaccine to travel and try to avoid his long COVID.

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And that they told everybody if we could just get everybody to take Ivermectin and vuvoxamine for 30 days, we could get to zero COVID.

45:36.440 --> 45:41.440
And that focused hard lockdowns could get us to zero COVID.

45:41.440 --> 45:47.440
Sounds like EpiGirl to me.

45:47.440 --> 46:01.440
And once you realize that there's just been a bunch of EpiGirls around telling us all these things and now the EpiGirl people are telling us that there are going to be preons and they're, the preons are not just from transfection, which I could have told you.

46:01.440 --> 46:08.440
The protein misfolding and protein disorders and, and, and mitochondrial damage.

46:08.440 --> 46:11.440
This stuff we could have all told you is just the transfection.

46:11.440 --> 46:14.440
It's not specific to the spike protein yet for some reason or another.

46:14.440 --> 46:20.440
In 2020, there were already more than a few people who were sure that the spike protein was a neurotoxin.

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The spike protein was a neurotoxin.

46:22.440 --> 46:28.440
The spike protein was a neurotoxin.

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And I want to make this point every night, every day, any time and three times on Sunday, they seeded the narrative about the spike protein so that you would think that the average mouth breather that works at a gas station or at a 7-Eleven would think it makes pretty much sense then that picking that protein was a really bad idea for the shot.

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And that maybe if they don't pick that protein next time, transfection will be fine and whatever they do with mRNA was fine.

46:56.440 --> 46:58.440
It's just this spike protein.

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Why was it a nasty thing?

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And the funny thing is the same people are still saying that.

47:03.440 --> 47:06.440
They have not moved even a minute.

47:06.440 --> 47:11.440
I mean, they, they still say the spike protein is the only protein you have to worry about.

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They still say that the spike protein has these special things in it like a fear and cleavage site and that makes it magic.

47:19.440 --> 47:23.440
And they're all epigirls.

47:23.440 --> 47:25.440
They're team worst case scenario.

47:25.440 --> 47:36.440
They're there to control the narrative to make people ask a certain question that won't get them out of the loop to focus on a certain list of questions that won't get them out of the loop.

47:36.440 --> 47:43.440
How about right away at the beginning to make sure that you paid attention to the right people?

47:43.440 --> 48:00.440
Like what if I came out in March of 2020 and was convinced, absolutely convinced that the main thing that I was called to do was to oppose the people that thought 5G was important and oppose the people that thought they were no virus.

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What if that was my primary mission that I just had a, had a sign up behind me that says, we've got to fight the no virus people because they're idiots.

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And that, that we've got to fight the people that, that are opposed to 5G because they're idiots.

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Wouldn't that strike you as odd?

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That's not what I came out in. I came out against the, I came out the idea that previous immunity to whatever these things are is definitely better than whatever they're suggesting.

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And that we should open schools because kids were never vulnerable to whatever they were suggesting they were vulnerable to.

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And that somehow, some way that we were being lied to about this.

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And yes, it took me a long time to figure out how we were being lied to in layers by layers of coordinated liars.

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But once you start to tumble out of this, you tumble pretty fast and you realize when you hit the floor that holy cow, there aren't very many people down here.

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Ladies and gentlemen, this document, the centerforhealthsecurity.org has the spares pandemic scenario PDF.

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Read it, understand it, and realize that it is a mirror. It is a map of brochure of how this happened, a brochure of what they want you to believe happened.

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And we are still being led by our noses through this narrative, which takes us right into slavery for our grandchildren.

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And the people who are leading us, the people that are being put in front of us, they are willing participants at this point.

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There are no people who have not woken up to the idea that something drastically wrong happened in 2020.

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The people that have woken up like that are not being promoted anymore.

49:59.440 --> 50:18.440
And it is very important to realize that Kevin McCairn, the guy who has known that the spike protein is very, very bad that a billion people might die in worst case scenario that masks are great, that had the had the virus from a super spreader event in Korea

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and has been convinced that Andy Kaufman is the most terrible no virus person ever since March of 2020 has now been featured in life site news as a pre on expert and essentially quoted right alongside Kevin McCurnan, who's a genome sequencing expert.

50:43.440 --> 50:47.440
Who's now also talking about mitochondrial damage.

50:48.440 --> 51:00.440
And mitochondrial damage was known. It was expected to come not from the transfection, ladies and gentlemen, but from the lipid nanoparticle, the cationic lipids.

51:01.440 --> 51:19.440
And so it's very, very amazing to me how all of this is right in front of our faces, right there for us to take all these relationships between these people that Mark has been outlining over the last two years, or three years.

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These have been right there for the taking for any of these other people to find any of these other people to expound on and yet somehow or another they've all missed it.

51:27.440 --> 51:39.440
The supplementary pure oxygen has been there for any of these doctors or may never mind doctors that are also JD's and also PhD's to have found, but they didn't find it.

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They didn't see it.

51:44.440 --> 51:47.440
How can that be by accident?

51:48.440 --> 51:51.440
At this stage, it can't be by accident.

51:52.440 --> 51:56.440
This confusion was created on purpose.

51:57.440 --> 52:04.440
This pain was created on purpose and the distortion was created on purpose because it was a national security exercise.

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And a very fun brochure of that near related exercise is called the sparse pandemic scenario PDF.

52:15.440 --> 52:35.440
So, I think it's really important to understand that this is a national security event and that's what gives these people the confidence and the, again, can also coerce some of these people into telling the stories that they're telling and sticking to those stories.

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Now, I don't know what they were told. I don't know what they were threatened. They all may have been threatened or told or given different things.

52:44.440 --> 53:04.440
But I know that there are people who are really trying to fight for truth, trying to fight for America and trying to fight for their own country to be free of these globalists, to be free of these transhumanists, to be free of these people who think that nothing is sacred about human life.

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Except for their own, perhaps.

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So, let's see, where am I going here with this before I do the next slide. Oh, yeah.

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Already there.

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Now, I don't have any water down here because I couldn't find my friggin water bottle because I couldn't find my GoPro.

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I am going to start helping my wife livestream her yoga on YouTube.

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And so anyway, in helping set that up yesterday, I took my GoPro up there to make a secondary camera.

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And then as it goes with a guy like me, I carried it somewhere after I took it out of its mount up there to bring back downstairs to my room in the garage.

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And I don't know how, but I dropped it, put it down somewhere and for the life of me, I had to search the entire house this morning before I, of course, I found it.

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And where was it? It was underneath. Yeah, anyway, it doesn't matter. It's a little black cube and a little black cubes when you're angry and searching quite quickly are very hard to see, especially when they're right in front of your face.

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So anyway, I couldn't find my water bottle because I spent like 30 minutes trying to find my GoPro and then I probably won't even write anything down today because this lecture will be so bad, but I wanted to round off.

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I'm spelling his name wrong on purpose here because I don't care because I don't have any respect for these people who I think now are just masquerading as biologists.

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But I want you to see 10 years ago what the Baltimore classification would have looked like from his star student, Vincent Rassini, Rec and Yellow.

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And I want you to hear how he talks about viral genetics and how virology is done.

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These two short clips illustrate the mythology that I think we're fighting and the mythology that I think we need to.

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We need to dispel before the end of the year, so at least among our family and friends so that we start to make real progress with dispelling it around the earth.

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I know that sounds like an impossible, a ridiculous task for somebody in the back of their garage, but we each influence one person at a time.

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That's the best we can hope to be as far as greatness.

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I'm trying my best with my kids, and I'm also trying my best with giga home biological.

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I don't know that everybody can stream. Not everybody wants to stream.

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I don't know if I want to stream, but I feel like I'm a pretty decent teacher.

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And so that's why I feel like I'm going to keep teaching this stuff.

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If you like what I'm doing here, please remember that I'm only supported by people like you.

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If you can find a way to give 10 bucks a month, that would be really great.

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There's a place to do it on my website. You could also click on the PayPal link and set up to give $1 a month.

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I finally figured out how to make that an option there.

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And so no matter how little or how much you think you can give every month, please consider supporting the work because.

56:21.440 --> 56:28.440
I really feel as though the next couple months are really going to be key for us breaking this this summer.

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And fall.

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I hope to really start to put out something that I think people will find worth supporting.

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And I know there are more than 140 people that are already supporting me now and it's fantastic.

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I think we have 130 actually 132 subscribers or something like that.

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And there are also a few people that just give on their own every once in a while.

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And so we squeak by, but this month has been the worst.

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And this month has now been the hairiest for my family. I think we're pretty much really on fumes now so.

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We want to do more. We want to we want to give more. We want to put more work into this.

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But we're really doing as much as we can right now trying to keep track of.

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This narrative and put it together in a succinct way. And so you'll see it in May.

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What we've been working on behind the scenes and it'll become kind of obvious that there's something really.

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Unique here.

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Especially over the last few weeks, if you haven't been watching the stream regularly, we've made some really big breakthroughs and with the help of a few friends.

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Some of the haziness of this biology has been clarified for me just simply because I can now see clearly what they really understand and what they've been hand waving for a very long time and what they don't want you to talk about.

57:59.440 --> 58:09.440
And those few things that they don't really want to admit are the key things that I'm going to try and hone in on with regard to Vincent's two lectures here.

58:10.440 --> 58:16.440
So I'm going to escape out of this and I think I have them already popping up here. Yes, I do.

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First one.

58:19.440 --> 58:23.440
And these lectures are from 10 years ago and they are clipped.

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Which basically means that they are taken from an hour long lecture and they're both about 15 minutes long.

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I'm going to play them at regular speed, but I discovered something that you might not know.

58:35.440 --> 58:48.440
But when you're playing a YouTube video, you can hit the hit the pause button is the space bar, but I don't know if you knew this or not, but if you hold the space bar down, it will play at one and a half speed until you let go of it, which is kind of a thing.

58:48.440 --> 58:55.440
If you're trying to fast forward through a video, it's quite handy and I didn't know that because I'm not really big into keyboard shortcuts.

58:55.440 --> 59:08.440
And I know that somebody like my friend Ted on the other side of the planet would laugh because all those people that work on computers are know all the keyboard shortcuts and think that us people that don't use keyboard shortcuts are, you know, mouth breathers.

59:08.440 --> 59:12.440
So I figured out a new keyboard shortcut today.

59:12.440 --> 59:13.440
So I'm very proud of myself.

59:13.440 --> 59:14.440
Anyway, here we go.

59:14.440 --> 59:15.440
This is the first lecture.

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I believe this one is called.

59:18.440 --> 59:22.440
I should probably tell you what that one is called the Baltimore scheme.

59:23.440 --> 59:41.440
So we're going to look at the Baltimore scheme and remember that Vincent Rancin yellow is the guy that invented infectious clones with David Baltimore where they took an RNA virus and they made an RNA copy of it and that RNA was infectious and that was the first infectious

59:41.440 --> 59:51.440
clone that's a really significant thing because I'm arguing that infectious clones are the illusion of virology that what they want you to believe is that there are signals.

59:51.440 --> 59:54.440
There are signals like this in the wild.

59:54.440 --> 01:00:09.440
And they can grab them and grow them and study them but in reality they haven't been able to do that at all until they figured out how to convert them to DNA make a lot of DNA and then convert those by putting them on a cell culture to the RNA virus.

01:00:10.440 --> 01:00:23.440
And you're going to hear a description of this which is going to be very imprecise is going to use transformation and transfection a little differently than I do because it's 10 years ago, but I believe it's because he's obfuscating the truth.

01:00:23.440 --> 01:00:32.440
And so first the Baltimore scheme, then his description in the same course, I believe, of viral genomes and how we study them.

01:00:32.440 --> 01:00:44.440
And so you're going to find this I think very enlightening especially if you followed along with the David Baltimore lectures from a couple days ago because remember, the Baltimore lectures from a couple days ago are from 2018.

01:00:44.440 --> 01:00:51.440
And this lecture is from his student, but several years before that.

01:00:52.440 --> 01:01:10.440
So it's not like what you're going to see from your you're going to find very quickly that what David Baltimore talked about over the last two days is not an update of what Vincent says it's exactly the same in those those 11 years nothing changed.

01:01:11.440 --> 01:01:28.440
It's exactly the same story and that's what should make you very, very suspicious because genetic technologies have quadrupled or even by a couple orders of magnitude become more efficient and become more specific and become more powerful according to all these

01:01:28.440 --> 01:01:33.440
people that worked in the human genome project since they were in high school.

01:01:33.440 --> 01:01:53.440
And so if that's the case, then why hasn't our our resolution in virology increased why hasn't our our ability to measure these genetic signals in higher fidelity why don't we see that happening in virology why do we keep using the same things like plaque essays and this kind of thing

01:01:53.440 --> 01:01:55.440
why is that?

01:01:55.440 --> 01:02:00.440
And that huge question is never answered.

01:02:00.440 --> 01:02:06.440
It's never answered because there is no answer other than virology is a great big exaggeration.

01:02:06.440 --> 01:02:18.440
And it is covering up the fact that we understand that we have endogenous signaling that contributes at this regime and that this endogenous signaling is is part of our very biology.

01:02:19.440 --> 01:02:23.440
And so if they told us that then they wouldn't be able to study it at all.

01:02:23.440 --> 01:02:26.440
There would be no reason to mess around with it.

01:02:26.440 --> 01:02:31.440
Try to interfere with it to understand it.

01:02:31.440 --> 01:02:40.440
And there would be no excuse for them to, you know, sell you all kinds of cures for the the symptomologies of it. It's it's extraordinary.

01:02:40.440 --> 01:02:44.440
I'm sorry, I didn't mean to get off on a tangent there.

01:02:44.440 --> 01:02:48.440
Always good music.

01:02:48.440 --> 01:02:53.440
Professor Vincent rents in yellow. Oh, record yellow. It's actually not that bad.

01:02:53.440 --> 01:03:05.440
In the 1950s, the breakthrough in in virology was the demonstration that the nucleic acid of the virus is the genetic code.

01:03:06.440 --> 01:03:13.440
And how did they solve that problem? They solved that problem with a bacteriophage called T four.

01:03:13.440 --> 01:03:28.440
And it was the Hershey Chase experiment where I think they put some kind of radioactive radioactive marker and they could show that like the the phages took up the radioactive marker and that showed that the phages were being packaged with new DNA.

01:03:28.440 --> 01:03:30.440
I think that was something like that.

01:03:30.440 --> 01:03:36.440
And even though this sounds obvious today, it wasn't back then.

01:03:36.440 --> 01:03:43.440
People thought that the proteins, in fact, might be the important part because they were so much more complicated nucleic acids were simple.

01:03:43.440 --> 01:03:47.440
They only had four different chemicals in them.

01:03:47.440 --> 01:03:52.440
And this conclusion came from initially two kinds of studies.

01:03:53.440 --> 01:04:04.440
The Hershey Chase experiment, a very famous experiment which I'm sure you were taught in biology with bacteriophage T four shown here in an electron micrograph.

01:04:04.440 --> 01:04:11.440
And then the work of Frankel Conrad with tobacco mosaic virus.

01:04:11.440 --> 01:04:21.440
I'm not sure we're going to talk about this today, but what they did here was simply to take the virus and break it into RNA and protein components and showed that the RNA had the infectivity of the virus.

01:04:22.440 --> 01:04:25.440
Hershey Chase will talk about right here.

01:04:25.440 --> 01:04:28.440
So here is Al Hershey and Martha.

01:04:28.440 --> 01:04:34.440
So you could say it another way that if you expose cells to RNA, you can transfect them.

01:04:34.440 --> 01:04:41.440
And so infection or transfection is being distorted here.

01:04:41.440 --> 01:04:50.440
And so what if it's possible for my immune system to send out a signal that will transfect my children's immune system.

01:04:50.440 --> 01:04:58.440
In order to help them anticipate a signal that I've been exposed to.

01:04:58.440 --> 01:05:08.440
What if my immune system is sending out signals that are designed to help conspecific females decide whether I'm an attractive healthy male or not.

01:05:08.440 --> 01:05:15.440
Or whether I'm ill or have a genetic polymorphism that isn't compatible with one of theirs.

01:05:16.440 --> 01:05:28.440
We don't know any of these questions, but we do know that this signaling regime is not exclusively dominated by pathogens that are obligate parasites that are essentially molecules and fat bubbles.

01:05:28.440 --> 01:05:32.440
It can't be possible.

01:05:33.440 --> 01:05:49.440
Even bacteriophages are more accurately characterized as part of the natural biology of bacteria that you should think of them more as bacteria signaling to other bacteria rather than individually acting little organisms on their own.

01:05:49.440 --> 01:05:57.440
And I think that's the parallel mythological cartoon that they use with respect to bacteriophages.

01:05:57.440 --> 01:06:04.440
And it still wants you to think that that moonlander is separate from the bacteria and it's obviously it's very different and weird.

01:06:04.440 --> 01:06:09.440
So it must be different and separate.

01:06:09.440 --> 01:06:25.440
But that could be more akin to the two types of parts of a complicated plant life cycle, for example, rather than thinking of them as phages as as obligate parasites.

01:06:25.440 --> 01:06:31.440
Which in the context of a bacteria I think more clearly is kind of silly.

01:06:31.440 --> 01:06:40.440
Right if you think about these as obligate pathogens of bacteria it seems like well that's a pretty big statement mate doesn't it.

01:06:40.440 --> 01:06:46.440
It probably helps the bacteria as often as it hurts them like what's really going on here.

01:06:47.440 --> 01:06:52.440
It's how bacteria evolve more likely.

01:06:52.440 --> 01:07:02.440
And this is part of their biology but it's distorted in the exact same parallel way that exosomal signaling is distorted in mammals.

01:07:03.440 --> 01:07:11.440
And can exosomal signaling become aberrant and then be something like measles or polio it's possible and I definitely would not rule that out.

01:07:11.440 --> 01:07:13.440
I think it's very possible.

01:07:13.440 --> 01:07:31.440
But that's very different than suggesting that the exclusive content of this fraction in a sucrose gradient is viruses when we know that a very large portion of that fraction is almost guaranteed to be endogenous signaling in the same sort of chemical

01:07:31.440 --> 01:07:38.440
and and and physical size regime.

01:07:38.440 --> 01:07:57.440
And I think that virology can be thought of as that kind of mythology where they have just taken sacred biology that they understand is being much more complicated and intertwined with our own and told us a story about how is exclusively separate from us so that they can have a monopoly on understanding it.

01:07:57.440 --> 01:08:03.440
And weaponizing that monopoly against us and our grandchildren.

01:08:03.440 --> 01:08:15.440
Case they worked at Cold Spring Harbor for many years out on Long Island and they did this very famous experiment using a food blender common kitchen blender.

01:08:15.440 --> 01:08:26.440
And what they did they wanted to know if you take a bacteria page which is the genetic information which specifies the production of more viruses is that the protein shallower is at the nucleic acid.

01:08:26.440 --> 01:08:35.440
So they would grow phages with radioactive precursors to protein or radioactive precursors to the DNA.

01:08:35.440 --> 01:08:46.440
And when they labeled the DNA and then they would infect E. coli very briefly and then use a blender to shear off the infecting phase that was what the blender was for.

01:08:46.440 --> 01:08:53.440
So they only let the phage attach very briefly enough to put the genetic information in the cell.

01:08:53.440 --> 01:09:00.440
Because I mean it's you know obviously you can use a blender to remove phages right I mean that makes sense.

01:09:00.440 --> 01:09:08.440
And when they did this the radioactivity was predominantly found in the cell after a brief infection when you labeled the DNA.

01:09:08.440 --> 01:09:20.440
And then the DNA was detected in the next generation of phage and when you labeled the protein the coat of the virus that did not remain cell associated and it was never passed on to the next generation.

01:09:20.440 --> 01:09:26.440
So this is called the Hershey Chase Experiment. If you ever go out to Cold Spring Harbor for a meeting there's a library out there.

01:09:26.440 --> 01:09:34.440
I think it's called the Carnegie Library and they have one of Hershey's blenders in a glass case there for you to look at.

01:09:34.440 --> 01:09:37.440
It was out there this summer.

01:09:37.440 --> 01:09:44.440
So this was very important because it showed that the nucleic acid was the genetic information of the virus.

01:09:44.440 --> 01:09:53.440
Now even the bigger surprise I'm going to push the space bar perhaps above that the nucleic acid is the genetic information because in fact for cells we already knew that the DNA of cells was the genetic information.

01:09:53.440 --> 01:09:56.440
So this was a good move forward but not a big surprise but what really is surprising.

01:09:56.440 --> 01:10:01.440
I told you a lot about all the viruses that are out there billions and billions and all different kinds, shapes, forms.

01:10:01.440 --> 01:10:11.440
Yet in the end you can make them all fall into very nice categories because they only have a finite number of nucleic acid genomes and that is number seven.

01:10:11.440 --> 01:10:14.440
And that's a number you should remember.

01:10:14.440 --> 01:10:20.440
Yesterday I said six so I guess I skipped one. Sorry about that. I'm not a virologist. I just play one on Twitch.

01:10:20.440 --> 01:10:29.440
It's a subway number but it's going to help you remember all the different viral genomes because when you count up to seven you'll be done.

01:10:29.440 --> 01:10:35.440
Seven different kinds of genome and that will really help us organize all these viruses in terms of how they work.

01:10:35.440 --> 01:10:44.440
It will really help you. Maybe there are DNA retroviruses and RNA retroviruses and maybe I skipped the DNA retrovirus. That might be the problem.

01:10:44.440 --> 01:11:01.440
So the key fact that will really in addition to the number seven to help organize all of this is that every viral genome has to make mRNA that the host can translate because remember no virus encodes a complete translation apparatus,

01:11:01.440 --> 01:11:15.440
which is a parasites of the host translation system. So every virus. So they are parasites of the host translation system. That's a pretty interesting way to to phrase it.

01:11:15.440 --> 01:11:24.440
Whole viral genome viral genomes need to make it to mRNA and so they have lots of different ways of getting there, which we don't use.

01:11:24.440 --> 01:11:43.440
We don't copy our RNA apparently. We just copy our DNA and then we make RNA from our DNA and then we make proteins from our RNA using ribosomes but viruses are able to use double stranded DNA single stranded DNA plus and minus single stranded RNA and double stranded RNA.

01:11:43.440 --> 01:12:01.440
They're able to copy all of those molecules. They're able to, I mean, it's incredible. The kind of genetic diversity that viruses have developed in the context of being obligate parasites to our own cells.

01:12:01.440 --> 01:12:09.440
What a tremendous thing that is it's almost like a, yeah, it's almost like a story that doesn't make sense.

01:12:09.440 --> 01:12:18.440
Post this rule. And I would guess that if we ever found a virus that didn't, it probably wouldn't be a virus. It'd be a cell of some kind of very small cell.

01:12:18.440 --> 01:12:34.440
So all viruses have to make mRNA that can be read. So if you think of that, then every virus replication cycle is simple in the sense that it all has to lead to mRNA that can be translated by the host cell. And that's a ribosome. Not a turkey. This is a ribosome.

01:12:34.440 --> 01:12:43.440
It looks a lot like the other thing that DNA polymerase that it's not a turkey. It's a turducket.

01:12:43.440 --> 01:12:51.440
All right. Now, David Baltimore. There he is. David Baltimore with a big fish. That's awesome.

01:12:51.440 --> 01:12:55.440
Is that a red herring?

01:12:55.440 --> 01:13:14.440
Who was a Nobel laureate for discovering an enzyme that we'll talk about later on in another lecture. He used this insight, the idea that every virus has to make mRNA to organize all the known viral genomes into this scheme, which is nicely called the Baltimore scheme.

01:13:14.440 --> 01:13:26.440
And he put mRNA in the... The gapped DNA of the Hapatine virei day, Hapatine virei day viruses, the gapped DNA. Wow.

01:13:26.440 --> 01:13:39.440
So seven is not what I said it was. That's not RNA and retroviruses are RNA. And this gapped DNA, I don't know what that is.

01:13:39.440 --> 01:13:43.440
I'll have to go. I got so much to learn. It's just a great time to be alive.

01:13:43.440 --> 01:13:51.440
Middle. He said every genome has to lead to this. And then he arrayed around it all the known genomes. This was in the 70s that he did that.

01:13:51.440 --> 01:13:59.440
He put single-stranded DNA plus-sense RNA that goes through a DNA intermediate, other-plus-stranded RNA viruses.

01:13:59.440 --> 01:14:09.440
Dude, I have an oasterizer upstairs. It's a real oasterizer. And it actually says on it that it's a commercial one that we got at a house auction here when we first moved.

01:14:09.440 --> 01:14:15.440
It's like one of those old-school milkshake things. It has a square. But then the thing on the bottom of your mug has a star.

01:14:15.440 --> 01:14:23.440
And so it just goes on in every orientation. It's like back when they used to make stuff for real. And it has a sticker on it that says this is OSHA compliant.

01:14:23.440 --> 01:14:27.440
That shows you how old it is. Pretty cool. Oasterizer, baby.

01:14:27.440 --> 01:14:34.440
Negative-stranded RNA viruses. Double-stranded RNA viruses. We're going to talk about each of these in detail.

01:14:34.440 --> 01:14:41.440
He missed one. I didn't tell you double-stranded DNA, of course. He got that as well. All the ones that are numbered he got.

01:14:41.440 --> 01:14:49.440
But at the time, when he made this scheme, the Hepatitis B virus had not yet been discovered. Its genome wasn't known.

01:14:49.440 --> 01:14:53.440
And so that was added later. This funny double-stranded DNA with gaps.

01:14:53.440 --> 01:15:01.440
So he said, all of these have to go to mRNA. So we arrange them in this very nice rubric, which, if you learn, it will help you.

01:15:01.440 --> 01:15:07.440
So Hepatitis B has a double-stranded DNA with gaps as a genome. Interesting.

01:15:07.440 --> 01:15:14.440
Enormously, because given a viral genome, you'll be able to figure out how it's expressed. And we'll go through that today.

01:15:14.440 --> 01:15:20.440
David Baltimore, of course, likes the fish. That's him up there.

01:15:20.440 --> 01:15:27.440
So before we go on with the Baltimore scheme, let's do some definitions. So we're all talking the same language.

01:15:27.440 --> 01:15:33.440
And these have to do with polarity. As you know, probably, mRNA is, by convention, the plus strand.

01:15:33.440 --> 01:15:40.440
Someone asked me, last year, does it have to do with charge or something electricity? No, it has nothing to do with any of that.

01:15:40.440 --> 01:15:44.440
It's just convention that what is translated mRNA is called the plus strand.

01:15:44.440 --> 01:15:49.440
So a DNA strand that is the equivalent polarity as mRNA is the plus strand.

01:15:49.440 --> 01:15:55.440
And, of course, the complements of plus strands are negative strands or minus strands. So we use the word interchangeably,

01:15:55.440 --> 01:16:01.440
plus positive, minus negative. And it's just a way to identify what strand we're talking about.

01:16:01.440 --> 01:16:08.440
And, of course, mRNA, the plus strand is ribosome ready. It can be translated into protein. That's the definition of mRNA.

01:16:08.440 --> 01:16:14.440
But one thing I want to tell you is that not all plus RNA, especially in the virus world, is mRNA.

01:16:14.440 --> 01:16:20.440
There are some viruses that have plus-stranded RNA genomes, but it's not mRNA. It's not translated.

01:16:20.440 --> 01:16:26.440
So just being plus-stranded is not enough to get you translated. There are other requirements as well.

01:16:26.440 --> 01:16:37.440
So then when we look at the Baltimore scheme, these pluses and minus, that's what they refer to, the polarity of the nucleic acid with respect to mRNA.

01:16:37.440 --> 01:16:46.440
Now, just keep in mind that, I wonder if I have it in this deck. I don't have it in this deck. I would have to open up a different deck.

01:16:46.440 --> 01:17:00.440
And the standard cartoon for a coronavirus is that it's genome is translated, right? At least open reading frame 1A has to be translated.

01:17:00.440 --> 01:17:10.440
And then that poly protein needs to be cut up and formed into something that does a lot of other stuff. But

01:17:10.440 --> 01:17:28.440
plus-stranded RNA is not mRNA is an interesting statement. So we would need to look into it a little more into details about how well they have characterized the, let's say, translation of the coronavirus genome into the respective poly protein

01:17:28.440 --> 01:17:42.440
that then assembles into the RNA dependent RNA polymerase in the transcriptile, transcriptase complex thing that does all the heavy lifting of making new genomes.

01:17:42.440 --> 01:17:52.440
And in a coronavirus, that's a very special task. So because it's such a large genome and because it doesn't bring any proteins with it.

01:17:52.440 --> 01:18:08.440
The poly virus is supposed to have a sort of fragmented genome, which allows it to evolve and reassort a little faster, but it's also supposed to be packaged with its RNA dependent RNA polymerase because it's a negative stranded RNA.

01:18:08.440 --> 01:18:21.440
It has to have that in order for it to be able to turn its genomic negative strand into a positive strand, which can then be, can hijack ourselves.

01:18:21.440 --> 01:18:31.440
Those kinds of little subtleties are important to understand and important to have in your back pocket because a lot of these virologists don't even know these subtle differences off the top of their head.

01:18:31.440 --> 01:18:35.440
And it shows you how much they take for granted what they've memorized.

01:18:35.440 --> 01:18:46.440
For example, the single stranded plus strand DNA viruses. They have a single strand of DNA. It's plus polarity. That is the same polarity as mRNA.

01:18:46.440 --> 01:19:00.440
But of course, DNA cannot be translated. And that's one of the things you realize already by looking at the kind of nucleic acids that's present, you can already deduce what viruses have to do to get to mRNA.

01:19:00.440 --> 01:19:14.440
So this is really an elegant system. All you have to know is what kind of genome is in the virus, and then you can tell me all of the steps that have to be taken to get mRNA.

01:19:15.440 --> 01:19:18.440
Because in fact, when a virus, why is the genome into the cell?

01:19:18.440 --> 01:19:37.440
Why would it be that if you look at this scheme that we can see my arrow, why would it be that a virus with a single strand of DNA couldn't use this DNA to generate either a positive, no, it can't do positive, it would be negative.

01:19:37.440 --> 01:19:52.440
Why couldn't this positive stranded DNA use an enzyme to go first to negative RNA, and then that negative RNA could be translated to positive RNA? Why wouldn't it do it that way?

01:19:52.440 --> 01:20:01.440
Why is it obvious that it would go from a single stranded positive DNA molecule to double stranded DNA to mRNA?

01:20:02.440 --> 01:20:10.440
I don't think it's obvious that that happens here, and I think that's interesting. Let's just say part of this model.

01:20:10.440 --> 01:20:23.440
That's what it has to do. It has to make mRNA to initiate the infectious cycle. So if you can remember this scheme, you'll be able to trace it, and that's really a lot of what we want you to know in this course.

01:20:24.440 --> 01:20:33.440
So you don't have to memorize that there are billions and billions of viruses, but just that there are seven different genome types in the way that they get to mRNA.

01:20:33.440 --> 01:20:40.440
So here are the seven classes of genome. We have double stranded DNA, so there's a bunch of viruses with DNA genomes.

01:20:40.440 --> 01:20:48.440
They can be double stranded or single stranded, and they can be gapped. So that really covers everything.

01:20:48.440 --> 01:20:54.440
I can't think of anything else, single double stranded in a gap. The gap was thrown in later, but it makes sense.

01:20:54.440 --> 01:21:06.440
And then we have RNA viruses with RNA genomes, and this is, of course, what is in the particle itself? The virus particle, that's what I mean by the genome shown up here on this slide.

01:21:06.440 --> 01:21:16.440
So we have double stranded RNA genomes, and then we have single stranded RNA genomes, but we have single stranded RNA genomes of three types.

01:21:16.440 --> 01:21:26.440
So we have plus stranded. We have minus stranded, and then we have a plus stranded genome, which goes through a DNA intermediate.

01:21:26.440 --> 01:21:30.440
These are very special viruses that we'll deal with separately. So those are the seven classes.

01:21:30.440 --> 01:21:32.440
Those are the retroviruses down here.

01:21:32.440 --> 01:21:41.440
You may ask, why aren't there plus single stranded DNA viruses and minus single stranded DNA viruses? I don't know.

01:21:41.440 --> 01:21:54.440
There aren't any plus and minus DNA. There are some of the single stranded DNA viruses that package either a plus or a minus strand, but we don't know any that is exclusively either plus or minus like these RNA viruses.

01:21:54.440 --> 01:21:57.440
You just have to accept that they exist, and we don't know why.

01:21:57.440 --> 01:22:09.440
Now, in addition to these seven genome types, that's really the main thing you need to know, but I just want to show you the different kinds of structures that these seven types occur in.

01:22:09.440 --> 01:22:16.440
So they can be linear. They can be circular. They can occur in segments.

01:22:16.440 --> 01:22:23.440
So some viral genomes are in one molecule, unimolecular, if you will, and some are segmented. They come in pieces.

01:22:23.440 --> 01:22:28.440
You could say that our genome is segmented, right? It's in chromosomes. It's in pieces.

01:22:28.440 --> 01:22:34.440
And some viruses are segmented. They're not really chromosomes because they don't have the same structure, but they're some segmented.

01:22:34.440 --> 01:22:40.440
They're gapped, as we said already, with the hepatitis B. Single strands of plus and minus polarity.

01:22:40.440 --> 01:22:48.440
There's also a single stranded genome that's ambisense. It's got components of both plus and minus strands in it. Pretty unusual.

01:22:48.440 --> 01:22:54.440
We have double stranded, of course. And then some genomes have proteins, covalently attached to them.

01:22:54.440 --> 01:22:58.440
Some have the ends cross-linked. So you have a double-stranded DNA.

01:22:58.440 --> 01:23:00.440
I'm pushing this base bar.

01:23:00.440 --> 01:23:02.440
Five and three prime ends. They're just cross-linked. So you have a little circle at the end.

01:23:02.440 --> 01:23:04.440
So if in nature it would become a single stranded circle.

01:23:04.440 --> 01:23:07.440
And some have DNA with covalently attached RNA. So all kinds of things happen.

01:23:07.440 --> 01:23:10.440
Now, this is less important. These are the details, which we'll explain to you.

01:23:10.440 --> 01:23:12.440
But the seven is really important part. The seven are different genome types.

01:23:12.440 --> 01:23:14.440
Now, you may be wondering, what is the purpose of all this?

01:23:14.440 --> 01:23:20.440
Does having seven different genome types and all these weird configurations make a difference?

01:23:20.440 --> 01:23:27.440
Does it have to do with the biology of the virus? Does it have to do with evolution?

01:23:27.440 --> 01:23:32.440
Is it advantageous? And maybe most of all, you want to know if you have to memorize it.

01:23:32.440 --> 01:23:35.440
So let me try and answer. There's a lot of ignorance here, unfortunately. I can't answer most of these questions.

01:23:35.440 --> 01:23:40.440
But I sense that people think of this right away. Humans have the energy norms. All humans. All mammals do.

01:23:40.440 --> 01:23:45.440
So why do viruses have to have all these configurations? Well, we don't know the answer. All we can do is guess.

01:23:45.440 --> 01:23:47.440
And we get better at guessing.

01:23:47.440 --> 01:23:55.440
The reason why they have all these configurations is because they are reflections of our own complexity.

01:23:55.440 --> 01:24:03.440
It is very, very likely that many or all of these mechanisms are present in our own biology in some form or another.

01:24:03.440 --> 01:24:11.440
And are being wholly ignored as such. So that this entire field can exist in its current state.

01:24:12.440 --> 01:24:26.440
Which essentially a dual use scia that makes us think that all of the biology that is us is very, very simple and that all of the scary stuff out in the world we need protecting from.

01:24:26.440 --> 01:24:32.440
And this narrative has to be broken. We have to break it in our children. They have to be able to see this for what it is.

01:24:32.440 --> 01:24:49.440
It is a professor at Columbia University, one of the worst twisted weirdo places in America perpetuating a biology that he says hasn't essentially hasn't changed since he's been a student of David Baltimore.

01:24:49.440 --> 01:24:53.440
It's just absurd. It's absurd.

01:24:54.440 --> 01:25:04.440
So for example, the structure and composition of the genome, whether it's DNA, RNA, single or double stranded, is a reflection of how it replicates.

01:25:04.440 --> 01:25:11.440
But that's really because the replication has to accommodate the strandedness and the polarity and the composition of the genome.

01:25:11.440 --> 01:25:17.440
So it doesn't really tell us anything. There are viruses with all of these seven types of genomes out there.

01:25:17.440 --> 01:25:21.440
And I don't know if anyone has a selective advantage over another.

01:25:21.440 --> 01:25:29.440
For example, I would say, so if I'm looking at viruses from a human point of view, which is wrong, right, so you shouldn't do this as I told you before,

01:25:29.440 --> 01:25:34.440
I would say the single stranded RNA viruses would be the most advantageous.

01:25:34.440 --> 01:25:39.440
Because it's a plus strand. It's an mRNA. As soon as it gets in the cell, boom, it can be translated.

01:25:39.440 --> 01:25:42.440
I would say that this would be the fastest in the most advantageous.

01:25:42.440 --> 01:25:47.440
Now, there are a lot of plus strand viruses on the globe, but they're not the only ones.

01:25:47.440 --> 01:25:49.440
There are lots of other.

01:25:49.440 --> 01:25:56.440
I would think that self replicating RNA would actually be the most likely endogenous signal.

01:25:56.440 --> 01:26:14.440
If you wanted to transiently change the target of a biological signal, you would use a self replicating RNA in an exosome and put on the outside a receptor that was specific for the target like a T cell receptor or a B cell receptor.

01:26:14.440 --> 01:26:20.440
And then when that T cell or B cell took in that exosome, the RNA would be released.

01:26:20.440 --> 01:26:27.440
It would replicate itself for a little while, fill the ribosomes of that cell with that RNA signal.

01:26:27.440 --> 01:26:40.440
And for a very brief period of time, maybe that cell would change its physiology, change its signaling regime, go dormant, maybe even apoptose.

01:26:40.440 --> 01:26:53.440
But the point would be that this is gotta be, this cannot be ignored as something that obviously must come from us, otherwise they couldn't have it.

01:26:53.440 --> 01:27:06.440
We heard David Baltimore say yesterday that viruses couldn't evolve and tell cells evolve because they're obligate parasites that hijack our machinery and use it to make themselves.

01:27:06.440 --> 01:27:21.440
So if you hijack a cuckoo clock, can you make toast? No, you cannot. You could make gears. You could make a different arrangement of gears. You could make a fake cuckoo bird with a gear around its neck.

01:27:21.440 --> 01:27:28.440
You could make it ring at different times or ring different bells at different times of the day.

01:27:28.440 --> 01:27:33.440
You could probably make the hands go backwards.

01:27:33.440 --> 01:27:42.440
But because of the nature of a clock, you are limited what you can do with its parts and how you could use it in a different way, how you could hijack it.

01:27:42.440 --> 01:27:58.440
And even though a cell is much more complicated than a cuckoo clock, I would argue, and I think a legitimate, any legitimate biologist really thinking about this and realizing that exosomes are real, that people have been investigating

01:27:58.440 --> 01:28:13.440
exosomes in the context of cancer for a long time and that people like Bob Gallo and David Baltimore have sort of oversimplified it to say that these viruses are causing cancer rather than being a signaling regime that has sort of become

01:28:13.440 --> 01:28:29.440
dysregulated in the context of cancer, and therefore a very useful indicator of cancer and also likely an indicator that this signaling regime is actually used in health and well-being.

01:28:29.440 --> 01:28:36.440
That likely plays a very big role in health and homeostasis.

01:28:36.440 --> 01:28:53.440
And is probably likely to play a very central role in the regulation of the immune system that heretofore is understood to only use chemokines and cytokines to communicate between these very separate acting autonomous amoeba-like cells.

01:28:53.440 --> 01:29:10.440
Ignoring the possibility that retroviral signaling between these cellular regimes could cause a coordinated response, a coordinated dormancy, a coordinated crescendo and decrescendo of a highly specific inflammatory response.

01:29:10.440 --> 01:29:22.440
That all of the mechanisms by which this would be done are somehow or another exclusively the operating procedures of viruses.

01:29:22.440 --> 01:29:27.440
I just, I don't believe it anymore. I don't believe it at all anymore.

01:29:27.440 --> 01:29:28.440
Strand viruses.

01:29:28.440 --> 01:29:30.440
You should have told me to turn it up.

01:29:30.440 --> 01:29:35.440
As you'll see with a negative strain, you have to carry an enzyme in the particle in order to initiate infection.

01:29:35.440 --> 01:29:39.440
So, you see, none of this makes sense when you look at it from our point of view.

01:29:39.440 --> 01:29:48.440
But from an evolutionary point of view, at some time, a plus strand, a minus strand, a gapped genome, it worked, it survives.

01:29:48.440 --> 01:29:50.440
And that's all we can say at this point.

01:29:50.440 --> 01:29:53.440
Not a great answer, but that's what we're stuck with.

01:29:54.440 --> 01:29:55.440
Now, another...

01:29:55.440 --> 01:30:00.440
Despite all the progress we've made with the high-resolution genetic technologies, we're just stuck with it.

01:30:00.440 --> 01:30:05.440
The interesting question is, why do we have both DNA and RNA genomes in viruses?

01:30:05.440 --> 01:30:11.440
And that, I think, we can address a little better, although it's still speculative.

01:30:11.440 --> 01:30:21.440
I think you know the reason why is because we use exosomes with DNA and RNA in them to signal internally and externally.

01:30:22.440 --> 01:30:23.440
That's why.

01:30:23.440 --> 01:30:31.440
It doesn't need to have any other fancy explanation because David Baltimore told us that viruses couldn't evolve until cells had evolved.

01:30:31.440 --> 01:30:43.440
Viruses can't exist without the machinery of cells, without cells already doing something extremely similar to what they call viral infection,

01:30:43.440 --> 01:30:47.440
which is make exosomal signals and release them.

01:30:48.440 --> 01:30:51.440
Come on, ladies and gentlemen, we're there now.

01:30:51.440 --> 01:30:58.440
That's also why exosomes virtually disappeared from all the no-virus people's discussion.

01:30:58.440 --> 01:31:07.440
What I'd like you to do is go back and watch Mark Hewson-Tonic, Mark Koolack interview the man by the name of Mark Bailey.

01:31:07.440 --> 01:31:10.440
And you'll find in that interview at some point he asks him about exosomes.

01:31:10.440 --> 01:31:14.440
And does Mark Bailey say anything about exosomes that matters at all?

01:31:15.440 --> 01:31:18.440
No, he didn't know much about exosomes.

01:31:25.440 --> 01:31:39.440
And the other guy that I reminded you before who went back to this story about RNA genomes being dominant on the planet at some point in primordial earth was not other than Joe Lee,

01:31:40.440 --> 01:31:53.440
who now thinks that I guess I'm a controlled opera and idiot because I don't think it's all antibodies that are causing the vaccine injuries and chains of antibodies aren't the most important thing that they are for him.

01:31:53.440 --> 01:31:57.440
And so now he lost his mind.

01:31:57.440 --> 01:32:04.440
I see it's so clear now. It's just hilarious. I mean, there just aren't very, very, very many good guys. That's all.

01:32:04.440 --> 01:32:08.440
There are lots and lots of meddlers, lots and lots of players.

01:32:09.440 --> 01:32:13.440
Witting and unwitting players, but they're all players now.

01:32:13.440 --> 01:32:25.440
Because if you're not fighting for the people that they've murdered and lied about, if you're not fighting for the deaths that they lied about and how those lies were used to bully people into accepting

01:32:26.440 --> 01:32:33.440
transfection. Instead, you're complaining about the transfection or its adulteration or its rush or who paid for it or who got profit from it.

01:32:33.440 --> 01:32:44.440
Then you're not fighting against this mythology, which is the foundation of the vaccine schedule in America, which compared to any other vaccine schedule is obviously criminal, even to an eight year old.

01:32:47.440 --> 01:32:48.440
And they won't talk about it.

01:32:49.440 --> 01:32:58.440
They won't talk about the murder and they won't talk about the vaccine schedule. They just want to talk about the transfection as a vaccine and then it wasn't perfect.

01:33:00.440 --> 01:33:03.440
And they want to talk about lab leak and how they lied about it.

01:33:04.440 --> 01:33:14.440
You know that many people believe that the first world of life was an RNA world where all the organisms had RNA genomes.

01:33:14.440 --> 01:33:19.440
There's some evidence, some pretty good evidence for this from a variety of areas.

01:33:19.440 --> 01:33:24.440
So we think that these RNA viruses evolved during the RNA world.

01:33:24.440 --> 01:33:29.440
So there are RNA cells first and then RNA viruses evolved probably from those cells.

01:33:30.440 --> 01:33:37.440
I mean, do we even know that there was an RNA world before there was a DNA world? I mean, that's amazing. I mean, that is really impressive.

01:33:37.440 --> 01:33:43.440
Oh, no, to infect them. And those RNA viruses that existed then are probably...

01:33:43.440 --> 01:33:51.440
It's a lot more complicated explanation than saying that, well, we signal using similar things that we would call exosomes.

01:33:51.440 --> 01:33:59.440
And occasionally, I guess it's possible that exosomal signaling can go wrong and you can have something like measles or polio.

01:33:59.440 --> 01:34:08.440
That would be a very interesting sort of explanation for pathogenesis and pathogenic diseases in the context of our own biology.

01:34:08.440 --> 01:34:18.440
But instead, they make up this crazy story that has to do with accepting a theory of primordial Earth life generation.

01:34:18.440 --> 01:34:22.440
We actually have to accept how life was spontaneously generated.

01:34:22.440 --> 01:34:29.440
If you want to believe that viruses exist in the context of Vincent Rancin yellow's biology, do you understand that?

01:34:29.440 --> 01:34:42.440
His class has to accept in this explanation that life was spontaneously generated on Earth by lightning hitting a mud puddle with nucleic acids in it.

01:34:43.440 --> 01:34:56.440
And that's how he explains the existence of viruses, even though his teacher David Baltimore told us in 2018 from the video two days ago that viruses couldn't evolve until cells existed.

01:34:56.440 --> 01:35:05.440
Which is a lot closer to the truth than I think he ever intended to be, which is that viruses, if they are anything, are just us.

01:35:06.440 --> 01:35:15.440
They are just our signaling or our signaling gone wrong or our signaling functioning in a way that is being mischaracterized as a pathogen.

01:35:15.440 --> 01:35:24.440
It could also be our interaction with these signals that have their own homolog and other animals and insects and plants and other night.

01:35:24.440 --> 01:35:33.440
And it could also be, of course, the huge background of the same signal homolog in bacteria, and that is bacteriophages.

01:35:36.440 --> 01:35:51.440
And multicellular organisms like mammals like ourselves have grown and evolved and existed in the context of this amazingly complex background for what we say are thousands of years.

01:35:51.440 --> 01:36:03.440
So if that's the case, then this story of having to accept how this all came to be by lightning in a mud puddle is also a very interesting posit to put into this basic virology lecture.

01:36:03.440 --> 01:36:06.440
Extraordinary, in fact.

01:36:06.440 --> 01:36:13.440
The ancestors of all the RNA viruses that we have today, so maybe our RNA viruses are relics from that RNA world.

01:36:14.440 --> 01:36:20.440
Now, at some point, the idea goes that there's a switch from RNA to DNA-based organisms.

01:36:20.440 --> 01:36:26.440
Or maybe not a switch, but DNA-based organisms evolved, and the whole idea behind this is very interesting.

01:36:26.440 --> 01:36:35.440
But there was another kind of organism that had DNA, and these RNA-based organisms slowly were competed out, but the RNA viruses lived.

01:36:35.440 --> 01:36:39.440
Why would there be a switch? We have no idea. We don't know what the selection would be for that.

01:36:39.440 --> 01:36:49.440
It could be that a DNA-based organism arose, so enzymes arose that could change RNA chemically into DNA, and they survived, and they had some kind of advantage.

01:36:49.440 --> 01:37:02.440
That's really all we can say. So the way I look at it is there are motorcycles, and then at some point in time, some screwdrivers showed up, and then people started to take the motorcycles apart and put them back together, and then cars came.

01:37:03.440 --> 01:37:08.440
And then we got some more wrenches. They flew. They just appeared. I don't know how they really came about.

01:37:08.440 --> 01:37:11.440
And now we have mechanics and cars. That's how it works.

01:37:11.440 --> 01:37:14.440
RNA viruses are pretty old. They've been very successful.

01:37:14.440 --> 01:37:19.440
I smell fire. What does this smell? The DNA viruses are the newcomers on the block.

01:37:19.440 --> 01:37:23.440
Now, in terms of memorization, this is the one thing I think we should memorize.

01:37:23.440 --> 01:37:30.440
The number seven is easy. You have lots of number seven references here in New York City.

01:37:30.440 --> 01:37:39.440
And this scheme, which is very easy to do, you put mRNA in the middle, and then draw the seven different kinds of viral genome around it.

01:37:39.440 --> 01:37:42.440
I don't care if you know the numbers here. That doesn't mean anything.

01:37:42.440 --> 01:37:48.440
All you need to do is draw all these viral genome types, and then be able to draw the pathway to mRNA.

01:37:49.440 --> 01:37:53.440
Because just knowing that, you're going to conquer the first half of this course.

01:37:53.440 --> 01:37:58.440
Because you're going to understand what we're talking about when we talk about RNA and DNA synthesis and so forth.

01:37:58.440 --> 01:38:03.440
Now, as we illustrate these processes, we'll use specific viruses as examples, and you see I put a few of them here.

01:38:03.440 --> 01:38:05.440
So you know what virus has a single-stranded negative strand genome.

01:38:05.440 --> 01:38:07.440
Otherwise, it would be really not interesting to you at all.

01:38:07.440 --> 01:38:09.440
So negative strand RNA viruses include influenza and Ebola, polio and plus-strand.

01:38:09.440 --> 01:38:11.440
Retroviruses are the ones that go from a plus-strand to a DNA intermediate.

01:38:11.440 --> 01:38:14.440
Carval viruses have to tie to B, adenovirus, herpes-reo, and rotavirus.

01:38:14.440 --> 01:38:17.440
These are just a few of all the viruses that exist.

01:38:17.440 --> 01:38:21.440
But we're going to come back to these over and over and use them as examples.

01:38:21.440 --> 01:38:25.440
So you should know that rheovirus has a double-stranded RNA genome.

01:38:25.440 --> 01:38:34.440
So you guys wonder, why would it be that coronaviruses, for some reason or another, don't need to go this way?

01:38:37.440 --> 01:38:39.440
Coronaviruses don't do that, right?

01:38:39.440 --> 01:38:45.440
So they're just a positive-stranded RNA molecule that gets translated right away to mRNA as far...

01:38:45.440 --> 01:38:47.440
I mean, gets translated right away to protein.

01:38:49.440 --> 01:38:50.440
Did you know that?

01:38:51.440 --> 01:38:53.440
They are essentially a transfection.

01:38:53.440 --> 01:38:58.440
They are just a lipid nanoparticle with RNA inside of them.

01:38:59.440 --> 01:39:02.440
They're not even really on here, which is really weird.

01:39:03.440 --> 01:39:12.440
And if this is where coronaviruses belong, then we never really talk about this negative-stranded RNA very much.

01:39:15.440 --> 01:39:18.440
Is that the template from which the genome is made?

01:39:18.440 --> 01:39:20.440
Is that the first thing that gets made?

01:39:20.440 --> 01:39:23.440
It can't be because they don't come with proteins, you see?

01:39:24.440 --> 01:39:26.440
Very curious how that fits.

01:39:26.440 --> 01:39:27.440
I'm very curious.

01:39:28.440 --> 01:39:35.440
Can we say on a test, a real virus is what, when the genome enters the cell, you're going to have to know that it's double-stranded RNA.

01:39:35.440 --> 01:39:39.440
So this one slide is very important, but this is very easy to do.

01:39:40.440 --> 01:39:44.440
Okay, so memorize these seven genome types, you should start doing it.

01:39:44.440 --> 01:39:48.440
Maybe you could wait until the first exam, if you're good at that sort of thing.

01:39:48.440 --> 01:39:50.440
And this is what I want you to know.

01:39:50.440 --> 01:39:53.440
So memorize this stuff, so then let's go to the next one.

01:39:53.440 --> 01:39:57.440
Let's go to the next one because I want to get this done before 1230.

01:39:57.440 --> 01:39:59.440
Let's do this quick here.

01:39:59.440 --> 01:40:00.440
This is the next one.

01:40:01.440 --> 01:40:11.440
This is genetics of virology, and this one's really good because he's going to gloss over the infectious clone and how important it is, but gloss over it in a way that reveals it anyway.

01:40:12.440 --> 01:40:21.440
I want to finish up today by talking about how we, the modern way of manipulating virus genomes, how we do genetics and how we study them and how we use them.

01:40:21.440 --> 01:40:25.440
We can actually use viruses to treat diseases, but we're trying to anyway.

01:40:26.440 --> 01:40:35.440
And just to make sure we are on the same page in terms of definition, we use wild type a lot in virology, and you've heard this in other areas of biology as well.

01:40:35.440 --> 01:40:39.440
For viruses, this simply means that you're standard.

01:40:39.440 --> 01:40:46.440
Whatever is in your lab and is your standard virus, you call it wild type, and then you make your mutants from the wild type strain.

01:40:46.440 --> 01:40:51.440
It may not be the same as a virus that is infecting people or animals out there in nature.

01:40:51.440 --> 01:40:58.440
It may have been isolated years ago, and you've passed it in your laboratory, but it's your wild type, so that's all that means.

01:40:59.440 --> 01:41:12.440
When we get viruses from the host, a clinical specimen or an animal, we call these field isolates or clinical isolates, and those can be brought in the lab and they could become your wild type if you wish, but not unless it's a brand new virus.

01:41:12.440 --> 01:41:18.440
Not unless it's a brand new virus, so that's a little slip up right there already, I think, if we analyze it a little bit.

01:41:19.440 --> 01:41:29.440
So, a wild type virus is often laboratory adapted, so it grows in a dish, whereas all the ones that you get from the wild don't necessarily grow in a dish.

01:41:29.440 --> 01:41:32.440
That's kind of a caveat already.

01:41:32.440 --> 01:41:42.440
What he basically means is that almost none of them grow, and when they do grow, we grow them, but you're not going to be able to get another one from the wild and get it to grow.

01:41:43.440 --> 01:41:53.440
And if it does grow, it doesn't matter, because he has, he said, unless it's a whole new virus, you can't use that as a laboratory stock. Anyway, that's a little weird.

01:41:53.440 --> 01:42:00.440
That's already kind of admitting that stock is important, and that where you get your stock is important.

01:42:01.440 --> 01:42:24.440
And I think Mark Kulak has also identified an interesting historical stock story about some lady by the name of Alice Moore, who is curiously connected with a lot of different early culturing cell culture, cancer people, and has a house next to it within a mile or two of all of Henry Kissinger's houses.

01:42:24.440 --> 01:42:35.440
Anyway, that's Mark Kulak's stuff. I don't do people here, at least not very often, except for the people that have directly had contact with me and my family. Those people I'm happy to do shows about.

01:42:35.440 --> 01:42:46.440
So here we are again, we're trying to define some of these terms, and what we should really be saying is redefining these terms under the auspicious of virology, because that's what they've done.

01:42:47.440 --> 01:42:56.440
They've taken a lot of biological terms and redefined them under their own rubric with the idea of saying, well, in the context of virology, this is what we're talking about.

01:42:56.440 --> 01:43:10.440
Wild type means the laboratory strain that grows. Wild type doesn't mean that one out in the wild, like it does with every other field in virology.

01:43:10.440 --> 01:43:21.440
So let's start out with a giant contradiction or a giant inversion. I think a lot of the lies are essentially inversions because inversions are the ones that are easiest to confuse, right?

01:43:21.440 --> 01:43:31.440
Is this my left hand or my right hand? When one of them's true and one of them's false, this is the best lie, right?

01:43:31.440 --> 01:43:34.440
That's what you see here.

01:43:34.440 --> 01:43:40.440
And so in viral isolates from the natural host, our clinical isolates, I'll let him do it.

01:43:40.440 --> 01:43:48.440
Now an important method that we use in virology is DNA mediated transformation. This is when we put DNA into cells, we take cells in culture.

01:43:48.440 --> 01:44:02.440
Now this is an interesting history lesson that I think we can take pretty much seriously, the origin of transformation and transfections, the words, but the modern use has segregated because of the commercial products that have come behind them.

01:44:02.440 --> 01:44:15.440
And commercial products, I assure you, are segregated into transformation products, which are based on DNA and transfection products, which are usually exclusively based in RNA.

01:44:15.440 --> 01:44:33.440
Now it can be that throughout this early history of the development of these technologies, these two words were interchanged, mainly because, and keep this very clearly in mind, anybody who cut their teeth in the Human Genome Project during their high school science project

01:44:33.440 --> 01:44:40.440
could tell you that we weren't always able to make RNA as easily as we could make DNA.

01:44:40.440 --> 01:44:56.440
And so there was a time when transformation and transfection might have been kind of the same word used to describe basically the same thing, which is used DNA to express a protein in a place where it's normally not found.

01:44:56.440 --> 01:45:11.440
But as we started to commercially be able to produce RNA at a similar purity, it now became sort of commercially standard to call transformation any product to do with DNA and transfection to do with specifically RNA.

01:45:12.440 --> 01:45:27.440
As long as you understand that this is historically just probably preceding this explosion of commercial products that were taking advantage of the cheapness of making RNA and DNA, that you'll see this history as it is.

01:45:27.440 --> 01:45:42.440
And I think it's pretty good history, but it also accentuates the fact that any of these people could have called the investigational vaccines, either the adenovirus vaccines or the RNA lipid nanoparticle vaccines.

01:45:42.440 --> 01:45:54.440
They could have called them transformations and transfections as I've insisted they should have from the very beginning and come closer to giving people informed consent over what was coerced into their bodies.

01:45:54.440 --> 01:45:58.440
But they didn't. Brett Weinstein didn't.

01:45:58.440 --> 01:46:21.440
I mean, CHD hasn't like all of these organizations, all of these people that I've been talking to since 2020 and beyond a very, very, very, very tiny fraction, very close to zero, have consistently been able to say these are not vaccines, they are transfections.

01:46:21.440 --> 01:46:31.440
And we are the only people, giggle on biologicals, the only source of information you're going to find that's accurate enough to tell you that from day one.

01:46:31.440 --> 01:46:40.440
So let's listen to Vincent define these two words and expand our understanding about the sort of semantic war that has been waged on us.

01:46:40.440 --> 01:46:44.440
We take purified DNA, we add it to the cells and the cells take it up.

01:46:44.440 --> 01:46:48.440
There are tricks that we use to get the cells to take it up. It's not very hard.

01:46:48.440 --> 01:46:51.440
It's called DNA mediated transformation.

01:46:51.440 --> 01:46:55.440
So why do we say this long word to describe it?

01:46:55.440 --> 01:47:01.440
Well, transformation has to do with oncogenic transformation of cells, making tumors, for example.

01:47:01.440 --> 01:47:02.440
We'll talk about that later.

01:47:02.440 --> 01:47:07.440
So when people first described this process, they called it DNA mediated.

01:47:07.440 --> 01:47:11.440
Now you may say, why did they call it transformation at all?

01:47:11.440 --> 01:47:22.440
And that's a historical thing that goes back to 1944, this paper by Avery McCloud and McCarthy from right here in New York City at the Rockefeller Institute.

01:47:22.440 --> 01:47:31.440
They found you could transform pneumococci bacteria into either a rough or a smooth colony by putting DNA

01:47:31.440 --> 01:47:34.440
from one morphology into the other.

01:47:34.440 --> 01:47:38.440
So we're transforming the cells with DNA.

01:47:38.440 --> 01:47:41.440
This was actually the proof that DNA was genetic material.

01:47:41.440 --> 01:47:46.440
So Rockefeller Institute, that's kind of interesting, of course.

01:47:46.440 --> 01:47:47.440
It is after that.

01:47:47.440 --> 01:47:48.440
I didn't believe it as well.

01:47:48.440 --> 01:47:51.440
So transformation came from this paper.

01:47:51.440 --> 01:47:54.440
Transformation of pneumococcal types.

01:47:54.440 --> 01:47:56.440
So we're stuck with it.

01:47:56.440 --> 01:47:59.440
So we call it DNA mediated transformation.

01:47:59.440 --> 01:48:02.440
So he says we're stuck with it, but we're really not stuck with it.

01:48:02.440 --> 01:48:14.440
What we are, it's as evolved since commercial products to use DNA and RNA to express proteins have been developed and used as these technologies and methodologies have expanded.

01:48:14.440 --> 01:48:16.440
Confused with oncogenesis.

01:48:16.440 --> 01:48:28.440
Now when we take a virus genome and we put it into a cell and we get virus out, this is called transfection, the production of infectious virus after DNA mediated transformation with viral DNA.

01:48:28.440 --> 01:48:31.440
First shown with the lambda DNA.

01:48:31.440 --> 01:48:32.440
And transfection.

01:48:32.440 --> 01:48:34.440
First shown with a bacteriophage, right?

01:48:34.440 --> 01:48:36.440
Sorry, first shown with a bacteriophage.

01:48:36.440 --> 01:48:40.440
It comes from this word, transformation, infection.

01:48:40.440 --> 01:48:44.440
It was coined by the people who did the lambda studies.

01:48:44.440 --> 01:48:50.440
Now, unfortunately everybody uses transfection to just mean DNA mediated transformation.

01:48:50.440 --> 01:48:51.440
It's not quite right.

01:48:51.440 --> 01:48:53.440
So we try and use the right terminology.

01:48:54.440 --> 01:49:00.440
But if you pick up a catalog from a biological supplier, they'll list transfection reagents.

01:49:00.440 --> 01:49:01.440
There you go.

01:49:01.440 --> 01:49:17.440
And specifically, I would argue that over the years, maybe the years that that proceeded from this, that that segregation became more clear, especially in the commercial space transformation was DNA, transfection is RNA and that's where I came out in 2020 was like, dude.

01:49:18.440 --> 01:49:19.440
That's transformation.

01:49:19.440 --> 01:49:20.440
This is transfection.

01:49:20.440 --> 01:49:23.440
And neither of them are investigational vaccines.

01:49:23.440 --> 01:49:24.440
These people are lying.

01:49:24.440 --> 01:49:25.440
All right.

01:49:25.440 --> 01:49:29.440
Because it's easier to say transfection than DNA mediated transformation.

01:49:29.440 --> 01:49:36.440
But I just want you to know that transfection, in our view, means making viruses from DNA.

01:49:36.440 --> 01:49:44.440
Now, when you genetic methods involve altering the virus genome so that you can study the function of genes.

01:49:44.440 --> 01:49:45.440
Okay.

01:49:45.440 --> 01:49:46.440
We make changes in DNA.

01:49:46.440 --> 01:49:47.440
That's what mutation is.

01:49:47.440 --> 01:49:51.440
Changes in DNA or RNA can make nonsense or missense mutation.

01:49:51.440 --> 01:49:53.440
You know all this stuff.

01:49:53.440 --> 01:49:57.440
But the point I want to make here is that a mutation is not what you do to a protein.

01:49:57.440 --> 01:50:01.440
A mutation was originally coined to describe changes in DNA.

01:50:01.440 --> 01:50:05.440
So we make mutations in viral genomes, and then we study the phenotypes.

01:50:05.440 --> 01:50:07.440
The resulting proteins are altered.

01:50:07.440 --> 01:50:12.440
They have amino acid changes or insertions or deletions, but they're not mutated proteins.

01:50:12.440 --> 01:50:16.440
This mutation is a activity on nucleic acid.

01:50:16.440 --> 01:50:23.440
Now, this assay, which we talked about last time, the plaque assay, is what made possible genetic analysis of viruses.

01:50:23.440 --> 01:50:30.440
Because you can then start to isolate clonal populations of viruses by picking virus from individual plaques.

01:50:30.440 --> 01:50:34.440
Do those look like individual plaques to you?

01:50:34.440 --> 01:50:38.440
Here are some of these plaques are very close together.

01:50:38.440 --> 01:50:43.440
So you definitely want to do multiple plaque pickings as we said last time.

01:50:43.440 --> 01:50:46.440
So plaque assay allows genetic methods to go forward.

01:50:46.440 --> 01:50:55.440
So from 1952, when Dobekko made the plaque assay for animal viruses, from that point on, genetics of viruses could be done.

01:50:55.440 --> 01:51:00.440
So from that point on, we really haven't improved the way that we isolate viruses.

01:51:00.440 --> 01:51:02.440
We use plaque assays.

01:51:02.440 --> 01:51:07.440
And we believe that these things are working.

01:51:08.440 --> 01:51:12.440
And they probably do for certain signals like bacteriophages.

01:51:12.440 --> 01:51:15.440
I wonder if plaque assays work for coronavirus.

01:51:15.440 --> 01:51:21.440
I wonder if they work for exosomes.

01:51:21.440 --> 01:51:29.440
And I think that's where this whole mythology becomes a gross over exaggeration of understanding.

01:51:29.440 --> 01:51:31.440
And this is what you get.

01:51:31.440 --> 01:51:36.440
You get somebody who can tell you a very simple story.

01:51:36.440 --> 01:51:41.440
He'll tell you a very simple picture and not need to explain anything because he can't.

01:51:41.440 --> 01:51:47.440
Number four with bacteriophage because the plaque assay was developed for them earlier.

01:51:47.440 --> 01:51:56.440
Now in the old days, people would have their virus and look for mutants in a tube of virus that they had in the laboratory.

01:51:56.440 --> 01:52:02.440
They had no way of really directing mutations to specific parts of the genome.

01:52:02.440 --> 01:52:09.440
As I said earlier, and we will come back to this many times, RNA virus polymerases are highly error-prone.

01:52:09.440 --> 01:52:14.440
They make one missing corporation and every 10,000 to 100,000 nucleotides that they polymerize.

01:52:14.440 --> 01:52:16.440
And they don't correct these errors.

01:52:16.440 --> 01:52:21.440
So when you just grow up a stock of influenza virus, it's not one virus.

01:52:21.440 --> 01:52:22.440
It's a collection of mutants.

01:52:22.440 --> 01:52:27.440
And all you have to do is apply the right selection to get the mutant out you want.

01:52:27.440 --> 01:52:36.440
If you want a viral mutant resistant to an antibody or a drug, you just apply selection and you can easily get it from a stock of RNA viruses.

01:52:36.440 --> 01:52:40.440
DNA viruses have lower missing corporation rates.

01:52:40.440 --> 01:52:43.440
Mutations are harder to come by in their genomes.

01:52:43.440 --> 01:52:49.440
So typically you treat them with chemicals that mutagenize the DNA and then screen for your phenotypes.

01:52:49.440 --> 01:52:51.440
So these are some of the phenotypes you can screen for.

01:52:51.440 --> 01:52:53.440
As I said, drug resistance plaque size.

01:52:53.440 --> 01:52:57.440
If your virus makes a big plaque, you see a small plaque on the plate.

01:52:57.440 --> 01:53:00.440
It's a mutant. You pick it and study it and see what gene has been altered.

01:53:00.440 --> 01:53:03.440
All right, so this is the old style of genetics in viruses.

01:53:03.440 --> 01:53:06.440
We don't do this anymore because it's too haphazard.

01:53:06.440 --> 01:53:12.440
Now what we do is we have, for most viruses that are studied, what are called infectious DNA clones.

01:53:12.440 --> 01:53:17.440
You take the viral genome, whether it's DNA or RNA, you make a DNA copy of it.

01:53:17.440 --> 01:53:21.440
You amplify it in a bacterial plasmid so you can get lots of it.

01:53:21.440 --> 01:53:24.440
And then you take that DNA and you put it into a cell.

01:53:24.440 --> 01:53:28.440
You transfect the cell and the cell will make infectious virus.

01:53:28.440 --> 01:53:38.440
Wow, and did you hear how he stated that so very clearly you don't even need to use a T5 polymerase to make RNA from the DNA?

01:53:38.440 --> 01:53:41.440
You can just put the DNA on cells and they'll make the virus.

01:53:41.440 --> 01:53:42.440
Did you hear it?

01:53:42.440 --> 01:53:44.440
Because that's what he means.

01:53:45.440 --> 01:53:49.440
That's what I have kind of been suggesting for a while.

01:53:49.440 --> 01:53:58.440
And one of the things that I think they talked to me out of as part of the obfuscation interference when they were still like,

01:53:58.440 --> 01:54:01.440
Oh, infectious clones is kind of a good idea.

01:54:05.440 --> 01:54:13.440
I really think it's important to realize that once they told you that they make the DNA copy of whatever nonsense they say they found

01:54:13.440 --> 01:54:17.440
in the wild, everything after that is synthetic.

01:54:17.440 --> 01:54:25.440
Nothing about whatever happens when they take that DNA and put it somewhere else has anything to do with the original signal

01:54:25.440 --> 01:54:31.440
that they say they found in the wild, whether it's in a bat or a raccoon dog or in that sick child.

01:54:34.440 --> 01:54:41.440
Because that doesn't recapitulate whatever they say the signal they're finding because now they have a quantity

01:54:41.440 --> 01:54:44.440
and a purity that they didn't find in the first place.

01:54:44.440 --> 01:54:52.440
So how can they say that this purity level even matters that it even represents anything related to that shadow of a signal

01:54:52.440 --> 01:55:00.440
that they purport to have found in whatever clinical or patient sample or whatever they found?

01:55:03.440 --> 01:55:09.440
They make infectious DNA clones of these things and then they play with them.

01:55:09.440 --> 01:55:11.440
And that's how they can make a lot of it.

01:55:11.440 --> 01:55:17.440
That's how they could put all these signals in different places and make it look like that guy had a PCR positive test.

01:55:17.440 --> 01:55:19.440
That guy had a sequence in him.

01:55:19.440 --> 01:55:21.440
That guy's virus was cultural.

01:55:21.440 --> 01:55:29.440
All of those things can be created through the generation of a DNA infectious clone with the spike protein sequence

01:55:29.440 --> 01:55:37.440
all by itself would be enough to put the spike protein DNA and RNA in all these places to put this toxin in all these places

01:55:37.440 --> 01:55:40.440
to put this signal in all these places.

01:55:40.440 --> 01:55:42.440
But if you wanted the whole genome you could do that too.

01:55:42.440 --> 01:55:46.440
If you wanted the spike protein and the end protein you could put those to them.

01:55:48.440 --> 01:55:55.440
Any number of combinations are possible and you could generate commercial quantities of them

01:55:55.440 --> 01:55:59.440
because we have been making these things for years.

01:55:59.440 --> 01:56:03.440
We've been making large quantities of RNA DNA and protein for years.

01:56:07.440 --> 01:56:11.440
And so it doesn't need to be the magic viruses.

01:56:11.440 --> 01:56:13.440
It doesn't need to be.

01:56:15.440 --> 01:56:17.440
It can just be biotechnology.

01:56:17.440 --> 01:56:32.440
It can just be the silly, you know, very clumsy things that we can do with DNA and RNA and that's enough to explain all the crap that they call virology.

01:56:32.440 --> 01:56:34.440
That's what I think we're dealing with here.

01:56:34.440 --> 01:56:45.440
And if it actually is just them obfuscating the irreducible complexity of our own biology and that of the biology around us

01:56:45.440 --> 01:56:47.440
then we have a very, very big problem.

01:56:47.440 --> 01:56:52.440
If we're going to pass this mythology to our kids they are going to become enslaved by it.

01:56:52.440 --> 01:56:55.440
Bacterial plasmid so you can get lots of it.

01:56:55.440 --> 01:56:58.440
And then you take that DNA and you put it into a cell.

01:56:58.440 --> 01:57:02.440
You transfect the cell and the cell will make infectious virus.

01:57:02.440 --> 01:57:07.440
This is actually a modern validation of Al Hershey and Martha Chase's experiment, right?

01:57:07.440 --> 01:57:11.440
It shows that the DNA is the genetic material of the genome.

01:57:11.440 --> 01:57:14.440
And you can make all kinds of changes to DNA.

01:57:14.440 --> 01:57:22.440
So if you had an RNA virus like influenza virus the only thing you could ever do was to treat it with chemicals or just try and select out mutants from the population.

01:57:22.440 --> 01:57:25.440
But now you can introduce specific mutations.

01:57:25.440 --> 01:57:27.440
You can make recombinant viruses.

01:57:27.440 --> 01:57:29.440
You can make anything you want.

01:57:29.440 --> 01:57:31.440
And in fact people are very afraid of this.

01:57:31.440 --> 01:57:40.440
You only have to look online to see people who think that the genetic engineers are going wild with viruses and making viruses that are going to kill everyone.

01:57:40.440 --> 01:57:44.440
Well this is not entirely true of course but we can modify them.

01:57:44.440 --> 01:57:47.440
It's not entirely true of course.

01:57:47.440 --> 01:57:49.440
He actually said that.

01:57:49.440 --> 01:57:52.440
It's not entirely true of course.

01:57:52.440 --> 01:57:56.440
Ladies and gentlemen they've been planning this for a very long time.

01:57:56.440 --> 01:57:59.440
They've been seeding this narrative into the beginning of movies.

01:57:59.440 --> 01:58:01.440
The plots of movies.

01:58:01.440 --> 01:58:03.440
The plots of TV shows.

01:58:03.440 --> 01:58:04.440
Come on guys.

01:58:04.440 --> 01:58:10.440
It hasn't been in the Simpsons and the X-Files and the new Planet of the Apes and a million other places for nothing.

01:58:10.440 --> 01:58:24.440
It hasn't been in the sparse pandemic document and in the dark winter documents and the event 201 documents and in the lectures of James Giordano for the last 15 years for nothing ladies and gentlemen.

01:58:24.440 --> 01:58:25.440
Come on.

01:58:25.440 --> 01:58:26.440
That will.

01:58:26.440 --> 01:58:33.440
So for example with poliovirus the virus contains an RNA genome plus stranded.

01:58:33.440 --> 01:58:39.440
If you extract that RNA from the particle which you can do and you can purify the RNA.

01:58:39.440 --> 01:58:45.440
If you inserted that into cells by transfection that would initiate an infectious cycle.

01:58:45.440 --> 01:58:46.440
Just the mRNA.

01:58:46.440 --> 01:58:53.440
See RNA into this thing is called transfection and he says the viral DNA is called transfection.

01:58:54.440 --> 01:59:03.440
And my argument is that we became more precise in recent years and we have segregated transfection to RNA and transformation to DNA.

01:59:03.440 --> 01:59:14.440
And that was the argument that I was making in 2020 when I got asked to not come into the University of Pittsburgh School of Medicine anymore because they thought I was a danger.

01:59:15.440 --> 01:59:18.440
Because it just has to be translated, make proteins and you initiate replication.

01:59:18.440 --> 01:59:22.440
So the RNA is infectious but we can't really modify RNA.

01:59:22.440 --> 01:59:31.440
So what we did was to make a DNA copy of the viral RNA in a plasmid and then when you put that into cells it gives rise to the virus as well.

01:59:31.440 --> 01:59:37.440
You can either put the DNA into cells or you can make RNA in vitro and put that into cells and it's infectious.

01:59:37.440 --> 01:59:40.440
So again this is the modern way we do genetics in virology.

01:59:40.440 --> 01:59:45.440
The modern way we do genetics. This is the modern way we do virology.

01:59:45.440 --> 01:59:48.440
Because before that there was barely any virology.

01:59:48.440 --> 01:59:53.440
Before this there were barely any viruses you could work on.

01:59:55.440 --> 02:00:00.440
And once you see this truth you will realize that they realize that a pandemic wasn't possible.

02:00:00.440 --> 02:00:08.440
Pandemics don't happen and so they needed to create the mythological biology, the immunomythology that would underlie a pandemic.

02:00:08.440 --> 02:00:12.440
And they needed to create that illusion over many years.

02:00:12.440 --> 02:00:20.440
With many established voices that could establish an illusion of consensus about how much of this biology was not our own.

02:00:20.440 --> 02:00:32.440
Did not reflect anything about our own irreducible complexity but rather about the dangerous irreducible complexities that are outside of us that we have to survive with the help of public health.

02:00:33.440 --> 02:00:36.440
We hear about this all the time.

02:00:36.440 --> 02:00:41.440
We're going to talk about experiments where we make X modification to viral genomes.

02:00:41.440 --> 02:00:45.440
It's all going to be done with infectious DNA clones.

02:00:45.440 --> 02:00:48.440
This is the way you do it for influenza virus.

02:00:48.440 --> 02:00:52.440
I show you this because I want to tell you what was done with this technique.

02:00:52.440 --> 02:00:56.440
Influenza virus has eight RNA segments.

02:00:56.440 --> 02:01:01.440
So you have to make a plasmid for each of the eight RNA segments.

02:01:01.440 --> 02:01:06.440
And these plasmids are very trickily made. They actually have two promoters in them.

02:01:06.440 --> 02:01:12.440
All two promoter going in one direction and a Paul one promoter going in the other.

02:01:12.440 --> 02:01:16.440
He is Earth's virologist. Of course he's the gatekeeper for this.

02:01:16.440 --> 02:01:23.440
That's why he started making videos before it was cool to make videos.

02:01:23.440 --> 02:01:25.440
Because he's an op.

02:01:25.440 --> 02:01:29.440
It's not because he loves viruses so much he decided to make a podcast.

02:01:29.440 --> 02:01:33.440
And then he decided to put all his lectures online.

02:01:35.440 --> 02:01:39.440
It's because he's intended to be Earth's virology professor.

02:01:39.440 --> 02:01:43.440
That's why he goes everywhere. That's why he has a nonprofit.

02:01:43.440 --> 02:01:47.440
That's why he, I mean this is extraordinary but it's true.

02:01:47.440 --> 02:01:51.440
There are players and you can see them now and this is a bigger one.

02:01:51.440 --> 02:01:56.440
Because he's connected to one of the deepest of the deep dark people in this.

02:01:56.440 --> 02:01:59.440
David Baltimore.

02:01:59.440 --> 02:02:06.440
And when you are connected to one of these people like Bob Gallo or Murray Gardner or David Baltimore

02:02:06.440 --> 02:02:14.440
or Hilary Kropowski or Stanley Plotkin, you are connected to some of the darkest narratives in American history

02:02:15.440 --> 02:02:22.440
that are at the root cause of the public health disaster that we experienced at the root cause

02:02:22.440 --> 02:02:25.440
of the criminal vaccine schedule.

02:02:25.440 --> 02:02:35.440
Those people, if you are connected to them in your intellectual inheritance,

02:02:35.440 --> 02:02:39.440
you are part of the problem. You are part of the machine.

02:02:39.440 --> 02:02:45.440
You are part of this dark thing that we need to take away, pull our children away from.

02:02:48.440 --> 02:02:50.440
I got about 10 more minutes. I hope I can get through this.

02:02:50.440 --> 02:02:53.440
This plasmid into a cell.

02:02:53.440 --> 02:02:55.440
It goes in the nucleus.

02:02:55.440 --> 02:03:00.440
Paul one initiates here and makes the viral RNAs which are negative strand.

02:03:00.440 --> 02:03:04.440
Paul two initiates here and makes mRNA's which make proteins.

02:03:05.440 --> 02:03:10.440
So you take eight plasmids, you put them in a cell and you get influenza virus out.

02:03:10.440 --> 02:03:13.440
So you can recreate any influenza virus that you want.

02:03:13.440 --> 02:03:19.440
So it's not as simple as Fropolio because you need eight plasmids but it still can be done.

02:03:19.440 --> 02:03:24.440
Now back in 1918, as many of you know, there was a big pandemic.

02:03:24.440 --> 02:03:30.440
No explanation about how those eight segregate into eight, the eight right ones get in the thing.

02:03:30.440 --> 02:03:34.440
You know, none of that. We don't need to explain how they also get an enzyme or two in there.

02:03:34.440 --> 02:03:39.440
None of that needs to be explained. It's just obvious because they showed you the experiments.

02:03:39.440 --> 02:03:45.440
The results are there and as long as it's his results and you know, it's oh my gosh, it's awful.

02:03:45.440 --> 02:03:49.440
Well, Spanish influenza. Many millions of people died.

02:03:49.440 --> 02:03:52.440
But we didn't have influenza virus at the time.

02:03:52.440 --> 02:03:58.440
We didn't actually know it was a virus. Human influenza wasn't isolated until 1933.

02:03:58.440 --> 02:04:02.440
So we didn't have this virus which was so devastating, we couldn't study it.

02:04:02.440 --> 02:04:07.440
When people developed this eight plasmid transfection technique.

02:04:07.440 --> 02:04:11.440
How would we know that it was not a bacteria?

02:04:11.440 --> 02:04:21.440
How would we know that this is not the spread of a particularly pathogenic bacterial infection?

02:04:21.440 --> 02:04:26.440
How would we know the difference if we never even isolated it until 20 years later?

02:04:26.440 --> 02:04:35.440
What they then did was say, let's get the sequence of the 1918 virus and put it into plasmids and make that virus back again.

02:04:35.440 --> 02:04:37.440
So how did they do this?

02:04:37.440 --> 02:04:42.440
So it turned out that a lot of the people who died of influenza in 1918 got frozen.

02:04:42.440 --> 02:04:47.440
People in the Army, right? So the Army kept a lot of their pathology specimens from their lungs.

02:04:47.440 --> 02:04:52.440
They would take their lungs out and make blocks and fix them and embed them in paraffin.

02:04:52.440 --> 02:04:53.440
Great.

02:04:53.440 --> 02:04:57.440
So they had all those stored somewhere. So the investigators went down to them.

02:04:57.440 --> 02:04:58.440
Stored somewhere.

02:04:58.440 --> 02:05:05.440
You know like the room where they put Indiana Jones is where they put the law of lost art.

02:05:05.440 --> 02:05:08.440
They also had all these pathology specimens stored somewhere.

02:05:08.440 --> 02:05:12.440
They were able to sequence the viral RNA from it.

02:05:12.440 --> 02:05:15.440
They got most of the RNA genome sequence from this 1918 virus.

02:05:15.440 --> 02:05:18.440
So these are soldiers who had died from 1918 flu.

02:05:18.440 --> 02:05:25.440
But they didn't get the whole thing. So what they also did, they went up to the permafrost up in Alaska where people had died.

02:05:25.440 --> 02:05:27.440
It was an outbreak of 1918.

02:05:27.440 --> 02:05:36.440
I'm just curious. Are they suggesting that the RNA from a flu virus was able to last for that long? The RNA? That's impressive.

02:05:36.440 --> 02:05:39.440
I didn't think RNA could last that long.

02:05:39.440 --> 02:05:44.440
People had died and they were buried and had been frozen in the ground since 1918.

02:05:44.440 --> 02:05:47.440
And they opened up the graves. They did a biopsy of the lung.

02:05:47.440 --> 02:05:50.440
And they pulled out tissue. This is all done with approval, of course.

02:05:50.440 --> 02:05:53.440
They didn't do it surreptitiously at night.

02:05:53.440 --> 02:05:56.440
And then they got RNA out of these biopsies and sequenced it.

02:05:56.440 --> 02:06:01.440
And they completed the whole sequence. So then they put the sequences into each of the eight plasmids.

02:06:01.440 --> 02:06:06.440
They put the plasmids in cells. And now we have the 1918 influenza virus.

02:06:06.440 --> 02:06:11.440
This has to be worked at under BSL4 conditions because it's highly lethal.

02:06:11.440 --> 02:06:16.440
Every animal that you put this in, mice, ferrets, guinea pigs, they're wiped out.

02:06:16.440 --> 02:06:20.440
So we can understand what made this virus lethal.

02:06:20.440 --> 02:06:23.440
It wipes them all out.

02:06:23.440 --> 02:06:28.440
Somehow I didn't think influenza had such a universal, you know, you can put it in everything.

02:06:28.440 --> 02:06:33.440
Or do they make a shit ton of it using infectious clones and then you can put it in everything.

02:06:33.440 --> 02:06:36.440
Do you see the difference there? It's so funny.

02:06:36.440 --> 02:06:40.440
I mean, once you see it for what it is, it's just a patent set of lies.

02:06:40.440 --> 02:06:45.440
I mean, you can't bring it in this way. Many people think we should not have done this experiment

02:06:45.440 --> 02:06:50.440
because these sequences are published. So anyone could go into GenBank and get these sequences

02:06:50.440 --> 02:06:54.440
and make this virus if you wanted to do that and make a weapon, for example.

02:06:54.440 --> 02:06:59.440
Kim.com is terrified of that. The next virus is going to kill a billion people.

02:06:59.440 --> 02:07:03.440
It's much better than nuclear weapons because you can do it in your garage.

02:07:03.440 --> 02:07:09.440
Robert Malone was actually in front of the UK parliament, or at least in a room in the UK parliament,

02:07:09.440 --> 02:07:14.440
and told all the people in the room that it is possible for someone with a bachelor's degree in biology

02:07:14.440 --> 02:07:18.440
to go on eBay and get the equipment necessary to assemble,

02:07:18.440 --> 02:07:21.440
hey, gain a function coronavirus in their freaking garage.

02:07:21.440 --> 02:07:24.440
So lucky I decided to start streaming, right?

02:07:24.440 --> 02:07:27.440
And started to use my handwriting to teach biology.

02:07:27.440 --> 02:07:32.440
Otherwise, I could get up to a lot of nastier things in here. Holy cow.

02:07:32.440 --> 02:07:38.440
But I think that risk is pretty low. I think the benefits outweigh this, the kind of research that we can do.

02:07:38.440 --> 02:07:44.440
All right. The last thing I want to tell you about is good uses of viruses for curing human diseases.

02:07:44.440 --> 02:07:48.440
We have been able to make viral vectors and put foreign genes into them.

02:07:48.440 --> 02:07:55.440
And so these are used for gene therapy. There are lots of diseases where patients lack a specific gene.

02:07:55.440 --> 02:08:00.440
And so one of the most common approaches is to take cells from these patients.

02:08:00.440 --> 02:08:03.440
Now we're beginning to take bone marrow stem cells out.

02:08:03.440 --> 02:08:07.440
You infect them with a virus that will supply the gene that's missing,

02:08:07.440 --> 02:08:11.440
and then you infuse these cells back into the patient.

02:08:11.440 --> 02:08:17.440
And that doesn't work very well for very many things, including sickle cell anemia,

02:08:17.440 --> 02:08:20.440
which they are not, we're doing it, but people are not.

02:08:20.440 --> 02:08:25.440
It's not working out. Didn't work out for Jesse Gelsinger.

02:08:25.440 --> 02:08:29.440
And Robert Malone got three New York Times articles out of that,

02:08:29.440 --> 02:08:33.440
and then a breast cancer institute or something like that,

02:08:33.440 --> 02:08:35.440
even though he calls that burning his career.

02:08:35.440 --> 02:08:38.440
Of course, you need to use a virus that will not kill these cells,

02:08:38.440 --> 02:08:42.440
and there are plenty of non-lidic viruses that we can work with.

02:08:42.440 --> 02:08:47.440
Non-lidic, non-lidic viruses are viruses that don't explode the cell

02:08:47.440 --> 02:08:51.440
after they make so many copies of themselves. Just in case you missed that term,

02:08:51.440 --> 02:08:55.440
that's a term that comes back from David Baltimore's lecture a couple of days ago

02:08:55.440 --> 02:08:59.440
where he said that one of the ways that viruses can leave a cell

02:08:59.440 --> 02:09:03.440
is to bud off the outside, and in that case I guess all of the receptors

02:09:03.440 --> 02:09:08.440
that the virus has have to first be on the outside of the host cell,

02:09:08.440 --> 02:09:13.440
and then virus genomes have to go up to the cell surface

02:09:13.440 --> 02:09:17.440
and bud off and be covered with receptors and then packaged correctly.

02:09:17.440 --> 02:09:20.440
And then there are other models that are more complicated than that

02:09:20.440 --> 02:09:24.440
and that include just making so much viruses that the cell explodes.

02:09:24.440 --> 02:09:29.440
And those are lidic viruses, and so non-lidic viruses are viruses

02:09:29.440 --> 02:09:34.440
that are more subtle or have more class and don't ruin the host cell,

02:09:34.440 --> 02:09:37.440
but just bud off of them.

02:09:37.440 --> 02:09:41.440
But a lot of these have been developed and are being used in very small trials

02:09:41.440 --> 02:09:45.440
to treat a variety of diseases. We also use them in research as well.

02:09:45.440 --> 02:09:49.440
There are some genetic diseases that are amenable to gene therapy.

02:09:49.440 --> 02:09:55.440
For example, skids, severe combined immunodeficiency is a defect in a very specific gene.

02:09:55.440 --> 02:09:58.440
So when you have a one gene defect, you can think about repairing it.

02:09:58.440 --> 02:10:01.440
Factor eight, factor seven, and factor nine deficiency.

02:10:01.440 --> 02:10:05.440
When you have a one gene deficiency, yes, you can think about repairing it.

02:10:05.440 --> 02:10:08.440
You definitely can imagine lots of ways to think about it.

02:10:08.440 --> 02:10:12.440
LDL resetocystic fibrosis is a defect in a channel.

02:10:12.440 --> 02:10:14.440
Smokes matter.

02:10:14.440 --> 02:10:21.440
So a lot of these and other gene defects are being corrected or trying to correct with gene therapy.

02:10:21.440 --> 02:10:26.440
One of the most common vectors that's used is retroviral vectors.

02:10:26.440 --> 02:10:30.440
The other one are the single-stranded DNA viruses like parvoviruses.

02:10:30.440 --> 02:10:32.440
Those are being used as well.

02:10:32.440 --> 02:10:36.440
Now a lot of this won't make sense to you yet because we haven't covered retroviruses,

02:10:36.440 --> 02:10:41.440
but what you do if you want to make a retrovirus to do gene therapy

02:10:41.440 --> 02:10:44.440
is you put the genome into two plasmids.

02:10:44.440 --> 02:10:51.440
You have a plasmid that has the coding region to make the capsid proteins

02:10:51.440 --> 02:10:55.440
and you have another plasmid with what's called the envelope proteins.

02:10:55.440 --> 02:10:58.440
These are the glycoproteins that will be in the membrane of the virus.

02:10:58.440 --> 02:11:02.440
Now if you take just these two plasmids and you transfect them into cells,

02:11:02.440 --> 02:11:05.440
you get empty retrovirus particles.

02:11:05.440 --> 02:11:08.440
There's no genome in there because you haven't inserted one.

02:11:08.440 --> 02:11:10.440
You've just made a particle.

02:11:10.440 --> 02:11:12.440
Now let's say you want to insert a gene in here.

02:11:12.440 --> 02:11:16.440
You just take the same two plasmids and then you add a third plasmid

02:11:16.440 --> 02:11:18.440
containing your gene of interest.

02:11:18.440 --> 02:11:21.440
You want to correct the gene defect, you put the gene in here,

02:11:21.440 --> 02:11:25.440
and you put signals in it so it will be incorporated into the retrovirus.

02:11:25.440 --> 02:11:27.440
So three plasmids you transfect.

02:11:27.440 --> 02:11:28.440
And they know those signals.

02:11:28.440 --> 02:11:30.440
See, they know those signals.

02:11:30.440 --> 02:11:31.440
They figured out those signals.

02:11:31.440 --> 02:11:34.440
So they know that if they combine these signals,

02:11:34.440 --> 02:11:39.440
so they figured out how exosomes are made.

02:11:40.440 --> 02:11:44.440
You see, they figured this out and they call it pathology.

02:11:44.440 --> 02:11:49.440
They call it viruses so that it's exclusively their intellectual property,

02:11:49.440 --> 02:11:51.440
their intellectual space.

02:11:51.440 --> 02:11:56.440
And then this mythology will be used to enslave our children by telling them a story

02:11:56.440 --> 02:12:00.440
that's actually about their own sacred, irreducible complexity,

02:12:00.440 --> 02:12:06.440
the biology that defines them and defines all of us as what we are

02:12:06.440 --> 02:12:11.440
and calling it pathology and calling it obligate pathogens from alien places

02:12:11.440 --> 02:12:15.440
or a pre-primordial world.

02:12:15.440 --> 02:12:16.440
Inspect them in cells.

02:12:16.440 --> 02:12:19.440
You now have a retrovirus containing a foreign gene.

02:12:19.440 --> 02:12:23.440
And you can use that to try and treat genetic diseases.

02:12:23.440 --> 02:12:27.440
Now these vectors, of course, are gutted so they don't cause any disease.

02:12:27.440 --> 02:12:30.440
They do integrate into your DNA.

02:12:30.440 --> 02:12:34.440
And there have been some cases where individuals have had tumors arise

02:12:34.440 --> 02:12:38.440
because these vectors, they integrate rather randomly,

02:12:38.440 --> 02:12:41.440
they integrate next to an oncogene and activate it.

02:12:41.440 --> 02:12:42.440
And so tumors arise.

02:12:42.440 --> 02:12:43.440
So there's a lot of work around.

02:12:43.440 --> 02:12:48.440
I mean, there's even oncogene is already probably a biological term

02:12:48.440 --> 02:12:50.440
that's going to go to the wayside at some point.

02:12:50.440 --> 02:12:54.440
Although I haven't bothered to play with that one.

02:12:54.440 --> 02:12:58.440
That we need to do still to make these work better.

02:12:58.440 --> 02:13:02.440
But retroviruses, parval viruses, and a couple of other viruses

02:13:02.440 --> 02:13:06.440
being increasingly used and maybe in 10 to 15 years,

02:13:06.440 --> 02:13:07.440
this will be commonplace.

02:13:07.440 --> 02:13:08.440
Identiviruses.

02:13:08.440 --> 02:13:11.440
Maybe, maybe, you know, like Robert Malone said,

02:13:11.440 --> 02:13:15.440
when he was in grad school and doing his stuff with Marie Gardner

02:13:15.440 --> 02:13:19.440
and with ender verma, he was pretty convinced that within a decade

02:13:19.440 --> 02:13:23.440
there would be a geneticist at every hospital carrying childhood diseases

02:13:23.440 --> 02:13:24.440
with retroviruses.

02:13:24.440 --> 02:13:27.440
Unfortunately, he was wrong about that.

02:13:27.440 --> 02:13:31.440
Unfortunately, that seems to be an intricate part of this mythology.

02:13:31.440 --> 02:13:35.440
A promise that can never be reached, but seems so right there.

02:13:35.440 --> 02:13:38.440
Just over the horizon, just in 10 years, we're going to get there.

02:13:38.440 --> 02:13:41.440
And people like Kurt Zweiler think that if he can just leave,

02:13:41.440 --> 02:13:45.440
make it until 2040, he can have his consciousness uploaded

02:13:45.440 --> 02:13:49.440
or replicated or cloned or whatever, and he's going to live forever.

02:13:53.440 --> 02:13:54.440
Here's an example.

02:13:54.440 --> 02:13:57.440
Exlinked adrenal leukodystrophy.

02:13:57.440 --> 02:14:01.440
So this is a CNS disease, typically fatal.

02:14:01.440 --> 02:14:03.440
It's a defect in a transporter called ABCD1.

02:14:03.440 --> 02:14:06.440
And in this trial, a couple of two patients, two children with this disease.

02:14:06.440 --> 02:14:07.440
Their bone marrow is taken out.

02:14:07.440 --> 02:14:09.440
It was infected in culture with a antiviral or retroviral vector

02:14:09.440 --> 02:14:12.440
with a normal gene, and then they infused these cells back into the patients,

02:14:12.440 --> 02:14:13.440
and their state is stabilized.

02:14:13.440 --> 02:14:15.440
They stopped degenerating, and one of them, I think, improved.

02:14:15.440 --> 02:14:17.440
So a small clinical trial, but just an example of what you can do with the virus.

02:14:17.440 --> 02:14:19.440
I bet they're dead now.

02:14:21.440 --> 02:14:23.440
Hey, nice, nice way to end it.

02:14:23.440 --> 02:14:25.440
I guess they're dead now.

02:14:26.440 --> 02:14:30.440
Anyway, ladies and gentlemen, this has been giga-owned biological,

02:14:30.440 --> 02:14:34.440
a high-resistance, low-noise information brief brought to you by a biologist.

02:14:36.440 --> 02:14:41.440
We were doing Vincent Rancin yellow and virology 103,

02:14:41.440 --> 02:14:45.440
and this is probably the last day that I'm going to do a study hall like this on virology.

02:14:45.440 --> 02:14:49.440
I think now it's time for us to make a little bit of a summary of this stuff

02:14:49.440 --> 02:14:52.440
and make a little bit of a summary on prions and that kind of thing.

02:14:52.440 --> 02:14:56.440
I might come back later today and cover one of these prion videos

02:14:56.440 --> 02:15:00.440
from either Stephanie Sineff or Kevin McCarron,

02:15:00.440 --> 02:15:06.440
only just to see what we can add to our understanding of their implication

02:15:06.440 --> 02:15:07.440
of what's going on here.

02:15:07.440 --> 02:15:11.440
I suspect that it's really a spike protein-centered mythology,

02:15:11.440 --> 02:15:14.440
and it's not really a genuine presentation,

02:15:14.440 --> 02:15:17.440
but I'm willing to listen, and I'm willing to see what they're talking about now,

02:15:17.440 --> 02:15:21.440
because I haven't been paying attention to them for a very long time,

02:15:21.440 --> 02:15:27.440
and I did notice that Stephanie Sineff a couple days ago was actually on Kevin McCarron's broadcast,

02:15:27.440 --> 02:15:33.440
which I think is an extraordinary milestone for us to reach in this narrative.

02:15:33.440 --> 02:15:38.440
Ladies and gentlemen, I think that this hypothesis is still extraordinarily valid.

02:15:38.440 --> 02:15:42.440
I think that they told us that there was a dangerous novel virus, but there wasn't.

02:15:42.440 --> 02:15:43.440
It may have been a background.

02:15:43.440 --> 02:15:46.440
It may have been a combination of a background and bad testing.

02:15:46.440 --> 02:15:48.440
It is not about profit, though.

02:15:48.440 --> 02:15:52.440
It is about control, and specifically, it is about controlling our grandchildren

02:15:52.440 --> 02:15:58.440
with a mythology about public health, a mythology about obligate pathogens in the wild

02:15:58.440 --> 02:16:02.440
that have nothing to do with our own irreducible complexity

02:16:02.440 --> 02:16:06.440
that includes exosomal signaling in health and disease.

02:16:06.440 --> 02:16:11.440
Financial incentives and other perks were put in place

02:16:11.440 --> 02:16:14.440
so that people would be coerced into accepting this,

02:16:14.440 --> 02:16:19.440
and very specifically, a national security event occurred.

02:16:19.440 --> 02:16:23.440
And in the context of that national security event, people were co-opted.

02:16:23.440 --> 02:16:25.440
People were told they had to participate.

02:16:25.440 --> 02:16:28.440
People were told that certain parts of the narrative could not be touched,

02:16:28.440 --> 02:16:33.440
because the security of the world, the economic system was in danger

02:16:33.440 --> 02:16:36.440
and that we needed maximum compliance.

02:16:36.440 --> 02:16:41.440
And in participating in this narrative, they helped coerce hundreds of millions of people

02:16:41.440 --> 02:16:45.440
around the world into accepting multiple transfection events

02:16:45.440 --> 02:16:47.440
that probably have permanently damaged their bodies.

02:16:47.440 --> 02:16:54.440
And now, they would like to tell you a story about a virology, a viral protein,

02:16:54.440 --> 02:17:01.440
a mythology that will essentially absolve this plan and its executors

02:17:01.440 --> 02:17:05.440
from the guilty verdict that should befall them,

02:17:05.440 --> 02:17:10.440
that they were guilty of spreading a mythology that they knew was going to kill people,

02:17:10.440 --> 02:17:17.440
that they covered up this in order to execute this national security exercise,

02:17:17.440 --> 02:17:24.440
where maximum compliance meant that the maximum number of people accepted a novel virus

02:17:24.440 --> 02:17:28.440
that killed millions of people that transfection was necessary for

02:17:28.440 --> 02:17:32.440
and quite effective in treating if it wasn't for rushing it

02:17:32.440 --> 02:17:36.440
and that because it was gained a function, it could come again.

02:17:36.440 --> 02:17:39.440
Ladies and gentlemen, this has been Gigome Biological, a high-resistance,

02:17:39.440 --> 02:17:42.440
low-noise information brief brought to you by biologists.

02:17:42.440 --> 02:17:46.440
If you like what you hear, you can become a subscriber at GigomeBiological.com

02:17:46.440 --> 02:17:53.440
for as little as $10 a month, or you can set up a recurring payment on PayPal for $1 a month.

02:17:53.440 --> 02:17:59.440
We're going to be here every day, and my family's going to find a way, one way or another,

02:17:59.440 --> 02:18:05.440
and that's what we do here, so I'll see you again tomorrow at 10.10.

02:18:05.440 --> 02:18:11.440
And if you want to, you can try to find Fearla on YouTube today at 1 o'clock

02:18:11.440 --> 02:18:15.440
because she will be doing a live yoga class, but I'm not going to promote that here.

02:18:15.440 --> 02:18:20.440
That's something completely separate, but if you're interested in that, it's not too hard to find.

02:18:20.440 --> 02:18:26.440
Thank you very much for joining me, and I'll see you again tomorrow at 10.10. Try to be on time. I will be too.

02:18:31.440 --> 02:18:33.440
I did that wrong. Darn it.

02:18:35.440 --> 02:18:37.440
I did that wrong.

02:19:05.440 --> 02:19:07.440
I did that wrong.

02:19:35.440 --> 02:19:37.440
You

